Beta-Hydroxy-Beta-Methylbutyrate Supplementation and Physical Activity in Liver Cirrhosis: a Controlled Trial

Beta-Hydroxy-Beta-Methylbutyrate Supplementation and Physical Activity in Liver Cirrhosis: a Controlled Trial

Sponsors

Lead Sponsor: University of Roma La Sapienza

Source University of Roma La Sapienza
Brief Summary

Sarcopenia is an independent predictor of morbidity and mortality in the cirrhotic patient. Beta-hydroxy-beta-methyl butyrate (HMB) is a leucine metabolite with potential efficacy in increasing protein synthesis, muscle mass, and its functionality. The aim of this randomized controlled study is to evaluate the effect of nutritional supplementation with HMB and physical activity both on muscle mass and on muscle function in cirrhotic patients.

Detailed Description

1. Introduction Changes in nutritional status are a frequent complication in cirrhotic patients. The prevalence of malnutrition is related to the severity of the disease and has been reported by 65-90% in advanced cirrhosis. The most significant component of malnutrition is a progressive and generalized loss of mass understood as sarcopenia. Several studies have reported that sarcopenia represents a negative prognostic factor for survival in patients with liver cirrhosis. Other studies have shown that sarcopenia is an independent predictor of complications of portal hypertension. For these reasons and being a modifiable condition, identifying and treating sarcopenia in patients is very important for their prognosis. Sarcopenia in liver cirrhosis occurs as a result of an increase in proteolysis or a reduction in protein synthesis, or a combination of the two mechanisms. The alterations in the molecular pathways that involve the regulation of these mechanisms are not entirely known. Recent research has led to the identification of increased expression of myostatin, member of the transforming growth factor ß superfamily, in skeletal muscle in animal models of hepatic injury and in the plasma of cirrhotic patients with consequent inhibition of protein synthesis. In addition, myostatin is able to examine the protein kinase 5' AMP-activated protein kinase (AMPK), an inhibitor of signaling of mammalian target of rapamycin (mTOR), a key regulator of protein synthesis. I populations of non-cirrhotic malnourished patients, the benefits of a therapy aimed at improving malnutrition are highlighted by reduced mortality, infections, systemic inflammatory responses and hospital stay. For cirrhotic patients, specific studies in this regard are limited by the size of the cohort and by the design of the study, and there are no effective therapies mainly due to the fact that the mechanisms of muscle loss in cirrhosis are not yet well understood. In addition, the end-points of these studies are heterogeneous (regain muscle strength, the disappearance of sarcopenia, mortality, complications of portal hypertension, etc.). In a study that used a nutritional enteral supplement, the authors observed a modest improvement in nutritional parameters but found no statistically significant differences in morbidity and mortality between the two patient groups. Better results have been reported in clinical trials that used nocturnal nutritional support, in most cases represented by branched-chain amino acids (BCAA) (leucine, isoleucine, and valine). This type of intervention has obtained an improvement in the use of energy substrates in the short term and an improvement of some nutritional parameters in the long term. Beta hydroxy-beta-methyl-butyrate (HMB) is a leucine metabolite with the potential to increase muscle performance and tropism. Studies conducted in experimental models of cachexia have reported increased phosphorylation and activation of mTOR secondary to the use of HMB. Experimental studies performed on cell cultures of myoblasts also showed an increased expression of insulin like growth factor-1 (IGF-1) secondary to treatment with HMB. These findings confirm the anabolic properties of HMB. A role of HMB on the suppression of proteolysis by inhibiting the ubiquitin-proteasome pathway in models of neoplastic cachexia and an effect on attenuating muscle atrophy secondary to steroid therapy has also been demonstrated. The association of anabolic properties targeting mTOR and anti-proteolytic make HMB a potential efficacy supplement for the treatment of sarcopenia in the cirrhotic patient. There are currently no data in the literature on the use of HMB in this category of patients. 2. Aim of the study Primary endpoints: • evaluate the effect at 3 months (T12) of HMB supplementation for 12 weeks in patients with liver cirrhosis on the mass and muscle performance compared to a group of patients The secondary objectives are: - assessment of the impact of supplementation with HMB on the development of complications (minimal and manifest hepatic encephalopathy, bacterial infections, ascites, GI bleeding) compared to the control group; - evaluation of the impact of sarcopenia on the number of admissions and the days of hospitalization for these complications compared to the control group; - identification of surrogate markers of sarcopenia through the preparation of a serum; This controlled trial is not sponsored by a drug company. 3. Patients Patients are enrolled in the study after been informed of the purpose and protocol of treatment and need to sign a written informed consent. 4. Statistical analysis, sample size, and randomization: For categorical variables, the Person-Chi-square test or the Fischer test will be used. For continuous variables, the Mann-Whitney Test will be used. The ANOVA variance analysis will be applied followed by the "t-test" when significant differences will be highlighted. Values of p <0.05 will be considered statistically significant. Block randomization, consisting of 4 individuals per block, was executed in a 1:1ratio using random numbers generated by an independent statistician (SPSS version 16.0). Knowledge of the randomization code was limited to the physician. While there is no evidence of HMB supplementation in cirrhotic patients, based on previously described variations in muscle mass in the elderly population, we aim to conduct a pilot study in which will be enrolled 20 patients per group. 5. Protocol of the study: The registration will include the main clinical and biochemical data of patients. Nutritional counseling will be provided to all patients to ensure similar caloric and daily protein intake in the two groups, according to the current guidelines (caloric intake of 20-25 kcal/kg/ die ± 10%, protein intake of 1.2 g /kg/day). At the time of the enrollment and during the subsequent controls the following data will be recorded : - calorie intake in the week preceding the visit by a three days non-consecutive food diary; - height, weight, body mass index - body composition by bioelectrical impedance analysis bioimmunoassay (BIA) - muscular function evaluated by 6-minute walk test (6MWT), Timed Up and Go test-TUGT), e l'Hand grip Test (HG). - biochemical and metabolic parameters - pharmacological therapy During the study, clinical complications (hospital admissions, infectious events, the onset of comorbidity) will be recorded. During the study, the tolerability of the supplement (analog-visual scale), adherence to absorption and any intolerance or secondary adverse effects will be detected. Randomization: Patients are randomized to Group 1 - placebo (or control group) and Group 2 - supplementation (or treatment group) The placebo will be 1gr mannitol twice daily. Supplementation will be HMB 1.5 g dissolved twice daily. Supplementation/placebo will be provided for 12 weeks.

