Normative State and Variation in Growth, Hematology, Hydration, Oxidation, Infection, Inflammation, Guatemalan Children
Study on the Normative State and Inter- and Intra-individual Variation in Growth, Hematology, Hydration and Markers of Oxidation, Infection and Inflammation in Preschool Children With a Similar Dietary Intake
This study will provide the researchers with an opportunity for the investigation of the biomarkers and different human biology component variable interactions in children; information that can generate new correlations between markers not known at the moment.
Regarding their analysis, variation could have multiple connotations. A categorical that refers to a specific state, established with a diagnostic standard. And a numerical, referring to a range or the absolute value limits in a continuous spectrum; this research will allow us to express variability in the distance amplitude of the obtained values level, as well as classifications or categories previously established in the possible cases.
OBJECTIVES PRINCIPAL OBJECTIVE
To estimate the inter and intra-individual variety in a selected series or biological variables determinant of growth, body composition, infection, oxidation, inflammation and hydration, in preschool children sharing the same institutional-based diet.
SPECIFIC OBJECTIVES
- To determine the inter and intra-individual variation of growth in preschool children, with a similar diet, who attend Secretariat of Beneficial Works of the First Lady system daycare centers and to compare between semi-urban and rural.
- To describe the inter-individual variation of the red blood cells circulating mass in preschool children, with a similar diet, who attend day care centers and to compare between semi-urban and rural.
- To determine the inter-individual variation of systemic and intraintestinal oxidation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural.
- To describe the inter-individual variation of systemic and intraintestinal inflammation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural.
- To determine the inter and/or intra-individual variation of urinary tract infection and gastrointestinal parasite infestation in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural.
- To describe the inter and intra-individual variation of the hydration status in preschool children, with a similar diet, who attend daycare centers and to compare between semi-urban and rural.
- To explore intra-individual associations in variables of the same research component or different component variables.
研究概览
地位
条件
详细说明
研究类型
注册 (实际的)
联系人和位置
学习地点
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Quetzaltenango
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Guatemala、Quetzaltenango、危地马拉、09001
- Center for Studies of Sensory Impairment Aging and Metabolism
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- To be attending one of the SOSEP systems assigned daycare centers
- To maintain an attendance of 80% during the 8 weeks of the fieldwork
- Aged 23 to 84 months
- Being apparently healthy and with no dietary restrictions related to the foods offered with the SOSEP menu.
Exclusion Criteria:
- Children whose parents of caregivers did not sign a consent form
- Children who refused to participate in the study
- Children who refused to adhere to the collections routines.
学习计划
研究是如何设计的？
设计细节
队列和干预
团体/队列 |
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Apparently Healthy
Apparently healthy preschool children who attend 3 daycare centers of the Secretariat of Beneficials Works of the First Lady in Guatemalan Western Highlands
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
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Weight
大体时间：7th week of observation
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Growth, measured trough weight (kg)
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7th week of observation
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Hemoglobin
大体时间：8th week of observation
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Hemoglobin (mg/dl) measured in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango, Guatemala.
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8th week of observation
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Urine Osmolality-1
大体时间：6th week of observation
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Hydration Status= measured in 24-h urine through urine volume and osmolality in osmometer Model Löser 815.
Results in mOsm/kg.
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6th week of observation
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Height
大体时间：7th week of observation
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Growth, measured trough height (cm).
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7th week of observation
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Urine Osmolality-2
大体时间：7th week of observation
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Hydration Status= measured in 24-h urine through urine volume and osmolality Osmometer Model Löser 815.
Results in mOsm/kg.
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7th week of observation
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Urine Osmolality-3
大体时间：8th week of observation
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Hydration Status= measured in 24-h urine through urine volume and osmolality in two different osmometers (Löser 815 and Osmomat 030).
Results in mOsm/kg.
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8th week of observation
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Urine Urea
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: urea (mg/l) |
8th week of oservation
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Urine Uric Acid
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: uric acid (mg/l) |
8th week of oservation
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Urine Sodium
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: sodium (mg/l) |
8th week of oservation
|
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Urine Potassium
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: potassium (mg/l) |
8th week of oservation
|
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Urine Calcium
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: calcium (mg/l) |
8th week of oservation
|
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Urine Magnesium
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: magnesium (mg/l) |
8th week of oservation
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Urine Phosphorus
大体时间：8th week of oservation
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Urine solutes measured by the Scientific Instrumentation Center from the Granada University, Spain: inorganic phosphorus (mg/l) |
8th week of oservation
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Urine 15-f2t-Isoprostanes
大体时间：8th week of observation
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Urine Oxidation biomarkers: 15-f2t-Isoprostanes (ng/mL) Measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada using ELISA assay kit (Oxford Biomedical Research, Inc., Catalog # EA84.102606, Michigan, USA) |
8th week of observation
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Urine 8-Hydroxydeoxyguanosine
大体时间：8th week of observation
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Urine Oxidation biomarkers: 8-Hydroxydeoxyguanosine (ng/mL) Measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada using ELISA assay kits (JaICA, Nikken SEIL Co., Ltd, Catalog# IM-KOGHS 040914E, Shizouka, Japan). |
8th week of observation
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Catalase Activity
大体时间：8th week of observation
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Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system: Catalase Activity (nmol/seg/g Hb) |
8th week of observation
|
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Superoxide Dismutase Activity
大体时间：8th week of observation
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Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Superoxide Dismutase Activity (U/g Hb)
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8th week of observation
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Gluthathione Reductase
大体时间：8th week of observation
