Oxidative Stress as an Acute Exercise-induced Mechanism of Stem and Progenitor Cell Mobilization (EISMO)
December 13, 2019 updated by: Swiss Federal Institute of Technology
Effect of Oxidative Stress on Acute Exercise-induced Mobilization and Apoptosis of Hematopoietic, Endothelial and Mesenchymal Stem and Progenitor Cells and Mature Endothelial Cells in Healthy Young Male Subjects
It is already known from literature that exercise mobilizes stem and progenitor cells into the peripheral blood. However, the exact mechanisms thereof remain to be fully elucidated.
The investigators hypothesize that exercise-induced oxidative stress could be at least one of the responsible mechanisms and therefore want to study the exercise-induced stem and progenitor cell mobilization in a group of healthy young men when they exercise with, compared to when they exercise without antioxidative supplementation.
The primary outcome is numbers of stem and progenitor cells in the peripheral blood after an acute bout of exercise. As a secondary outcome, numbers of apoptotic mature and immature cells in the blood will be analysed.
Study Overview
Status
Completed
Conditions
Intervention / Treatment
Study Type
Interventional
Enrollment (Actual)
18
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Locations
-
-
Zurich
-
Zürich, Zurich, Switzerland, 8057
- Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
18 years to 35 years (Adult)
Accepts Healthy Volunteers
Yes
Genders Eligible for Study
Male
Description
Inclusion Criteria:
- Non-smoking
- Healthy
- Normal Body-Mass-Index (BMI): 18.5-24.9 kg∙m-2
- Active (fulfill World Health Organization-guidelines for recommended levels of physical activity for adults aged 18 - 64 years)
- Willing to adhere to the study rules
Exclusion Criteria:
- Known or suspected non-compliance, drug or alcohol abuse
- Inability to follow the procedures of the study, e.g. due to language problems, psychological disorders etc. of the participant
- Previous enrolment into the current study
- Enrolment of the investigator, his/her family members, employees and other dependent persons
- Regular intake of medication
- Regular intake of antioxidant supplementations
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Basic Science
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
|---|---|
|
Experimental: Antioxidative Supplementation
Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation
|
Each participant conducts two identical cycling tests, first without and a week later with antioxidative supplementation
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
Changes in exercise-dependently mobilized stem and progenitor cell numbers in the peripheral blood with antioxidative supplementation
Time Frame: On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
|
Numbers of hematopoietic, endothelial and mesenchymal stem and progenitor cells and mature endothelial cells in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.
|
On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
Changes in numbers of exercise-induced apoptotic cells and oxidative stress markers in the peripheral blood with antioxidative supplementation
Time Frame: On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
|
Numbers of apoptotic mononuclear cells, apoptotic hematopoietic, endothelial and mesenchymal stem and progenitor cells and apoptotic mature endothelial cells, as well as in oxidative stress markers in the peripheral blood will be measured after an acute bout of exercise first without and then with prior antioxidative supplementation.
|
On visit 2 after the exercise test without supplementation and then again on visit 3 after the exercise test with prior supplementation (1 week)
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Publications and helpful links
The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
May 2, 2019
Primary Completion (Actual)
October 21, 2019
Study Completion (Actual)
October 31, 2019
Study Registration Dates
First Submitted
November 12, 2018
First Submitted That Met QC Criteria
November 16, 2018
First Posted (Actual)
November 20, 2018
Study Record Updates
Last Update Posted (Actual)
December 16, 2019
Last Update Submitted That Met QC Criteria
December 13, 2019
Last Verified
December 1, 2019
More Information
Terms related to this study
Other Study ID Numbers
- EISMO
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
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