Cryosurvival of Embryos From Dysmorphic Oocytes

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Study Overview

• Status: Completed
• Conditions: Cryopreservation; Blastocyst
• Intervention / Treatment: Procedure: Cryopreservation, thawing and blastocyst culture

• Study Type: Observational

Contacts and Locations

Study Locations

• Turkey
  • Istanbul, Turkey, 34365
    • VKV American Hospital

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 45 years (Adult)
• Accepts Healthy Volunteers: N/A
• Genders Eligible for Study: Female

Description

Inclusion Criteria:

• Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
• Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion Criteria:

• Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
• Embryos obtained from abnormally fertilized zygotes.

Study Plan

How is the study designed?

Number of groups / cohorts

12

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Normal; Embryos derived from morphologically normal MII oocytes	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Irregular Shapes; Embryos derived from irregularly shaped oocytes.	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Large PVS; Embryos derived from oocytes with large perivitelline space.	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark Zona; Embryos derived from oocytes with dark zona pellucida	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark cytoplasm; Embryos derived from oocytes with dark cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Vacuolar cytoplasm; Embryos derived from oocytes with vacuolated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Central granulation; Embryos derived from oocytes with centrally granulated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double extra; Embryos derived from oocytes with double extracytoplasmic abnormalities	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double combined; Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double cytoplasmic; Embryos derived from oocytes with any two cytoplasmic anomalies	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple extra; Embryos derived from oocytes with triple extracytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple combined; Embryos derived from oocytes with triple combined anomalies	Procedure: Cryopreservation, thawing and blastocyst culture; Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Collaborators and Investigators

• Sponsor: V.K.V. American Hospital, Istanbul
• Investigators: Study Director: Basak Balaban, B.Sc., Assisted Reproduction Unit of the VKV American Hospital of Istanbul

Publications and helpful links

General Publications

• Balaban B, Ata B, Isiklar A, Yakin K, Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod. 2008 Aug;23(8):1778-85. doi: 10.1093/humrep/den127. Epub 2008 May 12.

Study record dates

Study Registration Dates

• First Submitted: August 24, 2007
• First Submitted That Met QC Criteria: August 24, 2007
• First Posted (Estimate): August 27, 2007

Study Record Updates

• Last Update Posted (Estimate): August 27, 2007
• Last Update Submitted That Met QC Criteria: August 24, 2007
• Last Verified: August 1, 2007

More Information

Terms related to this study

• Other Study ID Numbers: AH-05/04