Amino Acid Kinetics in Blood After Consuming Different Milk Protein Supplements

August 24, 2016 updated by: Havard Hamarsland, Norwegian School of Sport Sciences

Does Native Whey Induce Greater Blood Leucine Concentrations Than Other Whey Protein Supplements and Milk: A Randomized Controlled Trial?

The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other milk protein supplements.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of leucine in native whey.

The aim of this double-blinded randomized 5-arm cross-over study is to compare amino acid kinetics in blood after a bout of strength training and ingestion of 20 grams of high quality, but distinct, dairy protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk). Furthermore, the investigators investigate whether differences in amino acid kinetics affect acute blood glucose and urea response, as well as recovery of muscle function after a bout of strength training.

The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other protein supplements.

Study Type

Interventional

Enrollment (Actual)

13

Phase

  • Not Applicable

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 45 years (Adult)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

Male

Description

Inclusion Criteria:

  • Healthy in the sense that they can conduct training and testing
  • Able to understand Norwegian language written and oral

Exclusion Criteria:

  • Diseases or injuries contraindicating participation
  • Use of dietary supplements (e.g. proteins, vitamins and creatine)
  • Lactose intolerance
  • Allergy to milk
  • Allergy towards local anesthetics (xylocain)

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: Randomized
  • Interventional Model: Crossover Assignment
  • Masking: Quadruple

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Placebo Comparator: Milk 1%
Participants performed a bout of strength training and consumed 636 ml of a protein supplement
Other: Strength Training
Dietary supplement: Milk 1%
Other Names:
  • Protein supplementation
Experimental: Whey protein concentrate 80 (WPC-80)
Participants performed a bout of strength training and consumed 636 ml of a protein supplement
Other: Strength Training
Dietary supplement: Whey protein concentrate 80 (WPC-80)
Other Names:
  • Protein supplementation
Experimental: Microparticulated whey
Participants performed a bout of strength training and consumed 636 ml of a protein supplement
Other: Strength Training
Dietary supplement: Microparticulated whey
Other Names:
  • Protein supplementation
Experimental: Hydrolyzed whey
Participants performed a bout of strength training and consumed 636 ml of a protein supplement
Other: Strength Training
Dietary supplement: Hydrolyzed whey
Other Names:
  • Protein supplementation
Experimental: Native whey
Participants performed a bout of strength training and consumed 636 ml of a protein supplement
Other: Strength Training
Dietary supplement: Native whey
Other Names:
  • Protein supplementation

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Time Frame
Plasma amino acid concentration change from baseline
Time Frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Serum glucose change from baseline
Time Frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Serum urea change from baseline
Time Frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle force generating capacity change from baseline
Time Frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Measured as unilateral isometric knee extension force (Nm) with 90° in the hip and knee joints.
Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Jump height change from baseline
Time Frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Measured as counter movement jump on a force plate
Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Serum creatine kinase change from baseline
Time Frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle soreness change from baseline
Time Frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Measured for m. quadriceps and m. pectoralis major on a visual analog pain scale
Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Norwegian School of Sport Sciences

Collaborators

Arkansas Children's Hospital Research Institute
Tine

Investigators

  • Principal Investigator: Truls Raastad, PhD, Norwegian School of Sport Sciences

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

September 1, 2012

Primary Completion (Actual)

October 1, 2012

Study Completion (Actual)

February 1, 2013

Study Registration Dates

First Submitted

August 16, 2016

First Submitted That Met QC Criteria

August 24, 2016

First Posted (Estimate)

August 29, 2016

Study Record Updates

Last Update Posted (Estimate)

August 29, 2016

Last Update Submitted That Met QC Criteria

August 24, 2016

Last Verified

August 1, 2016

More Information

Terms related to this study

Other Study ID Numbers

  • NorwegianSSS

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

UNDECIDED

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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