- ICH GCP
- Amerikanska kliniska prövningsregistret
- Klinisk prövning NCT00725166
Human Muscle Gene Expression Profiles in Young and Old Men (CERA)
To reach the goals of living longer in better medical conditions, many countries reach the same conclusion: new strategies have to be developed to avoid, or at least limit, the effects of age; this requires a better knowledge of the mechanisms of aging. Our project focuses on the loss of muscle mass associated with aging, called sarcopenia. Sarcopenia unavoidably leads to impaired mobility and poor balance, which contributes to loss of functional autonomy and to increased prevalence for severe falls. Skeletal muscle also plays a central role as a reserve for energy and amino acids. Hence, sarcopenia further triggers severe side metabolic effects such as frailty among elderly persons. The precise mechanisms of muscle aging are still mostly unknown, although many theories have been proposed.
The present study aims at better understanding the mechanisms of skeletal muscle loss associated with aging. Using muscle biopsies from young and old subjects, the differential expression profiles of mRNA will be obtained through chips that will evaluate more than 39000 transcripts. On the same samples, proteomic analyses will involve two complementary approaches: (1) bidimensional electrophoresis (2DGE) coupled to mass spectrometry (MALDI-ToF) for dominant proteins; (2) Western-blot (more than 800 antibodies) targeting regulating proteins not detectable using 2DGE. Complementary histological studies (immunohisto-fluorescence, confocal microscopy) will specify the localisation of the major biomarkers in the muscle biopsies.
The results of that research will have applications in the medium term and will lead to nutritional interventions to modulate specific metabolic pathways and improve the quality of life in the elderly.
Studieöversikt
Studietyp
Inskrivning (Faktisk)
Kontakter och platser
Studieorter
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Saint-etienne, Frankrike, 42000
- CHU de Saint-Etienne
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Deltagandekriterier
Urvalskriterier
Åldrar som är berättigade till studier
Tar emot friska volontärer
Kön som är behöriga för studier
Testmetod
Studera befolkning
Beskrivning
Inclusion Criteria:
- Affiliated or beneficiary of a social security category
- Having signed the inform consent form
- Having signed the genetic consent form
Exclusion Criteria:
- Antiaggregant platelet treatment
- Type 2 diabetes
- Body Mass Index > 25
- Severe renal disease
- CRP > 10
- Abnormal clotting test
- Allergies to local anesthetics
Studieplan
Hur är studien utformad?
Designdetaljer
Kohorter och interventioner
Grupp / Kohort |
Intervention / Behandling |
---|---|
Young
Young men 19-25 years old
|
W1 - Blood test
W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy |
Old
old men 70-76 years old, who participate in the PROOF study (NCT 00759304)
|
W1 - Blood test
W2 - Maximal metabolic test using a cycloergometer W3 - Muscular biopsy |
Vad mäter studien?
Primära resultatmått
Resultatmått |
Tidsram |
---|---|
To identify the differential expression profiles (proteomics, transcriptomics) in skeletal muscle between young and old men
Tidsram: During biopsie (W3)
|
During biopsie (W3)
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Sekundära resultatmått
Resultatmått |
Tidsram |
---|---|
To specify the localisation of the major biomarkers in the muscle biopsies.
Tidsram: During biopsie (W3)
|
During biopsie (W3)
|
To compare muscular energy metabolic enzymatic activities between young and old men
Tidsram: during biopsie (W3)
|
during biopsie (W3)
|
To compare the number of muscular stem cells between young and old men
Tidsram: during biopsie (W3)
|
during biopsie (W3)
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Samarbetspartners och utredare
Samarbetspartners
Utredare
- Studiestol: Daniel BECHET, PhD, INRA Clermont-Ferrand
Studieavstämningsdatum
Studera stora datum
Studiestart
Primärt slutförande (Faktisk)
Avslutad studie (Faktisk)
Studieregistreringsdatum
Först inskickad
Först inskickad som uppfyllde QC-kriterierna
Första postat (Uppskatta)
Uppdateringar av studier
Senaste uppdatering publicerad (Uppskatta)
Senaste inskickade uppdateringen som uppfyllde QC-kriterierna
Senast verifierad
Mer information
Termer relaterade till denna studie
Nyckelord
Ytterligare relevanta MeSH-villkor
Andra studie-ID-nummer
- 0708102
- 2008-A00374-51
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