The Aim of the Study is to Decipher the Alterations in Signalling Pathways of Skeletal Muscle Stem Cells (Satellite Cells (SCs)) in Individuals With COPD, as Well as to Examine the Effect of Exercise Training on SCs. (SCOOP)
Study of Satellite Cells in COPD-associated sarcOPenia (Satellite Cells in COPD Sarcopenia) : Exploratory Cohort Study for Translational Research
The aim of the study is to decipher the alterations in signalling pathways of skeletal muscle stem cells (satellite cells (SCs)) in individuals with COPD, as well as to examine the effect of exercise training on SCs. To this end, the team will perform an exhaustive evaluation of lung and muscle function, including exercise capacity, as well as muscle biopsies, before and after a 2-months rehabilitation program. This study should provide new therapeutic avenues for COPD-associated sarcopenia.
研究概览
详细说明
Chronic obstructive pulmonary disease (COPD) is now to be considered as a multimorbid disease, originating from a combination of several risk factors (respiratory events in childhood, exposure to inhaled particles, physical inactivity, etc.), and simultaneously affecting several organs. This multi-organ impairment is likely to arise from common biological processes such as systemic inflammation or repair failure. Studying these processes in an organ that is easily accessible to biopsy, such as skeletal muscle, can help in the overall management of COPD. Skeletal muscle wasting (or sarcopenia: loss of muscle mass and force) is also a major comorbidity in COPD, both frequent and associated to increased mortality. Muscle stem cells (satellite cells) are responsible for repairing skeletal muscle tissue after injury, notably after micro-lesions induced by exercise. However, they have been scarcely studied in COPD. In the present project, the team proposes to study repair abnormalities in skeletal muscle tissue by comparing the signalling pathways activated in satellite cells of patients with COPD, before and after a respiratory rehabilitation programme (20 sessions over 8 to 12 weeks). The team will carry out an exhaustive respiratory and muscular assessment at inclusion and at the end of the 20 sessions, evaluating in particular respiratory function and exercise capacity (Pulmonary Function Tests, cardio-pulmonary exercise testing with VO2max measurement, 6-minute walking test, handgrip test) as well as a quadricipital muscle biopsy with the modified Bergström's technique. Muscle biopsies will be used to culture satellite cells and assess their regenerative capacity in vitro, as well as to analyse dysregulated signalling pathways at the transcriptomic level with single-cell RNAsequencing. The results should help to identify new therapeutic avenues for the comprehensive management of multimorbid COPD.
研究类型
注册 (估计的)
阶段
- 不适用
联系人和位置
学习联系方式
- 姓名：Pauline HENROT, MD
- 电话号码：+33(0)5 57 65 65 13
- 邮箱：pauline.henrot@chu-bordeaux.fr
学习地点
-
-
-
Créteil、法国、94000
- Hôpital Henri-Mondor
-
接触：
- Laurent BOYER, MD, PhD
- 电话号码：+33(0)1 49 81 26 81
- 邮箱：laurent.boyer@aphp.fr
-
Paris、法国、75013
- Hôpital Pitié-Salpêtrière
-
接触：
- Romy RATSIHORIMANANA, MD
- 电话号码：+33(0)1 42 16 77 99
- 邮箱：romy.ratsihorimanana@aphp.fr
-
Pessac、法国、33400
- CHU de Bordeaux
-
接触：
- Pauline HENROT, MD
- 电话号码：+33(0)5 57 65 65 13
- 邮箱：pauline.henrot@chu-bordeaux.fr
-
首席研究员：
- Pauline HENROT, MD
-
-
参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
描述
Inclusion Criteria:
- Adult male or female (age 18 years or older);
- Having a diagnosis of COPD according to the GOLD (Global Initiative for Lung Disease) 2024 criteria, i.e. a non-reversible bronchial obstruction in spirometry with a post-bronchodilator Tiffeneau coefficient < 70%;
- Less than or equal to 75 years of age;
- Must benefit from exercise retraining sessions or respiratory rehabilitation as part of the management of COPD;
- Person affiliated to or beneficiary of a social security scheme (excluding State Medical Aid (AME));
- Free, informed and written consent signed by the participant or by a third party (in the event of the participant's physical disability) and the investigator (no later than the day of inclusion and before any review required by the research).
