Effect of Amino Acid on Muscle Damage

Effect of Leucine-enriched Essential Amino Acid on Integrated Muscle Protein Synthesis and Muscle Recovery After Resistance Exercise

The primary objective is to determine the impact of essential amino acids on integrated muscle protein synthesis over 4 days after resistance exercise.

In addition, the secondary objective is to determine the effect of essential amino acids on satellite cell regulation and inflammatory responses during this prolonged recovery period.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Dietary supplement: Placebo; Dietary supplement: Active

• Study Type: Interventional
• Enrollment (Actual): 20
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Canada
  • Ontario
    • Toronto, Ontario, Canada, M5S2C9
      • Goldring Centre for High Performance Sport

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy, male, recreationally-active participants
• Healthy will be defined as screened by the PAR-Q+ (The Physical Activity Readiness Questionnaire for everyone; Appendix. B). Active will be defined as being in the top 20% of age specific scores on iPAQ (the International Physical Activity Questionnaire; Appendix.C).
• Participants are required not to engage in lower limb RT (resistance training) for at least 6 months prior to the study.
• Participants will be 18-35 years old.
• Participants are willing to abide by the compliance rules of this study

Exclusion Criteria:

• Female
• Inability to adhere to any of the compliance rules judged by principle investigator or medical doctor
• Self-reported regular tobacco use
• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.)
• The individual who has allergy for milk

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Placebo Comparator: Placebo; 4g of maltodextrin	Dietary supplement: Placebo; 4g of maltdextrin
Active Comparator: Active; 4g of essential amino acids	Dietary supplement: Active; 4g of essential amino acids

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle protein synthesis	Skeletal muscle fractional synthesis rate (%/h) will be determined by oral pulse method using ring-[D5]phenylalanine	4 hours

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
exercise performance	Maximal strength (torque in n-M) of the knee extensors will be assessed by a maximal isometric voluntary contraction (MVC).	4 days
Muscle soreness	Corresponding subjective ratings of muscle soreness will be collected from participants using a visual analogue scale (mm)	4 days
Muscle damage	Muscle damage will be determined by histochemical analysis. muscle damage is defined as Z-band streaming.	4 days

Collaborators and Investigators

• Sponsor: University of Toronto
• Collaborators: Ajinomoto Co., Inc.

Study record dates

Study Major Dates

• Study Start (Actual): October 18, 2017
• Primary Completion (Actual): January 15, 2019
• Study Completion (Actual): October 30, 2019

Study Registration Dates

• First Submitted: October 18, 2017
• First Submitted That Met QC Criteria: October 18, 2017
• First Posted (Actual): October 24, 2017

Study Record Updates

• Last Update Posted (Actual): April 20, 2021
• Last Update Submitted That Met QC Criteria: April 17, 2021
• Last Verified: April 1, 2021

More Information

Terms related to this study

• Keywords: exercise; recovery; muscle; protein
• Other Study ID Numbers: EAA

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No