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Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Gluthathione Reductase Activity (umol/min/g Hb)
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8th week of observation
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Gluthathione Peroxidase
大体时间：8th week of observation
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Activity of antioxidant enzymes in red blood cells; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using ELISA system Gluthathione Peroxidase Activity (uU/g Hb)
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8th week of observation
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Retinol
大体时间：8th week of observation
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Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Retinol (mg/dl) |
8th week of observation
|
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Alpha-tocopherol
大体时间：8th week of observation
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Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Alpha-tocopherol (mg/l) |
8th week of observation
|
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Beta-Carotenes
大体时间：8th week of observation
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Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Beta-carotenes (mg/l) |
8th week of observation
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Coenzyme Q9
大体时间：8th week of observation
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Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Coenzyme Q9 (mg/l) |
8th week of observation
|
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Coenzyme Q10
大体时间：8th week of observation
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Content of antioxidant compounds in plasma; Scientific Instrumentation Center, University of Granada. Coenzyme Q10 (mg/l) |
8th week of observation
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Plasma Interleukin-1B
大体时间：8th week of observation
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Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-1B (pg/ml) |
8th week of observation
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Plasma Interleukin-6
大体时间：8th week of observation
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Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-6 (pg/ml) |
8th week of observation
|
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Plasma Interleukin-8
大体时间：8th week of observation
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Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-8 (pg/ml) |
8th week of observation
|
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Plasma Interleukin-10
大体时间：8th week of observation
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Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma IL-10 (pg/ml) |
8th week of observation
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Plasma Tumor Necrosis Factor-Alpha
大体时间：8th week of observation
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Plasma Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Plasma Tumor Necrosis Factor-Alpha (pg/ml) |
8th week of observation
|
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Saliva Interleukin-1B
大体时间：8th week of observation
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Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-1B (pg/ml)
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8th week of observation
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Saliva Interleukin-6
大体时间：8th week of observation
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Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-6 (pg/ml)
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8th week of observation
|
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Saliva Interleukin-8
大体时间：8th week of observation
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Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva IL-8 (pg/ml)
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8th week of observation
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Saliva Interleukin-10
大体时间：8th week of observation
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Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system Saliva IL-10 (pg/ml)
|
8th week of observation
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Saliva Tumor Necrosis Factor-Alpha
大体时间：8th week of observation
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Saliva Interleukines; measured at the Institute of Nutrition and Food Technology, Center of Biomedical Research, University of Granada, using the Luminex system: Saliva Tumor Necrosis Factor-Alpha (pg/ml) |
8th week of observation
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Fecal Calprotectin
大体时间：8th week of observation
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Fecal Calprotectin (mg/kg) measured using the ELISA assay kit from Calpro-Lab (Catalog # CAL0100).
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8th week of observation
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Intestinal Parasitosis
大体时间：8th week of observation
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Intestinal Parasitosis, measured by Biochemistry Laboratory, La Democracia Hospital, Quetzaltenango, Guatemala.
Parasite cysts presence in feces.
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8th week of observation
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Giardiasis Infection Presence-1
大体时间：7th week of observation
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Analyses done by ELISA assay kit (ProSpecT Giardia EZ, catalog #R2458596), absorbance (O.D)
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7th week of observation
|
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Giardiasis Infection Presence-2
大体时间：8th week of observation
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Analyses done by ELISA assay kit (ProSpecT Giardia EZ, catalog #R2458596), absorbance (O.D)
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8th week of observation
|
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White Urine Cell Count
大体时间：8th week of observation
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White urine cell count (per field), to be determined in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango Guatemala.
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8th week of observation
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White Blood Cell Count
大体时间：8th week of observation
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White blood cell count (cells/ul), to be determined in Biochemistry Laboratory in La Democracia Hospital, Quetzaltenango Guatemala.
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8th week of observation
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合作者和调查者
调查人员
- 首席研究员：Maria J. Soto-Méndez, MSc、Center for Studies of Sensory Impairment, Aging and Metabolism
出版物和有用的链接
一般刊物
- Soto-Méndez M, Campos-Oliva R, Aguilera C, Solomons N, Schümann K, Gil A. Urinary osmolality of preschool children with a largely common weekday meal offering, from the western highlands of Guatemala. Spanish J Community Nutr. 20:13-9, 2014.
- Soto-Mendez MJ, Romero-Abal ME, Schumann K, Gil A, Solomons NW. Normative Fecal Calprotectin Concentrations in Guatemalan Preschoolers Are High Relative to Children Reported Elsewhere. J Pediatr Gastroenterol Nutr. 2017 Feb;64(2):238-244. doi: 10.1097/MPG.0000000000001241.
- Soto-Mendez MJ, Aguilera CM, Campana-Martin L, Martin-Laguna V, Schumann K, Solomons NW, Gil A. Variation in hydration status within the normative range is associated with urinary biomarkers of systemic oxidative stress in Guatemalan preschool children. Am J Clin Nutr. 2015 Oct;102(4):865-72. doi: 10.3945/ajcn.114.105429. Epub 2015 Aug 12.
- Soto-Mendez MJ, Aguilera CM, Mesa MD, Campana-Martin L, Martin-Laguna V, Solomons NW, Schumann K, Gil A. Interaction of Giardia intestinalis and Systemic Oxidation in Preschool Children in the Western Highlands of Guatemala. J Pediatr Gastroenterol Nutr. 2016 Jul;63(1):118-22. doi: 10.1097/MPG.0000000000000891.
研究记录日期
研究主要日期
学习开始
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (估计)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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