- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT02707042
Microbial, Immune, and Metabolic Perturbations by Antibiotics (MIME Study)
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Study Type
Enrollment (Estimated)
Phase
- Phase 1
Contacts and Locations
Study Contact
- Name: Christa S Zerbe, M.D.
- Phone Number: (301) 594-5932
- Email: zerbech@niaid.nih.gov
Study Contact Backup
- Name: Lurline Wu, C.R.N.P.
- Phone Number: (240) 550-4873
- Email: lurline.wu@nih.gov
Study Locations
-
-
Maryland
-
Bethesda, Maryland, United States, 20892
- Recruiting
- National Institutes of Health Clinical Center
-
Contact:
- For more information at the NIH Clinical Center contact Office of Patient Recruitment (OPR)
- Phone Number: TTY dial 711 800-411-1222
- Email: ccopr@nih.gov
-
-
Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Description
- SUBJECT INCLUSION CRITERIA:
Healthy women and men will be eligible for study participation if they meet the following criteria:
- A participant will have passed his/her 18th birthday and will not have attained the age of 50 at the time of enrollment.
- Willing to allow storage of their biological samples.
- Able to comply with study procedures and swallow capsules.
SUBJECT EXCLUSION CRITERIA:
An individual who meets any of the following criteria will be excluded from study participation:
- Body Mass Index (BMI) greater than or equal to 35 or less than or equal to 18 kg/M(2).
- Vital signs outside of acceptable range at Screening Visit, i.e., blood pressure >160/100, oral temperature >100 degrees F, pulse >100.
Use of any of the following drugs or devices within the last 6 months:
- systemic antibiotics, antifungals, antivirals, or antiparasitics (intravenous, intramuscular, or oral);
- oral, intravenous, intramuscular, nasal, or inhaled corticosteroids;
- cytokines;
- methotrexate or immunosuppressive cytotoxic agents;
- large doses of commercial probiotics consumed (greater than or equal to 10(8) cfu or organisms per day), including tablets, capsules, lozenges, chewing gum, or powders in which probiotic is a primary component. Ordinary dietary components such as fermented beverages/milks, yogurts, and foods do not apply.
- anabolic steroids;
- intrauterine device, combination hormone vaginal ring for contraception (due to unknown duration of local hormone effects), topical or systemic estrogens. Oral contraceptives with a standard 28-day cycle will be permitted if the subject has been consistently taking them for at least 1 month;
- oral, topical, intramuscular testosterone preparations.
- Illicit drug use, including amphetamines, cocaine, or heroin, within the last 6 months. Marijuana use is not exclusionary.
- Chronic smokers and subjects who use smokeless tobacco products (due to known effects of tobacco on the oral microbiome).
- Claustrophobia.
- Use of antacids (proton pump inhibitors, sucralfate, H1 and H2 antagonists, and those containing aluminum magnesium) within the last 3 months.
- Use of laxatives or enemas within the last 3 months.
- Diagnostic colonoscopy within the last 6 months.
- Use of topical antibiotics or topical steroids on the face, scalp, or neck, or on arms, forearms, or hands within the previous 30 days.
- Use of vaginal/vulvar medications, including antifungals, within the previous 30 days. Subjects may continue to use permitted vaginal contraceptives (spermicides and female condoms) until 24 hours prior to sampling
- Use of isotretinoin within the past 5 years.
- Intranasal influenza vaccination within the last 6 months due to effects on mucosal immunity.
- Acute disease at the time of enrollment (defer enrollment until subject recovers). Acute disease is defined as the presence of a moderate or severe illness with or without fever.
Chronic, clinically significant (unresolved, requiring ongoing medical management or medication) pulmonary, cardiovascular, dermatologic, endocrine, GI, hepatic, or renal functional abnormality, as determined by medical history, physical examination, and/or laboratory testing. Includes, but not limited to:
- A history of diabetes mellitus (Type 1 or 2), pituitary disease, hypothyroidism, hyperthyroidism
- A history of physician-diagnosed asthma
- A history of allergy to any antibiotic medications, including amoxicillin (penicillin) and/or azithromycin (macrolide)
- A history of food allergy requiring dietary accommodation
- Lactose-intolerance requiring dietary accommodation
- A history of a bleeding disorder
- Mononucleosis
- Liver disease, including non-alcoholic fatty liver disease, AST or ALT > 1.5 times normal value, cirrhosis
- Renal disease, as defined by serum creatinine concentrations > 1.5 mg/dL and/or overt proteinuria
- Central nervous system disease, including previous history of cerebrovascular accidents, dementia, and neurodegenerative disorders
- Clinically significant abnormal results on electrocardiogram (ECG) that in the opinion of the PI, would place the patient at increased risk of QT-prolongation or other cardiac event
Genitourinary/Gynecologic conditions, including:
- Treatment for or suspicion of ever having had toxic shock syndrome
- History of hysterectomy or oophorectomy
- History of condyloma or human papillomavirus diagnosed within the previous 2 years
- History of candidiasis, urinary tract infection, or sexually transmitted disease (specifically chlamydia, gonorrhea, syphilis, genital herpes, trichomoniasis) diagnosed within the previous 6 months
- Evidence (by history or physical exam) of vulvar or vaginal irritation at screening
- History of vulvar, vaginal, or cervical dysplasia within the previous 5 years
- History of cancer except for squamous or basal cell carcinomas of the skin that have been medically managed by local excision.
