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Expression of TSLP Isoforms in Asthma

2026年8月24日 更新者：Medical University of Warsaw

TSLP and Its Isoforms (Short and Long Form TSLP) in Different Asthma Phenotypes

Asthma is a heterogeneous disease characterized by chronic airway inflammation, leading to variable airflow obstruction, bronchial hyperresponsiveness, excessive mucus secretion, and, consequently, structural airway remodeling. Asthma is defined by the presence of symptoms such as wheezing, dyspnea, chest tightness, and cough, with variable intensity and frequency.

Thymic stromal lymphopoietin (TSLP) is a cytokine produced primarily by epithelial cells in the lungs. Two distinct isoforms of TSLP have been identified: a long form (lfTSLP) and a short form (sfTSLP). The long isoform is induced during inflammatory conditions and promotes a T2-dependent immune response. In contrast, the short isoform is believed to exert homeostatic and anti-inflammatory functions and exhibits antimicrobial properties.

Studies have demonstrated an association between elevated serum and airway TSLP levels and increased disease severity, as well as reduced spirometric parameters. However, the literature contains limited data regarding the expression of TSLP isoforms across different asthma phenotypes and their relationship with the degree of disease control.

The goal of this study is:

  1. To assess the expression of TSLP protein and TSLP mRNA, including its isoforms (sfTSLP and lfTSLP), in serum and in airway-derived samples (nasal epithelial cells),
  2. To compare these levels among patients with different asthma phenotypes (allergic and non-allergic asthma; eosinophilic, neutrophilic, and paucigranulocytic asthma; early-onset and late-onset asthma; obesity-associated asthma; cough-variant asthma), as well as between patients with asthma and healthy controls.
  3. To analyze the correlations between the expression of TSLP isoforms (sfTSLP and lfTSLP) and asthma severity, level of disease control (as measured by the Asthma Control Questionnaire [ACQ]), pulmonary function parameters, blood eosinophil count and other clinical parameters.

調査の概要

状態

募集

条件

介入・治療

研究の種類

観察的

入学 (推定)

70

連絡先と場所

このセクションには、調査を実施する担当者の連絡先の詳細と、この調査が実施されている場所に関する情報が記載されています。

研究連絡先

研究場所

  • ポーランド
    • Warsaw
      • Warsaw、Warsaw、ポーランド、02-097
        • 募集
        • Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Warsaw, Poland,
        • コンタクト：
        • 主任研究者：
          • Marta Dąbrowska, MD, PhD, Associate Professor
        • 副調査官：
          • Izabela Orzołek, MD

参加基準

研究者は、適格基準と呼ばれる特定の説明に適合する人を探します。これらの基準のいくつかの例は、人の一般的な健康状態または以前の治療です。

適格基準

就学可能な年齢

  • 大人
  • 高齢者

健康ボランティアの受け入れ

はい

サンプリング方法

非確率サンプル

調査対象母集団

Patients with severe asthma, with different asthma phenotypes (allergic and non-allergic asthma; eosinophilic, neutrophilic, and paucigranulocytic asthma; early-onset and late-onset asthma; obesity-associated asthma; cough-variant asthma).

説明

  1. Inclusion criteria

    • age > 18 years, without the upper age limit,
    • asthma (diagnosis in accordance with the GINA 2023 guidelines),

  2. Exclusion criteria

    • other respiratory diseases (e.g., chronic obstructive pulmonary disease [COPD]);
    • active cigarette smoking or a smoking history of >10 pack-years;
    • active malignancy;
    • active pulmonary tuberculosis or active respiratory tract infection;
    • use of antibiotics within 4 weeks prior to study enrollment;
    • current long-term home oxygen therapy (>15 hours per day);
    • current treatment with monoclonal antibody therapies (e.g., omalizumab, mepolizumab, reslizumab, dupilumab, tezepelumab, or other biologics);
    • pregnancy.