Overall Status Unknown status
Start Date 2019-03-01
Completion Date 2020-09-10
Primary Completion Date 2019-12-10
Phase N/A
Study Type Interventional
Primary Outcome
Measure Time Frame
Changes in Fat Free Mass Index after 12 weeks of supplementation 12 weeks after the enrollment
Secondary Outcome
Measure Time Frame
Changes in Fat Free Mass Index after 24 weeks of supplementation 24 weeks after the enrollment
Changes in HG Test at 12 and 24 weeks after enrollment 12 and 24 weeks after enrollment
Changes in 6MWT Test at 12 and 24 weeks after enrollment 12 and 24 weeks after enrollment
Evaluation og Animal Naming and The Psychometric Hepatic Encephalopathy Score (PHES) 12 and 24 weeks after enrollment
Evaluation of hospitalization and decompensation episodes 12 and 24 weeks after enrollment
Enrollment 40
Condition
Intervention

Intervention Type: Dietary Supplement

Intervention Name: BETA-HYDROXY-BETA-METHYLBUTYRATE (HMB)

Description: 1.5 g of HMB will be provided for 12 weeks twice daily

Arm Group Label: HMB GROUP

Intervention Type: Dietary Supplement

Intervention Name: Mannitol

Description: 1.5 g twice daily for 12 weeks

Arm Group Label: PLACEBO GROUP

Eligibility

Criteria:

Inclusion Criteria: - all cirrhotics followed in outpatients' clinic 18-70 years Exclusion Criteria: - hepatocellular carcinoma or other neoplastic diseases; - neuromuscular or skeletal diseases, - chronic renal failure II-III degree; - cardiac decompensation with New York Heart Association (NYHA) score ≥ III; - severe pulmonary dysfunction; - active alcohol intake in the last 6 months; - ascites grade moderate-severe

Gender:

All

Minimum Age:

18 Years

Maximum Age:

70 Years

Healthy Volunteers:

No

Location
Facility: Status: Contact: Gastroenterology Department, Sapienza University of Rome Manuela Merli +39 06 49972002 [email protected]
Location Countries

Italy

Verification Date

2019-03-01

Responsible Party

Type: Principal Investigator

Investigator Affiliation: University of Roma La Sapienza

Investigator Full Name: Manuela Merli

Investigator Title: Associate Professor

Keywords
Has Expanded Access No
Condition Browse
Number Of Arms 2
Arm Group

Label: HMB GROUP

Type: Experimental

Description: HMB Supplementation with 1.5 g of HMB taken twice daily. Supplementation will be provided for 12 weeks

Label: PLACEBO GROUP

Type: Placebo Comparator

Description: Mannitol 1.5 g twice daily. Supplementation will be provided for 12 weeks

Acronym HMB
Study Design Info

Allocation: Randomized

Intervention Model: Parallel Assignment

Intervention Model Description: Parallel Assignment

Primary Purpose: Treatment

Masking: Double (Participant, Investigator)

Masking Description: patients do not know if they are placebo or HMB group. Investigators who perform measurement and patients visit do not know the group of patients

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