A Study of the Subcutaneous Injection of a Viscous Antibody Solution With Recombinant Human Hyaluronidase

Phase I Dose-Ranging Randomized Double-Blinded Controlled Study of the Subcutaneous Injection of a Viscous Antibody Solution With Recombinant Human Hyaluronidase

This Phase I, randomized, double-blinded, within-subject controlled, two-way crossover study comparing the time to inject (flow rate), safety, and tolerability of a subcutaneously (SC) administered, viscous antibody solution of with and without human recombinant hyaluronidase (rHuPH20) in volunteer subjects.

The study hypothesizes that the time required to complete a 20-mL SC injection of a viscous antibody with rHuPH20 will be comparable or shorter than the time required for the injection without rHuPH20.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Drug: Hylenex

• Study Type: Interventional
• Enrollment (Anticipated): 15
• Phase: Phase 1

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 65 years (Adult, Older Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: All

Description

Inclusion Criteria:

1. Volunteers of either sex, age 18 to 65 years, inclusive.
2. Intact normal skin on both upper extremities without tattoos or potentially obscuring pigmentation or lesions.
3. Vital signs (BP, HR, temperature, respiratory rate) within normal range.
4. Metabolic panel (e.g., sodium, potassium, chloride, bicarbonate, BUN, creatinine, glucose, calcium, AST, ALT, alkaline phosphatase, total bilirubin, albumin, and total protein) within normal range within 7 days of injection.
5. A negative serum or urine pregnancy test (if female of child-bearing potential) within 7 days of injection.
6. Female subjects of child-bearing potential must be practicing effective birth control or abstinence currently and plan to continue to do so for the duration of the study.
7. Decision-making capacity and willingness and ability to comply with the requirements for full completion of the trial.
8. Willingness and ability to sign an informed consent document.

Exclusion Criteria:

1. Upper extremity edema.
2. Upper extremity pathology that could interfere with any protocol-specified outcome assessment (e.g., cellulitis, lymphatic disorder or prior surgery, preexisting pain syndrome, previous mastectomy and/or axillary lymph node dissection, etc.).
3. Contraindication to an antibody, such as known history of anaphylactic or severe systemic reactions.
4. Known predisposition to renal insufficiency or renal failure, including diabetes mellitus, volume depletion, sepsis, paraproteinemia, and subjects receiving known nephrotoxic drugs.
5. Known allergy to hyaluronidase or any other ingredient in the formulation of Hylenex.
6. Known allergy to bee or vespid venom.
7. Known coagulopathy.
8. Pregnancy or breast-feeding woman.
9. Known clinically significant cardiovascular, gastrointestinal, hepatic, neurological, psychiatric, endocrine, cancer, HIV infection, diabetes mellitus, intercurrent illness such as influenza, or other major systemic disease that would unduly risk the subject's safety or interfere with the interpretation of results.
10. Participation in a study of any investigational drug or device within 30 days of enrollment in this study.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Health Services Research
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

What is the study measuring?

Primary Outcome Measures

Outcome Measure
The time to inject 20 mL of a viscous antibody solution with and without rHuPH20.

Secondary Outcome Measures

Outcome Measure
Assess the time required for a subcutaneous injection with various doses of rHuPH20, safety, tolerability.

Collaborators and Investigators

• Sponsor: Halozyme Therapeutics
• Investigators: Principal Investigator: Anoshie Ratnayake, M.D., Principal Investigator

Study record dates

Study Major Dates

• Study Start: February 1, 2007
• Study Completion (Actual): May 1, 2007

Study Registration Dates

• First Submitted: February 14, 2007
• First Submitted That Met QC Criteria: February 14, 2007
• First Posted (Estimate): February 15, 2007

Study Record Updates

• Last Update Posted (Estimate): October 17, 2011
• Last Update Submitted That Met QC Criteria: October 13, 2011
• Last Verified: February 1, 2007

More Information

Terms related to this study

• Keywords: hyaluronidase; subcutaneous drug delivery; Volunteer Subjects
• Other Study ID Numbers: HZ2-07-01