- Unstable dietary history as defined by major changes in diet during the previous month, where the subject has eliminated or significantly increased a major food group in the diet.
- Recent history of excessive alcohol consumption defined as more than five 1.5-ounce servings of 80-proof distilled spirits, five 12-ounce servings of beer, or five 5-ounce servings of wine at one sitting over the last 30 days.
- Positive test for HIV, hepatitis B virus, or hepatitis C virus indicating infection (hepatitis B seropositivity conferred by vaccination is not exclusionary).
- Any confirmed or suspected condition/state of immunosuppression or immunodeficiency (primary or acquired).
- Major surgery of the GI tract, including cholecystectomy or appendectomy, in the past 5 years. Any major bowel resection at any time.
- History of gastric stapling, lap band, or surgical procedure for treatment of obesity.
History of GI disorders or diseases including:
- inflammatory bowel disease (IBD) including ulcerative colitis, Crohn s disease (of any severity), or indeterminate colitis;
- irritable bowel syndrome (IBS);
- persistent, infectious gastroenteritis, colitis or gastritis, persistent or chronic diarrhea of unknown etiology, Clostridium difficile infection (recurrent), gastric or duodenal ulcer;
- Celiac disease;
- chronic constipation.
- Active behavioral or psychiatric conditions that would be incompatible with a safe and successful participation in the study, including major depression, anxiety disorder, schizophrenia, and presence of psychotic symptoms.
- Active eating disorders, including anorexia nervosa, bulimia, or binge eating syndrome.
- Use of weight-loss drugs within the past 5 years.
- Weight change (intended or unintended; loss or gain) of more than 10% of total body weight in the 3 months before admission.
- Regular urinary incontinence necessitating use of incontinence protection garments.
- Female who is pregnant, intending to become pregnant, or lactating.
- History of recurrent rashes within the past 6 months.
At the time of the screening visit:
- multiple blisters, pustules, boils, abscesses, erosions or ulcers on the scalp, face, neck, arms, forearms, or hands;
- uniformly thickened, cracking, dry skin on bilateral palms and/or soles;
- disseminated rash (at multiple body sites or extending throughout a broad body area).
- Subjects who are unable to complete required study visits per allotted visit windows.
- Any condition that, in the opinion of the investigator, contraindicates participation in this study.
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: Randomized
- Interventional Model: Parallel Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Other: Group A
Control
|
A group of volunteers will receive no antibiotics and will serve as study controls.
|
Other: Group B
Amoxicillin
|
7-day therapeutic oral course of twice daily amoxicillin
|
Other: Group C
Azithromycin
|
5-day oral course of once-daily azithromycin
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
To determine whether antibiotic-induced perturbation of the microbiome has measurable metabolic and immunologic effects during and after the treatment period.
Time Frame: Prior to, during, after antibiotic course
|
1. Change in total EE of 5% from pre-treatment to post-treatment among the subjects receiving antibiotics (metabolic endpoint).
2. Average decrease of 500 cell/mm3 in the peripheral blood leukocyte count from pre-treatment to post-treatment among subjects receiving antibiotics (immunologic endpoint).
|
Prior to, during, after antibiotic course
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Changes in parameters of metabolic functioning, including measures of hormones relevant to metabolism.
Time Frame: Prior to, during, after antibiotic course
|
Changes in 24-hr EE, its components (sleeping, diet-induced, and activity EE), macronutrient oxidation rates (carbohydrate, fat, and protein), core body temperature (to evaluate circadian rhythm), and heart rate variability (as a measure of sympathetic versus parasympathetic nervous system activities).
|
Prior to, during, after antibiotic course
|
Changes in blood, cutaneous, intestinal, oral, salivary, urinary, vaginal bacterial microbiomes;
Time Frame: Prior to, during, after antibiotic course
|
Alterations in relative abundance and function of peripheral blood cells and specialized subsets as they relate to innate and adaptive immune pathways.
|
Prior to, during, after antibiotic course
|
Changes in parameters of immune function and response in samples of blood, serum/plasma; and
Time Frame: Prior to, during, after antibiotic course
|
Changes in markers of innate and adaptive immunity as detected in serum, urine, saliva and feces, which may include immunoglobulins, cytokines, chemokines, markers of bacterial translocation and markers of systemic and mucosal inflammation.
|
Prior to, during, after antibiotic course
|
Collaborators and Investigators
Investigators
- Principal Investigator: Christa S Zerbe, M.D., National Institute of Allergy and Infectious Diseases (NIAID)
Publications and helpful links
General Publications
- McCaig LF, Besser RE, Hughes JM. Antimicrobial drug prescription in ambulatory care settings, United States, 1992-2000. Emerg Infect Dis. 2003 Apr;9(4):432-7. doi: 10.3201/eid0904.020268. Erratum In: Emerg Infect Dis. 2003 May;9(5):609.
- McCaig LF, Hughes JM. Trends in antimicrobial drug prescribing among office-based physicians in the United States. JAMA. 1995 Jan 18;273(3):214-9. Erratum In: JAMA 1998 Feb 11;279(6):434.
- Grijalva CG, Nuorti JP, Griffin MR. Antibiotic prescription rates for acute respiratory tract infections in US ambulatory settings. JAMA. 2009 Aug 19;302(7):758-66. doi: 10.1001/jama.2009.1163.
Helpful Links
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Estimated)
Study Completion (Estimated)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Estimated)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Keywords
Additional Relevant MeSH Terms
Other Study ID Numbers
- 160078
- 16-I-0078
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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