North Carolina Newborn Exome Sequencing for Universal Screening (NC_NEXUS)
研究概览
地位
条件
详细说明
The investigators will enroll and perform whole exome sequencing on two cohorts of patients. One cohort will consist of two hundred newborns with no known conditions whose parents will be recruited during the mother's pregnancy. The second cohort will include two hundred infants and children up to the age of five years with diagnosed conditions including conditions detected through standard newborn screening such as phenylketonuria and other inborn errors of metabolism, hearing loss and other rare conditions that may fit criteria for newborn screening in the future.
Parents will be introduced to the study by their clinician or a study recruiter. Those who agree to enroll in Phase I will review an online decision guide and be offered a study visit conducted by a genetic counselor to obtain informed consent for genomic sequencing of their child. Parents consenting to have their child's genome sequenced will be seen after the child's birth or at a convenient pre-arranged time and duplicate saliva samples will be collected from the children and one sample will be sent to the BioSpecimen Processing (BSP) Facility and to Dr. Jonathan Berg's laboratory for sequencing and the other sent to the Molecular Genetics Laboratory (MGL) for DNA extraction and storage until needed for clinical confirmation. Results will be returned for diagnostic (in the Diagnosed cohort) and medically actionable disorders of childhood (both cohorts). Two-thirds of parents who consent to sequencing will be randomly assigned to be eligible to request additional findings and use a supplement of the online decision aid. All results will be reported to parents by trained genetic professionals (genetic counselors and clinical geneticists)
研究类型
注册 (实际的)
阶段
- 不适用
联系人和位置
学习地点
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North Carolina
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Chapel Hill、North Carolina、美国、27599
- Unc Hospitals
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- Uncomplicated pregnancy and healthy newborn
Exclusion Criteria:
- Abnormalities such as major malformation or chromosomal disorder detected prenatally or significant complications during pregnancy or at the time of delivery.
学习计划
研究是如何设计的？
设计细节
- 主要用途：诊断
- 分配：随机化
- 介入模型：并行分配
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
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其他：Well infant, whole exome sequencing
Healthy infants and their parents enrolled in the study prenatally will participate.
After the infant is born saliva sample will be collected for DNA extraction and whole exome sequencing will be done.
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Whole exome sequencing will be performed in children with diagnosed conditions.
Investigators will analyze results that are associated with their condition.
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其他：Diagnosed, whole exome sequencing
Infants and children with diagnosed conditions whose parents enroll in the study and consent to having their child sequenced will have saliva samples obtained and whole exome sequencing will be done on extracted DNA.
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In addition to returning results of conditions associated with a child's phenotype, investigators will also analyze genes that are associated with conditions that have childhood onset and are medically actionable.
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研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
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Parental Choices Following Decision Aid
大体时间：average of 3-6 months
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Analysis of parents' decisions after they complete an on-line decision aid to see if they wish to participate in the study.
Options will be yes, no, or undecided.
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average of 3-6 months
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Number of Participants Identified With Genetic Conditions Through Whole Exome Sequencing
大体时间：approximately 3-6 months after DNA sample obtained
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Investigators analyzed next generation sequencing (NGS) results in the diagnosed cohort to determine the ability of whole exome sequencing to detect pathogenic variants in genes related to phenotype determined by standard newborn screening (NBS).
The category of genes analyzed is termed the Next Generation Sequencing/Newborn Screening (NGS/NBS) category.
Healthy newborns with no known genetic conditions also had the NGS/NBS category of genes analyzed.
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approximately 3-6 months after DNA sample obtained
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
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Parental Reaction Scores
大体时间：Time 3 - 2 weeks after results visit and Time 4 - 3 months after results visit
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Test-related distress is assessed with an adapted version of the Multidimensional Impact of Cancer Risk Assessment (MICRA).
It asks participants to report how often in the past week they have experienced worries and distress related to their child's genomic sequencing procedure and test results, and the social and familial consequences of sequencing and the test results.
Possible responses are provided on the following scale: 0=Never, 1=Rarely, 3=Sometimes, and 5=Often.
Because it refers to respondents' experience of their child's sequencing and the test results they received, it is administered only in assessments that occurred after sequencing at Time 3 (2 weeks after results visit and Time 4 (3 months after results visit).
Comparisons are made between couples who could chose to receive additional information about their child's genome and a control group who were not eligible to receive additional information.
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Time 3 - 2 weeks after results visit and Time 4 - 3 months after results visit
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合作者和调查者
合作者
调查人员
- 首席研究员：Jonathan Berg, MD, PhD、University of North Carolina School of Medicine Department of Genetics
- 首席研究员：Cynthia M Powell, MD、University of North Carolina School of Medicine Department of Pediatrics
出版物和有用的链接
研究记录日期
研究主要日期
学习开始
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
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