Alterations in Subgingival Microbiota and Hypoxia in Occlusal Trauma

Present study suggests that excessive occlusal forces did not cause a significant change in hypoxia and vascular markers, however a slight alteration in subgingival microbiota was observed.

Study Overview

• Status: Completed
• Conditions: Chronic Periodontitis; Occlusal Trauma

Detailed Description

Occlusal trauma is defined as tissue destruction and/or injury resulting in tissue changes in the periodontium as a consequence of excessive occlusal forces or hyper-occlusion. Previous studies suggested accelerated periodontal destruction around teeth with occlusal trauma and increased mobility, however, the evidence is lacking. Recent animal studies found that occlusal trauma increased osteoclastic activity and alveolar bone loss. One possible mechanism suggested for the progression of periodontal destruction with occlusal trauma is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. However, the association of occlusal trauma and subgingival microbiota was not evaluated in previous studies. Therefore, present study aimed to evaluate subgingival dental plaque components and compare with healthy individuals and periodontitis patients without occlusal trauma.

• Study Type: Observational
• Enrollment (Actual): 80

Contacts and Locations

Study Locations

• Turkey
  • Tokat, Turkey, 60100
    • Gaziosmanpasa University Faculty of Dentistry
  • Tokat, Turkey, 60100
    • Gaziosmanpasa University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 29 years to 42 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

• Sampling Method: Probability Sample

Study Population

The study population consisted of 80 participants and 4 study groups were created as follows:

Group 1; healthy individuals without occlusal trauma (Group 1, n=20), 6 men and 14 women, mean age 32.06±2.90 Group 2; periodontitis patients without occlusal trauma (Group 2, n=20), 6 men and 14 women, mean age 36.90±4.67 Group 3; healthy individuals with occlusal trauma (Group 3, n=20), 5 men and 15 women, mean age 34.89±7.51 Group 4; periodontitis patients with occlusal trauma (Group 4, n=20) 7 men and 13 women, mean age 36.76±4.40.

Description

Inclusion Criteria:

• Systemical health
• No drug use
• No pregnancy or lactation For group 3 and 4;
• Bruxism at least for 2 years
• Occlusal trauma
• Existence of at least 20 functioning teeth

Exclusion Criteria:

• Smoking
• Pregnancy/lactation
• Systemical disease
• Drug use
• Absence of at least 20 functioning teeth

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

4

Cohorts and Interventions

Group / Cohort
healthy individuals; Orally and systemically healthy individuals who has no hypo-functional teeth, hyper-occlusion or occlusal trauma
periodontitis patients; Systemically healthy individuals who has chronic periodontitis but no hypo-functional teeth, hyper-occlusion or occlusal trauma
healthy-occlusal trauma; Orally and systemically healthy individuals who has occlusal trauma caused by bruxism
periodontitis-occlusal trauma; Systemically healthy individuals who has both chronic periodontitis and occlusal trauma caused by bruxism

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Determination of certain bacterial species in subgingival dental plaque	Subgingival dental plaque samples were collected from each participant and evaluated via polymerase-chain reaction.	A day after initial examination

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
hypoxic changes in gingiva and serum	Determination of the hypoxia inducible factor-alpha and vascular endothelial growth factor via ELISA	One day after initial examination

Collaborators and Investigators

• Sponsor: Tokat Gaziosmanpasa University
• Investigators: Principal Investigator: HATİCE BALCI YÜCE, PhD, Gaziosmanpasa University

Study record dates

Study Major Dates

• Study Start (Actual): May 1, 2016
• Primary Completion (Actual): May 1, 2017
• Study Completion (Actual): May 1, 2017

Study Registration Dates

• First Submitted: August 4, 2018
• First Submitted That Met QC Criteria: August 4, 2018
• First Posted (Actual): August 9, 2018

Study Record Updates

• Last Update Posted (Actual): August 10, 2018
• Last Update Submitted That Met QC Criteria: August 8, 2018
• Last Verified: August 1, 2018

More Information

Terms related to this study

• Keywords: periodontitis; hypoxia; hyper-occlusion; occlusal trauma; subgingival microbiota
• Additional Relevant MeSH Terms: Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Signs and Symptoms, Respiratory; Periodontitis; Wounds and Injuries; Hypoxia; Chronic Periodontitis
• Other Study ID Numbers: balciyuce84

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: Undecided

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No