Human Muscle Gene Expression Profiles in Young and Old Men (CERA)
To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.
The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.
The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.
연구 개요
연구 유형
등록 (실제)
연락처 및 위치
연구 장소
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Saint-etienne, 프랑스, 42000
- CHU de Saint-Etienne
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참여기준
자격 기준
공부할 수 있는 나이
건강한 자원 봉사자를 받아들입니다
연구 대상 성별
샘플링 방법
연구 인구
설명
Inclusion Criteria:
- Affiliated or beneficiary of a social security category
- Having signed the inform consent form
- Having signed the genetic consent form
Exclusion Criteria:
- Antiaggregant platelet treatment
- Type 2 diabetes
- Body Mass Index > 25
- Severe renal disease
- CRP > 10
- Abnormal clotting test
- Allergies to local anesthetics
공부 계획
연구는 어떻게 설계됩니까?
디자인 세부사항
코호트 및 개입
그룹/코호트 |
개입 / 치료 |
|---|---|
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Young
Young men 19-25 years old
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W1 - Blood test
W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy |
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Old
old men 70-76 years old, who participate in the PROOF study (NCT 00759304)
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W1 - Blood test
W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy |
연구는 무엇을 측정합니까?
주요 결과 측정
결과 측정 |
기간 |
|---|---|
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To identify the differential expression profiles (proteomics, transcriptomics) in skeletal muscle between young and old men
기간: During biopsie (W3)
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During biopsie (W3)
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2차 결과 측정
결과 측정 |
기간 |
|---|---|
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To specify the localisation of the major biomarkers in the muscle biopsies.
기간: During biopsie (W3)
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During biopsie (W3)
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To compare muscular energy metabolic enzymatic activities between young and old men
기간: during biopsie (W3)
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during biopsie (W3)
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To compare the number of muscular stem cells between young and old men
기간: during biopsie (W3)
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during biopsie (W3)
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공동 작업자 및 조사자
협력자
수사관
- 연구 의자: Daniel BECHET, PhD, INRA Clermont-Ferrand
연구 기록 날짜
연구 주요 날짜
연구 시작
기본 완료 (실제)
연구 완료 (실제)
연구 등록 날짜
최초 제출
QC 기준을 충족하는 최초 제출
처음 게시됨 (추정)
연구 기록 업데이트
마지막 업데이트 게시됨 (추정)
QC 기준을 충족하는 마지막 업데이트 제출
마지막으로 확인됨
추가 정보
이 정보는 변경 없이 clinicaltrials.gov 웹사이트에서 직접 가져온 것입니다. 귀하의 연구 세부 정보를 변경, 제거 또는 업데이트하도록 요청하는 경우 register@clinicaltrials.gov. 문의하십시오. 변경 사항이 clinicaltrials.gov에 구현되는 즉시 저희 웹사이트에도 자동으로 업데이트됩니다. .
Skeletal muscle aging에 대한 임상 시험
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Fayoum University초대로 등록
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Zagazig University완전한
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Bakirkoy Dr. Sadi Konuk Research and Training Hospital모집하지 않고 적극적으로
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Mohammed Gaber Saad모집하지 않고 적극적으로