The Evaluation of Thiol Redox Status in Conjunctiva of Dry Eye Patients

Dry eye disease is related with oxidative stress at the ocular surface. GSH is one of the key factor of protect from oxidative stress. To evaluate thiol status of ocular surface cells by monitoring GSH concentration, we can evaluate the severity of dry eye.

Study Overview

• Status: Unknown
• Conditions: Dry Eye Disease

Detailed Description

The conjunctival cells are collected by impression cytology. Those cells are stained with 100 mM of monochlorobimane (MCB, M-1381, Molecular Probes, Eugene, OR) and propidium iodide (PI). Fluorescent intensity, reflecting the amount of icGSH, was inspected under a confocal laser scan microscope. To detect the GSH levels we used a fluorescent MCB probe with excitation and emission wavelengths of 405 nm and 410-480 nm, respectively. The cell-permeating MCB probe is non-fluorescent but forms a fluorescent adduct with GSH in a reaction catalyzed by glutathione-S-transferase.

• Study Type: Observational
• Enrollment (Anticipated): 50

Contacts and Locations

• Study Contact: Name: Jun Yamada, MD, PhD.; Phone Number: +81-75-251-5663; Email: jyamada@koto.kpu-m.ac.jp
• Study Contact Backup: Name: Norihiko Yokoi, MD, PhD.; Phone Number: +81-75-251-5663; Email: nyokoi@koto.kpu-m.ac.jp

Study Locations

• Japan
  • Kyoto, Japan
    • Kyoto Prefectural University of Medicine hospital
    • Contact: Shigeru Kinoshita, MD, PhD.; Phone Number: +81-75-251-5577; Email: shigeruk@koto.kpu-m.ac.jp

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

The dry eye patients at the clinic of Kyoto Prefectural University of Medicine hospital

Description

Inclusion Criteria:

• dry eye patients
• control volunteers

Exclusion Criteria:

• Young (less than 20) patients
• The patients who rejected this study

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control

Number of groups / cohorts

4

Cohorts and Interventions

Group / Cohort
Sjogren; Sjogren syndrome with dry eye
non-Sjogren; dry eye without Sjogren syndrome
Short-BUT; Dry eye by shortened tear break up time
control; normal patients

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Tear amount, tear break up time, and so on are measured clinically. The thiol redox status is measured by staining the membrane.	Once a month (totally 2-4 times) at the clinic

Collaborators and Investigators

• Sponsor: Meiji University of Oriental Medicine
• Collaborators: Kyoto Prefectural University of Medicine

Study record dates

Study Major Dates

• Study Start: August 1, 2008
• Primary Completion (Anticipated): December 1, 2008
• Study Completion (Anticipated): March 1, 2010

Study Registration Dates

• First Submitted: July 18, 2008
• First Submitted That Met QC Criteria: July 18, 2008
• First Posted (Estimate): July 21, 2008

Study Record Updates

• Last Update Posted (Estimate): July 21, 2008
• Last Update Submitted That Met QC Criteria: July 18, 2008
• Last Verified: May 1, 2008

More Information

Terms related to this study

• Keywords: Oxidative stress; dry eye; Thiol redox
• Additional Relevant MeSH Terms: Lacrimal Apparatus Diseases; Keratoconjunctivitis; Conjunctivitis; Conjunctival Diseases; Keratitis; Corneal Diseases; Eye Diseases; Dry Eye Syndromes; Keratoconjunctivitis Sicca
• Other Study ID Numbers: C244