Photobiomodulation Effects on Fatigue and Muscle Damage Induced by NMES
2026年8月24日 更新者：Marco Aurélio Vaz, PhD、Federal University of Rio Grande do Sul
Effects of Photobiomodulation on Fatigue and Muscle Damage Induced by Neuromuscular Electrical Stimulation in Healthy Individuals: a Randomized Clinical Trial
Neuromuscular electrical stimulation (NMES) is widely used to improve muscle strength and mitigate atrophy.
However, its clinical efficacy is often limited by early neuromuscular fatigue, muscle damage, and discomfort, which reduce patient tolerance and contraction time.
Photobiomodulation (PBM) has emerged as a potential therapy to enhance muscle performance and accelerate recovery by minimizing fatigue and damage.
Despite its potential, few studies have investigated the effects of PBM applied specifically before an NMES-evoked fatigue protocol.
This randomized, double-blind, placebo-controlled trial aims to evaluate the acute effects of PBM on neuromuscular fatigue, muscle damage, and functional outcomes in healthy young subjects.
Participants will receive either active PBM (cluster probe: 5 lasers 810nm/200mW; 4 LEDs 660nm/10mW; Total Energy: 249.6 J per limb) or sham treatment 5 minutes before a fatiguing NMES protocol (80 contractions at 20% MVIC, 80 Hz, 1ms pulse width) applied to the quadriceps muscle.
Assessments will be performed at baseline, immediately post-intervention, and at 24h, 48h, and 72h post-intervention.
Primary outcomes include maximum and evoked isometric strength, mechanical work, and electrical muscle activity.
Secondary outcomes include muscle damage (ultrasound echo-intensity), delayed onset muscle soreness (DOMS), functional performance (unilateral vertical jump), and clinical discomfort.
The study seeks to determine if PBM can effectively modulate NMES-induced fatigue and muscle damage, potentially optimizing its clinical application.
調査の概要
状態
募集
研究の種類
介入
入学 (推定)
36
段階
- 適用できない
連絡先と場所
このセクションには、調査を実施する担当者の連絡先の詳細と、この調査が実施されている場所に関する情報が記載されています。
研究連絡先
- 名前：Marco A Vaz, PhD
- 電話番号：+555133085853
- メール：marco.vaz@ufrgs.br
研究場所
-
-
Rio Grande do Sul
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Porto Alegre、Rio Grande do Sul、ブラジル、90690200
- 募集
- Laboratório de Pesquisa do Exercício
-
コンタクト：
- Jeam M Geremia, PhD
- 電話番号：5133085853
- メール：jeam.geremia@ufrgs.br
-
主任研究者：
- Marco A Vaz, PhD
-
副調査官：
- Gustavo N Petter, MSc
-
-
参加基準
研究者は、適格基準と呼ばれる特定の説明に適合する人を探します。これらの基準のいくつかの例は、人の一般的な健康状態または以前の治療です。
適格基準
就学可能な年齢
- 大人
健康ボランティアの受け入れ
はい
説明
Inclusion Criteria:
- Healthy male and female volunteers.
- Age between 18 and 45 years.
- Practice physical activity regularly
- No history of musculoskeletal injuries in the lower limbs in the last 6 months
- Ability to tolerate NMES
Exclusion Criteria:
- Have sensory intolerable changes due to NMES
- Consumed coffee or another stimulant in the last 12 hours
- Have a body mass index (BMI) greater than 30 kg/m2 (grade I obesity)
- Present any contraindication for the performance of maximum effort
- Abnormal increase in blood pressure after maximal exertion
研究計画
このセクションでは、研究がどのように設計され、研究が何を測定しているかなど、研究計画の詳細を提供します。
研究はどのように設計されていますか？
デザインの詳細
- 主な目的：処理
- 割り当て：ランダム化
- 介入モデル：並列代入
- マスキング：トリプル
武器と介入
参加者グループ / アーム |
介入・治療 |
|---|---|
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実験的：Photobiomodulation (PBM)
Participants in this arm will receive active photobiomodulation therapy.
|
Application of PBMT using a cluster probe (Thor-DD2; 5 laser diodes: 810nm, 200mW; 4 LEDs: 660nm, 10mW).
The treatment involves 8 points on the quadriceps (2 on vastus medialis, 3 on vastus lateralis, and 3 on rectus femoris).
Each point is irradiated for 30 seconds in continuous mode with direct contact and slight pressure.
Total energy: 31.2
J per point and 249.6 J per limb.
Both therapist and participant will wear protective goggles.
Neuromuscular Electrical Stimulation (Myomed 932) applied to the quadriceps.
Parameters: 80 Hz frequency, 1 ms pulse width (biphasic rectangular pulse), 5s:10s ON:OFF ratio with 1s ramp-up/down.
The protocol lasts 20 minutes, totaling 80 evoked isometric contractions.
Current intensity is adjusted to generate an initial torque of 20% of the baseline Maximum Voluntary Isometric Contraction (MVIC).
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偽コンパレータ：Sham PBM
Participants in this arm will receive an inactive (placebo) photobiomodulation treatment.
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Neuromuscular Electrical Stimulation (Myomed 932) applied to the quadriceps.
Parameters: 80 Hz frequency, 1 ms pulse width (biphasic rectangular pulse), 5s:10s ON:OFF ratio with 1s ramp-up/down.
The protocol lasts 20 minutes, totaling 80 evoked isometric contractions.
Current intensity is adjusted to generate an initial torque of 20% of the baseline Maximum Voluntary Isometric Contraction (MVIC).
The procedure is identical to the active PBMT (8 points, 30 seconds per point, same pressure and positioning), but the device will be turned off.
