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Volume-Stable Collagen Matrix vs. Connective Tissue Graft for Peri-Implant Soft Tissue Defects

2026년 8월 21일 업데이트: Merve Atak, Ankara University

Clinical, Histological, Biomolecular, and Ultrasonographic Analysis of the Efficacy of a Volume-Stable Collagen Matrix Versus an Autogenous Connective Tissue Graft in the Treatment of Peri-Implant Soft-Tissue Defects

Peri-implant soft-tissue defects will be treated with either a volume-stable collagen matrix (VCMX; test group) or an autogenous connective tissue graft (CTG; control group) following implant placement in patients with a single missing tooth. After a mid-crestal incision and full-thickness flap elevation, the graft material will be positioned over the crest beneath the buccal flap and stabilized, followed by tension-free primary closure. Peri-implant soft-tissue thickness, echo intensity, and vascularization will be assessed non-invasively using high-resolution intraoral ultrasonography and Power Doppler imaging. Clinical periodontal parameters and patient-reported outcomes, including pain perception (visual analog scale), swelling, fatigue, analgesic consumption, and wound healing, will be recorded. These outcomes will be evaluated at baseline and on the 1st week, 1st, 3rd, and 6th months. At the 6th month, gingival biopsy samples obtained during healing abutment placement will be evaluated histologically and immunohistochemically (CD11c, CD163, COL1, COL3, MMP-1, TIMP-1) to compare the biological integration of the two graft materials.

연구 개요

상태

모병

정황

개입 / 치료

상세 설명

Adequate peri-implant soft-tissue thickness is important for both esthetic outcomes and the long-term maintenance of peri-implant health, as thin mucosa has been associated with increased marginal bone loss. Although the autogenous connective tissue graft is considered the gold standard for soft-tissue augmentation, it entails disadvantages such as the need for a second surgical site and donor-site morbidity. The volume-stable collagen matrix has been proposed as an alternative that eliminates the need for a donor site; however, comparative data based on ultrasonographic, histological, and immunohistochemical evidence remain limited. This study aims to compare VCMX and CTG using a multidimensional approach.

The study is designed as a single-center, randomized, controlled clinical trial to be conducted at the Department of Periodontology, Faculty of Dentistry, Ankara University. Participants meeting the eligibility criteria will be allocated to the test (VCMX) and control (CTG) groups. All surgical and clinical procedures will be performed according to standardized protocols, and all data will be recorded prospectively.

The evaluation will adopt a multidimensional methodological approach: the assessment of quantitative data obtained by high-resolution intraoral ultrasonography (soft-tissue thickness, vascularization, and echo intensity) together with histological and immunohistochemical findings constitutes the novel aspect of this study. The primary outcome will be the change in ultrasonographic soft-tissue thickness, whereas the secondary outcomes will comprise vascularization, echo intensity, clinical periodontal parameters, patient-reported outcomes, and the histological/immunohistochemical evaluation of graft integration. In this way, the two graft materials will be compared not only in terms of dimensional gain but also with respect to biological integration and tissue quality.

연구 유형

중재적

등록 (추정된)

22

단계

  • 해당 없음

연락처 및 위치

이 섹션에서는 연구를 수행하는 사람들의 연락처 정보와 이 연구가 수행되는 장소에 대한 정보를 제공합니다.

연구 연락처

연구 연락처 백업

연구 장소

  • 터키 (Türkiye)
    • Yeni̇mahalle
      • Ankara, Yeni̇mahalle, 터키 (Türkiye), 06560
        • 모병
        • Faculty of Dentistry, Ankara University
        • 연락하다:

참여기준

연구원은 적격성 기준이라는 특정 설명에 맞는 사람을 찾습니다. 이러한 기준의 몇 가지 예는 개인의 일반적인 건강 상태 또는 이전 치료입니다.

자격 기준

공부할 수 있는 나이

  • 성인
  • 고령자

건강한 자원 봉사자를 받아들입니다

예

설명

Inclusion Criteria:

  • Age >18 years
  • Periodontal health confirmed according to the 2017 World Workshop criteria (BOP <10%, PD ≤3 mm, absence of gingival inflammation, and good oral hygiene) (Tonetti et al., 2018)
  • Requirement of implant placement and soft-tissue augmentation for a single missing tooth in the anterior region
  • Bleeding on probing <30% at the adjacent teeth (Thoma et al., 2016)
  • Compliance with the study protocol and follow-up visits

Exclusion Criteria:

  • Siebert Class II or Class III defects
  • History of surgery at the treated site
  • Untreated periodontitis or peri-implantitis
  • Current smoking or smoking within the previous five years
  • Systemic conditions (that may impair healing)
  • Pregnancy or lactation
  • Allergy to the materials/drugs used
  • Drug use affecting mucosal healing
  • History of antibiotic therapy within the previous six months

공부 계획

이 섹션에서는 연구 설계 방법과 연구가 측정하는 내용을 포함하여 연구 계획에 대한 세부 정보를 제공합니다.

연구는 어떻게 설계됩니까?