研究計画

このセクションでは、研究がどのように設計され、研究が何を測定しているかなど、研究計画の詳細を提供します。

研究はどのように設計されていますか？

デザインの詳細

コホートと介入

グループ/コホート
介入・治療
Study group consists of patients with severe asthma (diagnosed according to GINA guidelines)
  1. Inclusion criteria Adults aged 18 years, without the upper age limit, diagnosed with asthma established in accordance with the GINA 2023 guidelines.
  2. Exclusion criteria Presence of other concomitant respiratory diseases (e.g., chronic obstructive pulmonary disease [COPD]); Current use of systemic glucocorticoids or immunosuppressive therapy, or use within 4 weeks prior to study enrollment; Active cigarette smoking or a smoking history of >10 pack-years; Active malignancy; Active pulmonary tuberculosis or active respiratory tract infection; Use of antibiotics within 4 weeks prior to study enrollment; Current long-term home oxygen therapy (>15 hours per day); Current treatment with monoclonal antibody therapies (e.g., omalizumab, mepolizumab, reslizumab, dupilumab, tezepelumab, or other biologics); Pregnancy.
診断テスト：No Intervention: Observational Cohort
This study is designed as a cross-sectional, observational investigation. No therapeutic or diagnostic interventions beyond routine clinical care will be introduced as part of the study. All procedures performed are limited to standard, minimally invasive sample collection and data acquisition for research purposes. Clinical management of participants will remain unchanged and will be conducted entirely at the discretion of the treating physicians. Participation in the study will not influence treatment decisions or patient care.
Control group
  1. Inclusion criteria Age above 18 years old and without the upper age limit. Negative medical history for asthma and allergic diseases.
  2. Exclusion criteria Diagnosed asthma or any other respiratory disease; Atopic dermatitis, allergic rhinitis, or other allergic diseases; Autoimmune diseases; All other exclusion criteria identical to those applied to the asthma patient group.
診断テスト：No Intervention: Observational Cohort
This study is designed as a cross-sectional, observational investigation. No therapeutic or diagnostic interventions beyond routine clinical care will be introduced as part of the study. All procedures performed are limited to standard, minimally invasive sample collection and data acquisition for research purposes. Clinical management of participants will remain unchanged and will be conducted entirely at the discretion of the treating physicians. Participation in the study will not influence treatment decisions or patient care.

この研究は何を測定していますか？

主要な結果の測定

結果測定
メジャーの説明
時間枠
TSLP mRNA expression, including the sfTSLP and lfTSLP isoforms in nasal epithelial cells and TSLP protein expression in blood and nasal epithelial cells
時間枠：This is cross-sectional studies; blood and nasal epithelial cells (nasal swab) will be taken from each study participant at one study point - through study completion, an average of 2 years

The primary endpoint of the study is the quantitative assessment of TSLP protein expression and TSLP mRNA expression, including the sfTSLP and lfTSLP isoforms, in biological material obtained from serum and nasal epithelial cells.

Peripheral venous blood samples will be collected and centrifuged to obtain serum, which will be aliquoted and stored at -80°C until analysis. Nasal epithelial cells will be collected using sterile cytology brushes. All biological samples will be processed promptly and stored under appropriate conditions prior to laboratory analysis.

TSLP protein concentrations will be measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits in accordance with the manufacturer's instructions. Measurements will be performed in duplicate, and optical density will be determined using a microplate reader. Concent
This is cross-sectional studies; blood and nasal epithelial cells (nasal swab) will be taken from each study participant at one study point - through study completion, an average of 2 years

協力者と研究者

ここでは、この調査に関係する人々や組織を見つけることができます。

スポンサー

Medical University of Warsaw

研究記録日

これらの日付は、ClinicalTrials.gov への研究記録と要約結果の提出の進捗状況を追跡します。研究記録と報告された結果は、国立医学図書館 (NLM) によって審査され、公開 Web サイトに掲載される前に、特定の品質管理基準を満たしていることが確認されます。

主要日程の研究

研究開始 (実際)

2024年4月30日

一次修了 (推定)

2026年11月1日

研究の完了 (推定)

2026年11月1日

試験登録日

最初に提出

2026年1月23日

QC基準を満たした最初の提出物

2026年8月24日

最初の投稿 (実際)

2026年8月26日

学習記録の更新

投稿された最後の更新 (実際)

2026年8月26日

QC基準を満たした最後の更新が送信されました

2026年8月24日

最終確認日

2026年1月1日

詳しくは

本研究に関する用語

キーワード

追加の関連 MeSH 用語

その他の研究ID番号

  • Medical University of Warsaw
  • 31/M/MB/N/24 (その他の助成金/資金番号：Young Scientist Grant, Medical University of Warsaw)

個々の参加者データ (IPD) の計画

個々の参加者データ (IPD) を共有する予定はありますか?

未定

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米国FDA規制医薬品の研究

いいえ

米国FDA規制機器製品の研究

いいえ

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