To ensure blinding, participants will wear protective goggles and a blindfold to prevent visual perception of the light.
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この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Maximal voluntary isometric contraction (MVIC)
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
Largest knee extensor torque generated isometrically and voluntarily on an isokinetic dynamometer. Unit of Measure: Newton-meters (N.m) |
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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Evoked fatigue
時間枠：Continuously during the 20-minute fatigue protocol.
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Fall in the evoked torque during the fatigue protocol.
Unit of Measure: Newton-meters (N.m)
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Continuously during the 20-minute fatigue protocol.
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二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Muscle Echo Intensity
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
Quantitative analysis of muscle ultrasound images to assess changes in echo intensity, reflecting acute edema and changes in muscle quality. Analysis will be performed specifically on the Rectus Femoris (RF), Vastus Medialis (VM), and Vastus Lateralis (VL). Unit of Measure: Arbitrary Units (au) on a 0-255 grayscale |
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
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Pressure Pain Threshold
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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The minimum pressure at which a subject perceives a stimulus as painful, measured via pressure algometry on the quadriceps muscle bellies to quantify delayed onset muscle soreness (DOMS).
Unit of Measure: Newtons (N)
|
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
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Quadriceps Muscle Thickness
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
Ultrasound assessment of structural changes in the quadriceps muscle group, for the Rectus Femoris (RF), Vastus Medialis (VM), and Vastus Lateralis (VL). This variable evaluates acute morphological changes or edema-induced swelling following the NMES protocol. Unit of Measure: Millimeters (mm) |
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
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Countermovement Jump (CMJ) Height
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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Measure Description: Maximum jump height achieved during a vertical countermovement jump on a force platform to assess dynamic muscle power. Unit of Measure: Centimeters (cm) |
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
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Lower Limb Joint Kinematics During Jump
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
Measure Description: Sagittal plane kinematic analysis for the ankle, knee, hip, and trunk joints to evaluate movement strategies and joint range of motion during the countermovement jump. Unit of Measure: Degrees (°) |
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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Force-Time Curve Dynamics During Jump
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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Analysis of the vertical ground reaction force-time curve during the propulsion phase of the countermovement jump.
The primary parameter will be the Vertical Impulse, calculated as the integral of force over time.
Unit of Measure: Newton-seconds (N.s)
|
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
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Muscle Activation
時間枠：Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
Surface electromyography (sEMG) to assess muscle activation amplitude during MVIC.
Unit of Measure: Microvolts (uV)
|
Baseline (pre-intervention) on Day 1; immediately after the fatigue protocol on Day 1; and at 24, 48, and 72 hours after the fatigue protocol (on Days 2, 3, and 4, respectively).
|
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Vastus Lateralis Fascicle Length
時間枠：Continuously during the 20-minute fatigue protocol on Day 1.
|
Measure Description: Assessment of real-time changes in the fascicle length of the Vastus Lateralis muscle during the fatigue protocol using continuous ultrasound imaging. Unit of Measure: Millimeters (mm) |
Continuously during the 20-minute fatigue protocol on Day 1.
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その他の成果指標
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
|
Vastus Lateralis Pennation Angle
時間枠：Continuously during the 20-minute fatigue protocol on Day 1.
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Measure Description: Assessment of real-time changes in the pennation angle of the Vastus Lateralis muscle during the fatigue protocol using continuous ultrasound imaging. Unit of Measure: Degrees (°) |
Continuously during the 20-minute fatigue protocol on Day 1.
|
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Adipose Tissue Thickness
時間枠：Baseline
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Measurement of the fat layer thickness overlying the Rectus Femoris, Vastus Medialis, and Vastus Lateralis using ultrasound imaging.
This measure is used to characterize the sample's morphology and will serve as a covariate to adjust the delivered PBM dose and NMES current penetration analysis.
Unit of Measure: Millimeters (mm)
|
Baseline
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協力者と研究者
ここでは、この調査に関係する人々や組織を見つけることができます。
捜査官
- スタディチェア：Marco A Vaz, PhD、Federal University of Rio Grande do Sul
- スタディディレクター：Marco A Vaz, PhD、Federal University of Rio Grande do Sul
- 主任研究者：Marco A Vaz, PhD、Federal University of Rio Grande do Sul
研究記録日
これらの日付は、ClinicalTrials.gov への研究記録と要約結果の提出の進捗状況を追跡します。研究記録と報告された結果は、国立医学図書館 (NLM) によって審査され、公開 Web サイトに掲載される前に、特定の品質管理基準を満たしていることが確認されます。
主要日程の研究
研究開始 (実際)
2026年6月1日
一次修了 (推定)
2026年8月30日
研究の完了 (推定)
2026年9月30日
試験登録日
最初に提出
2026年4月27日
QC基準を満たした最初の提出物
2026年5月15日
最初の投稿 (実際)
2026年5月22日
学習記録の更新
投稿された最後の更新 (実際)
2026年8月25日
QC基準を満たした最後の更新が送信されました
2026年8月24日
最終確認日
2026年8月1日
詳しくは
本研究に関する用語
キーワード
追加の関連 MeSH 用語
その他の研究ID番号
- 5.309.992(1)
個々の参加者データ (IPD) の計画
個々の参加者データ (IPD) を共有する予定はありますか?
いいえ
IPD プランの説明
A final decision regarding the sharing of individual participant data has not yet been made.
The study team intends to evaluate this possibility following the primary data analysis, in accordance with institutional guidelines and ethical committee requirements.
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米国FDA規制医薬品の研究
いいえ
米国FDA規制機器製品の研究
いいえ
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