Sodium Pyruvate Therapy in COPD Patients

Long-Term Use of Inhaled Sodium Pyruvate for the Treatment of Chronic Obstructive Pulmonary Disease

In animal models, sodium pyruvate has been shown to be an effective anti-inflammatory agent, and in human studies sodium pyruvate has been shown to be a bronchodilating agent. Subjects with COPD are known to have inflammation in the lung, and often have bronchoconstriction. As such, these subjects typically are on multiple therapies, including steroid therapy. This trial will study the effect of inhaled sodium pyruvate on inflammation and lung function in COPD subjects over a six week period.

Study Overview

• Status: Completed
• Conditions: Chronic Obstructive Pulmonary Disease (COPD)
• Intervention / Treatment: Drug: sodium pyruvate in 0.9% sodium chloride solution

Detailed Description

Chronic obstructive pulmonary disease (COPD) is a disease, which involves the conducting airways of the respiratory tract and affects 5-10% of individuals in the United States. The morbidity and mortality associated with this disease are increasing both in the United States and worldwide (1). The mechanisms involved in the pathogenesis of COPD are complex but it can be classified as an inflammatory disease of the airways characterized by an increase in inflammatory cells (neutrophils, macrophages, and lymphocytes) both in the bronchial mucosa and in bronchoalvaeolar lavage fluid (2). Some of the many therapeutic approaches to managing this disease include various inhaled compounds, such as, bronchodilators (i.e. beta agonists) and both inhaled and systemic steroid treatment. The current therapies are not without adverse side effects. The purpose of this study is to test the safety and therapeutic value of an endogenous compound, sodium pyruvate, with anti-inflammatory properties (anti-oxidant) in patients with COPD.

Reactive oxygen species (ROS), such as superoxide anion, free hydroxyl radical, and hydrogen peroxide, have been shown to be toxic to various mammalian tissue (3), including lung (4--6), and have been implicated in many human diseases (7). Anti- oxidant therapy has been shown to be effective in several animal models of inflammatory lung diseases (8-10). Sodium pyruvate is a part of the body's natural endogenous anti-oxidant defense system. It is secreted by cells, readily enters cells, and can react with peroxide to "detoxify it (11-13). Since hydrogen peroxide is also a precursor to other ROS, inhibition of it has broad anti-oxidant effects. Sodium pyruvate has been shown to have protective anti-oxidant activity both in vitro (11, 14-17) and in vivo (18, 19).

Clinically, sodium pyruvate has been given to patients for a variety of disorders ranging from Friedreich's ataxia (20) to open heart operations (21). It has been administered via several routes including intravenous (20-23), topical administration for hyperkeratotic disorders (24), and in dietary supplementation (25-29). Animal model studies in rats and rabbits were conducted to assess potential adverse effects. Rats were used to assess the impact of sodium pyruvate administered directly to the lungs by intratracheal injection (Rat Study). Rabbits were used to assess the impact of sodium pyruvate administered as an inhaled mist using a nebulizer (Pyruvate Inhalation Study in Rabbits). There were no adverse effects noted in the parameters, which were monitored for acute lung injury (histology, bronchoalveolar lavage fluid content, and arterial blood gas analysis). In addition, it was shown that the intratrachcheal administration of sodium pyruvate was beneficial in diminishing the development of acute lung injury in rats induced by bleomycin. The generation of toxic oxygen radicals is believed to be a major mechanism of bleomycin lung toxicity.

There are two major groups of COPD medications: 1) Bronchodilator agents that inhibit the bronchoconstriction characteristic of some patients with COPD; and 2) anti-inflammatory agents which inhibit the inflammatory components responsible for the airway narrowing and increased mucus production, also characteristic features of patients with COPD. The known anti-oxidant properties of sodium pyruvate make it an attractive compound for possible therapeutic efficacy, which should have in vivo anti-inflammatory effects. In addition to its possible anti-inflammatory properties, we previously observed (Phase I study of this compound, see Phase I report) acute therapeutic efficacy in patients with mild bronchial asthma. The observed improvement in FEV1 at 30 minutes to 2 hours post inhalation of sodium pyruvate suggests a bronchodilator effect in these patients. The mechanism of action is unclear at this time but it may be a result of the ability of inhaled sodium pyruvate to modulate reactive oxygen and nitrogen species such as H2O2 and nitric oxide (NO). These species have both been shown to be elevated in expired breath of patients with COPD and asthma and thus implicated in the pathogenesis (30-39). Nitric oxide has been shown to play an important role in maintaining normal physiologic homeostasis in the normal and injured lung (40-42). It has been shown to reverse acetylcholine and histamine induced bronchoconstriction and to maintain normal vascular integrity (40-42). It is possible that inhaled sodium pyruvate alters airway levels of both oxygen and nitrogen reactive species resulting in a beneficial effect.