디자인 세부사항

  • 주 목적: 치료
  • 할당: 무작위
  • 중재 모델: 병렬 할당
  • 마스킹: 더블

무기와 개입

참가자 그룹 / 팔
개입 / 치료
실험적: VCMX Group
Soft-tissue augmentation will be performed using a volume-stable collagen matrix (VCMX). Under local anesthesia, a mid-crestal incision combined with sulcular incisions will be made, and a full-thickness flap will be elevated with periosteal releasing incisions to eliminate tension. Implants will be placed according to prosthetic and surgical principles and covered with a cover screw. The VCMX (15×20×3 mm) will be trimmed to the defect size, positioned over the crest beneath the buccal flap, and stabilized with simple interrupted sutures (6-0 polypropylene). Tension-free primary flap closure will be achieved with simple interrupted sutures (5-0 polypropylene).
장치: Volume-stable collagen matrix
A volume-stable porcine-derived collagen matrix (15×20×3 mm) will be trimmed to the defect size, placed over the crest beneath the buccal flap, and stabilized with simple interrupted sutures (6-0 polypropylene) to augment peri-implant soft-tissue thickness. (test group)
활성 비교기: CTG Group
Soft-tissue augmentation will be performed using an autogenous connective tissue graft (CTG). Under local anesthesia, a mid-crestal incision combined with sulcular incisions will be made, and a full-thickness flap will be elevated with periosteal releasing incisions to eliminate tension. Implants will be placed according to prosthetic and surgical principles and covered with a cover screw. A CTG harvested using the extraoral de-epithelialization technique will be positioned over the crest beneath the buccal flap and stabilized with simple interrupted sutures (6-0 polypropylene). Tension-free primary flap closure will be achieved with simple interrupted sutures (5-0 polypropylene).
절차: Connective tissue graft
An autogenous connective tissue graft harvested from the palate using the extraoral de-epithelialization technique will be placed over the crest beneath the buccal flap and stabilized with simple interrupted sutures (6-0 polypropylene) to augment peri-implant soft-tissue thickness. (control group)

연구는 무엇을 측정합니까?

주요 결과 측정

결과 측정
측정값 설명
기간
peri-implant soft-tissue thickness
기간: Baseline, 1 week, 1 month, 3 months, and 6 months
Peri-implant buccal soft-tissue thickness will be measured non-invasively using high-resolution intraoral ultrasonography (LOGIQ P10 XDClear R4.5, GE Healthcare) with an L8-18i-RS high-frequency linear "hockey-stick" probe (8 MHz). Measurements will be repeated preoperatively (baseline) and postoperatively at 1 week, 1 month, 3 months, and 6 months. On static B-mode images, linear soft-tissue thickness will be assessed at three levels 1.5, 3, and 5 mm apical to the alveolar crest margin and recorded in millimeters by a single calibrated examiner blinded to group allocation. The primary outcome is the change in linear soft-tissue thickness from baseline to 6 months, compared between the VCMX and CTG groups.
Baseline, 1 week, 1 month, 3 months, and 6 months