Exclusion Criteria:
- Moderate or severe acute exacerbation of COPD, or acute intercurrent condition (e.g., infection) that has been ongoing or resolved for less than 4 weeks;
- Systemic corticosteroid therapy in progress or stopped for less than 4 weeks;
- Contraindication to respiratory rehabilitation: tight aortic stenosis, unstable heart failure, active thromboembolic disease, ventricular aneurysm, uncontrolled arrhythmias;
- Contraindication to muscle biopsy: acquired or innate haemostasis disorders, anticoagulant treatment or double anti-platelet aggregation; contraindication to lidocaine: known hypersensitivity, patients with recurrent porphyrias;
- Presence of a concomitant muscle pathology, innate (e.g., genetic myopathy) or acquired (e.g., myositis, progressive cancer);
- Pregnant or breastfeeding woman;
- Patient under guardianship or curatorship;
- Refusal to participate in the study.
Exclusion criteria:
- Hemostasis disorders.
学习计划
研究是如何设计的？
设计细节
- 主要用途：治疗
- 分配：不适用
- 介入模型：单组作业
- 屏蔽：无（打开标签）
武器和干预
参与者组/臂 |
干预/治疗 |
|---|---|
|
实验性的：Men or women (adults) with a diagnosis of COPD according to GOLD criteria
|
Patients will undergo an exhaustive respiratory and muscular check-up (including quadridipital muscle biopsies) at inclusion and at the end of the respiratory rehabilitation program (a minimum of 20 sessions at a rate of 2 to 3 sessions per week for 8 to 12 weeks). |
研究衡量的是什么？
主要结果指标
结果测量 |
措施说明 |
大体时间 |
|---|---|---|
|
Change in single-cell gene expression profile of muscle satellite cells after pulmonary rehabilitation
大体时间：Day 1 (baseline, Visit 1) and Week 10 (± 2 weeks; Visit 2)
|
Intra-individual comparison of messenger RNA (mRNA) expression in muscle satellite cells isolated from quadriceps muscle biopsies collected before and after the pulmonary rehabilitation programme. Satellite cells will be isolated and analysed using single-cell and single-nucleus RNA sequencing. The outcome is the change in the gene-expression profile, including differentially expressed genes and associated molecular signalling pathways, between baseline and the end of rehabilitation. |
Day 1 (baseline, Visit 1) and Week 10 (± 2 weeks; Visit 2)
|
合作者和调查者
调查人员
- 首席研究员：Pauline HENROT、University Hospital, Bordeaux
研究记录日期
研究主要日期
学习开始 (估计的)
初级完成 (估计的)
研究完成 (估计的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (实际的)
研究记录更新
最后更新发布 (实际的)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
关键字
其他相关的 MeSH 术语
其他研究编号
- CHUBX 2026/005
- 2026-A00609-42 (其他标识符：French National Agency for Safety of Medicines and Health Products)
计划个人参与者数据 (IPD)
计划共享个人参与者数据 (IPD)？
IPD 计划说明
The data is available from Dr HENROT . An email can be sent to her so that she can contact the data manager and owner, the Bordeaux University Hospital. The data can be made available after contractual agreement.
IPD 共享支持信息类型
- 研究方案
- 国际碳纤维联合会
- 企业社会责任
药物和器械信息、研究文件
研究美国 FDA 监管的药品
研究美国 FDA 监管的设备产品
此信息直接从 clinicaltrials.gov 网站检索，没有任何更改。如果您有任何更改、删除或更新研究详细信息的请求，请联系 register@clinicaltrials.gov. clinicaltrials.gov 上实施更改，我们的网站上也会自动更新.