Since sodium pyruvate is endogenous, has been used previously in patients, had no adverse affects in our animal model studies, and had no adverse effects in our acute Phase I Clinical Trial in patients with mild bronchial asthma, we feel that we will be able to use it safely in this long term multiple use study in patients with COPD. For safety considerations only the lowest concentration of sodium pyruvate (0.5mM) studied previously in our single dose Safety Trial will be studied in this 3-times/day double blind placebo controlled 6 week study. The total daily dose per subject will be similar to the 1.5mM concentration (3 doses of 0.5mM). In addition, it is approximately 3 times less than the total daily dose given to subjects who received the 5.0mM concentration in our Single Dose Phase I Safety Trial. The proposed study is designed to address both the long term multiple dose safety aspects of inhaled sodium pyruvate as well as both the acute and long-term efficacy of the compound. The acute study is designed to evaluate the possible bronchodilator effects and the long term multiple use study is designed to evaluate the anti-inflammatory properties of sodium pyruvate.

• Study Type: Interventional
• Enrollment: 60
• Phase: Phase 2

Contacts and Locations

Study Locations

• United States
  • Connecticut
    • New Britain, Connecticut, United States, 06053
      • Hospital for Special Care
    • New Haven, Connecticut, United States, 06520-8057
      • Yale University School of Medicine

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 85 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Individuals with a clinical diagnosis of moderate to severe COPD, <70% predicted FEV1, and a stable pulmonary disease status.

Exclusion Criteria:

• a. Pulmonary disease other than COPD
• b. Clinically significant cardiac disease including uncontrolled congestive heart failure and unstable angina
• c. Pregnancy
• d. Females of child bearing potential age not on adequate contraception or lactating
• e. Subjects receiving oral or IV corticosteroid treatment within one month of Screening Visit
• f. Less than 18 years of age and greater than 85 years of age
• g. Hospitalization within last 6 months due to acute exacerbation of air-way disease
• h. Subjects on escalating dose of immunotherapy
• i. Subjects with a clinically significant abnormal chest x-ray within past 12 months.
• j. Medication changes within 1 month
• k. Subjects who have participated in another investigation drug treatment study within the previous month.
• l. Subjects with a current history of alcohol abuse or recreational drug use.
• m. Subjects who have taken vitamins with anti-oxidant properties (E or C) or dietary supplements containing pyruvate within 24 hours prior to the screening visit.
• n. Subjects who use less than 4 puffs of Albuterol a day over the period between the Screening Visit and Visit 1.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Lung function, as determined by spirometry
Safety, as determined by laboratory analyses

Secondary Outcome Measures

Outcome Measure
Levels of Nitric Oxide in expired air
Results of Chronic Respiratory Disease Questionnaire
SaO2 levels

Collaborators and Investigators

• Sponsor: Emphycorp
• Collaborators: Cellular Sciences, inc.
• Investigators: Principal Investigator: John J. Votto, D.O., Hospital for Special Care

Publications and helpful links

Helpful Links

• Emphycorp Website

Study record dates

Study Major Dates

• Study Start: September 1, 2004
• Study Completion: May 1, 2006

Study Registration Dates

• First Submitted: December 6, 2005
• First Submitted That Met QC Criteria: December 6, 2005
• First Posted (Estimate): December 7, 2005

Study Record Updates

• Last Update Posted (Estimate): May 29, 2006
• Last Update Submitted That Met QC Criteria: May 25, 2006
• Last Verified: May 1, 2006

More Information

Terms related to this study

• Keywords: COPD; Chronic Obstructive Pulmonary Disease; Sodium Pyruvate
• Additional Relevant MeSH Terms: Respiratory Tract Diseases; Lung Diseases; Lung Diseases, Obstructive; Pulmonary Disease, Chronic Obstructive
• Other Study ID Numbers: CSI-NII5-021