2차 결과 측정

결과 측정
측정값 설명
기간
Peri-implant soft-tissue vascularization
기간: Baseline, 1 week, 1 month, 3 months, and 6 months
Peri-implant soft-tissue perfusion will be evaluated using Power Doppler ultrasonography (PDUS) with the same unit and probe, preoperatively (baseline) and postoperatively at 1 week, 1 month, 3 months, and 6 months. Quantitative analysis will use the device's integrated Color Quantification (CQ) module: a circular region of interest (ROI) 6 mm in diameter (area 28.274 mm²), originating from the alveolar crest margin, will be defined. Within this ROI, the proportion of colored (Power Doppler) pixels representing blood flow relative to the total number of pixels will be calculated to obtain a semi-quantitative Power Doppler pixel density . The outcome is the change in pixel density from baseline to 6 months, compared between the VCMX and CTG groups.
Baseline, 1 week, 1 month, 3 months, and 6 months
Ultrasonographic echo intensity of peri-implant soft tissue
기간: Baseline, 1 week, 1 month, 3 months, and 6 months
Tissue echogenicity will be evaluated preoperatively (baseline) and postoperatively at 1 week, 1 month, 3 months, and 6 months from static B-mode images obtained with the probe positioned longitudinally over the mid-buccal region of the edentulous site. Echo intensity will be measured in decibels (dB) using the unit's grayscale (echo-intensity) function. The ROI will extend 7 mm apically from the soft-tissue margin. As the device references echo intensity to the system's saturation level, soft-tissue reflections yield negative dB values; less negative values indicate higher echogenicity (denser, more organized tissue), and more negative values indicate hypoechoic/less dense tissue. Measurements will be performed by a single calibrated, blinded examiner, with intra-examiner reliability assessed beforehand. The outcome is the change in echo intensity from baseline to 6 months, compared between the VCMX and CTG groups.
Baseline, 1 week, 1 month, 3 months, and 6 months
Postoperative pain
기간: 1 week
Patient-rated pain intensity at 1 week, recorded on a Visual Analog Scale (VAS) anchored at 0 (no pain) and 10 (most severe pain). Compared between the VCMX and CTG groups.
1 week
Postoperative swelling
기간: 1 week
Patient-rated swelling at 1 week on a Visual Analog Scale (VAS) from 0 (no swelling) to 10 (most severe). Compared between the VCMX and CTG groups.
1 week
Postoperative fatigue
기간: 1 week
Patient-rated fatigue at 1 week on a Visual Analog Scale (VAS) from 0 (no fatigue) to 10 (most severe). Compared between the VCMX and CTG groups
1 week
Number of analgesic tablets consumed
기간: 1 week
Total number of analgesic tablets taken during the first postoperative week, recorded by patient self-report. Compared between the VCMX and CTG groups.
1 week
Early wound healing (Wound Healing Index, WHI)
기간: 1 week
Early wound healing at 1 week scored with the Wound Healing Index (Huang et al., 2005) based on edema, erythema, discomfort, flap dehiscence, and suppuration, from 1 (uneventful healing) to 3 (poor healing). Compared between the VCMX and CTG groups.
1 week
Soft-tissue appearance
기간: 6 months
Soft-tissue appearance at 6 months graded with the Aichelmann-Reidy et al. (2001) tissue-appearance system across five parameters (tissue consistency, contour, color match, scar formation, and integration with adjacent tissue), each scored against predefined criteria and summed into a total appearance score. Compared between the VCMX and CTG groups.
6 months
Patient satisfaction with appearance
기간: 6 months
Number of participants rating the appearance of the treated site as "satisfactory" versus "not satisfactory" at 6 months. Compared between the VCMX and CTG groups.
6 months
Patient satisfaction with experience
기간: 6 months
Number of participants rating their treatment experience as "satisfactory" versus "not satisfactory" at 6 months. Compared between the VCMX and CTG groups.
6 months
Histomorphological evaluation
기간: 6 months
Descriptive light-microscopic evaluation (hematoxylin-eosin and Masson's trichrome) of tissue architecture, integrity and cellular organization, extracellular matrix organization, and the presence of necrosis, edema, hemorrhage, inflammation, foreign-body reaction, and fibrosis.
6 months
Microvascular density
기간: 6 months
Number of vessels counted in the superficial connective tissue at x400 magnification (single field = 0.237 mm²) across three consecutive fields, averaged per specimen. Compared between the VCMX and CTG groups.
6 months
CD163
기간: 6 months
Number of CD163-positive cells counted at x400 magnification (single field = 0.237 mm²) across three hot-spot fields, averaged per specimen. Compared between the VCMX and CTG groups.
6 months
CD11c
기간: 6 months
Number of CD11c-positive cells counted at x400 magnification (single field = 0.237 mm²) across three hot-spot fields, averaged per specimen. Compared between the VCMX and CTG groups.
6 months
TIMP-1
기간: 6 months
Number of TIMP-1 positive cells counted at x400 magnification (single field = 0.237 mm²) across three hot-spot fields, averaged per specimen. Compared between the VCMX and CTG groups.
6 months
MMP-1
기간: 6 months
Number of MMP-1 positive cells counted at x400 magnification (single field = 0.237 mm²) across three hot-spot fields, averaged per specimen. Compared between the VCMX and CTG groups.
6 months
Collagen I
기간: 6 months
Immunohistochemical Collagen I staining, quantified as the percentage of positively stained area relative to the total microscopic field area. Compared between the VCMX and CTG groups.
6 months
Collagen III
기간: 6 months
Immunohistochemical Collagen III staining, quantified as the percentage of positively stained area relative to the total microscopic field area. Compared between the VCMX and CTG groups.
6 months

공동 작업자 및 조사자

여기에서 이 연구와 관련된 사람과 조직을 찾을 수 있습니다.

스폰서

Ankara University

연구 기록 날짜

이 날짜는 ClinicalTrials.gov에 대한 연구 기록 및 요약 결과 제출의 진행 상황을 추적합니다. 연구 기록 및 보고된 결과는 공개 웹사이트에 게시되기 전에 특정 품질 관리 기준을 충족하는지 확인하기 위해 국립 의학 도서관(NLM)에서 검토합니다.

연구 주요 날짜

연구 시작 (실제)

2025년 12월 18일

기본 완료 (추정된)

2026년 9월 1일

연구 완료 (추정된)

2026년 9월 1일

연구 등록 날짜

최초 제출

2026년 8월 14일

QC 기준을 충족하는 최초 제출

2026년 8월 21일

처음 게시됨 (실제)

2026년 8월 24일

연구 기록 업데이트

마지막 업데이트 게시됨 (실제)

2026년 8월 24일

QC 기준을 충족하는 마지막 업데이트 제출

2026년 8월 21일

마지막으로 확인됨

2026년 8월 1일

추가 정보

이 연구와 관련된 용어

기타 연구 ID 번호

  • İ03-222-25
  • TSA-2025-4380 (기타 보조금/기금 번호: Ankara University Scientific Research Projects Unit)

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