Effects of Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy (LYMPHOARESIS)

Comparison of the Effects of 2 Therapeutic Plasmapheresis Techniques: Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy (CIDP). A Monocentric Prospective Study With a Single-Case Experimental Design

CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.

Study Overview

• Status: Completed
• Conditions: Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
• Intervention / Treatment: Biological: Blood sampling

• Study Type: Interventional
• Enrollment (Actual): 8
• Phase: Early Phase 1

Contacts and Locations

Study Locations

• France
  • Nîmes, France
    • Olivier MORANNE

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 14 years and older (Adult, Older Adult)
• Accepts Healthy Volunteers: No

Description

Inclusion Criteria:

• Patients with confirmed Chronic Inflammatory Demyelinating Polyneuropathy (CIDP) without pathogenic antibodies and stable under therapeutic plasmapheresis with one session every 2-3 weeks for at least two months.
• Patients who have given free written informed consent.
• Patients who have signed the consent form.
• Patients affiliated to or beneficiaries of a health insurance scheme.
• Adult patients (≥18 ans).
• Healthy subjects of the same age and sex

Exclusion Criteria:

• Patients Under the age of 18.
• Patients under immunosuppressive treatment for less than 3 months.
• Patients participating in Category 1 research involving human subjects.
• Patients in an exclusion period determined by another study.
• Patients under legal protection, guardianship or curatorship.
• Patients unable to express consent.
• Patients for whom it is impossible to give informed information.
• Pregnant, parturient or breastfeeding patients.
• Healthy subject with chronic pathology or drug treatment

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Group A; Patients alternating single plasma exchange followed by double filtration plasmapheresis etc.	Biological: Blood sampling; Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis
Experimental: Group B; Patients alternating double filtration plasmapheresis followed by single plasma exchange etc.	Biological: Blood sampling; Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cycle 1, Group A : TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Time 0
Cycle 1, Group A : TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7 -10
Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.	Day 0, Time 0
Cycle 1, Group A : TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 1, Group A : T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7 - 10
Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day0, Hour 0
Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 7 - 10
Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.	Day 7 - 10
Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 2, Group B: TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group B: TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.	Day 7-10
Cycle 2, Group B: T17 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group B: TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 2, Group B: T-reg cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 2, Group B: T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 3, Group A: TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 1, Hour 1
Cycle 3, Group A: TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 1, Hour 3
Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 3, Group A: TH17 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.	Day 1, Hour 1
Cycle 3, Group A: TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 1, Hour 3
Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 7 - 10
Cycle 3, Group A: T-reg cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 3, Group A: T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7-10
Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %.	Day 7 - 10
Cycle 4, Group B : TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group B : TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7 -10
Cycle 4, Group B : TH17 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group B : TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %.	Day 7 -10
Cycle 4, Group B : T-reg cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 4, Group B : T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7 - 10
Cycle 5, Group A : TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group A : TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.	Day 7 - 10
Cycle 5, Group A : TH17 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group A : TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 7 - 10
Cycle 5, Group A : T-reg cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group A : T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7 - 10
Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7-10
Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 0
Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0, Hour 3
Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7-10
Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.	Day 7-10
Cycle 6, Group B : TH1 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group B : TH1 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.	Day 7-10
Cycle 6, Group B : TH17 cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group B : TH17 cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.	Day 7-10
Cycle 6, Group B : T-reg cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.	Day 0 Hour 0
Cycle 6, Group B : T-reg cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 0 Hour 3
Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.	Day 7-10

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 0
Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7 - 10
Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage.	Day 7-10
Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 0
Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0 Hour 3
Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 0
Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, Hour 3
Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H0
Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : B lymphocytes AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 0, H3
Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10
Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.	Day 7-10

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Group A, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Group B, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Group A, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Group B, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Group A, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Group B, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Group A, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Group B, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Group A, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Group B, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Group A, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Group B, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution .	All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle.	Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Group A, Cycle 1 : Cost of single plasma exchange to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Group B, Cycle 1 : Cost of double filtration plasmapheresis to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours)
Group A, Cycle 2 : Cost of double filtration plasmapheresis to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Group B, Cycle 2 : Cost of single plasma exchange to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours)
Group A, Cycle 3 : Cost of single plasma exchange to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Group B, Cycle 3 : Cost of double filtration plasmapheresis to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours)
Group A, Cycle 4 : Cost of double filtration plasmapheresis to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Group B, Cycle 4 : Cost of single plasma exchange to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours)
Group A, Cycle 5 : Cost of single plasma exchange to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Group B, Cycle 5 : Cost of double filtration to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours)
Group A, Cycle 6 : Cost of double filtration to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Group B, Cycle 6 : Cost of double filtration to the health institution.	The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles.	Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours)
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.	After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.	Day 0, Hour 0
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.	After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.	Day 0, Hour 3
Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping.	After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed.	Day 7-10
Compare lymphocyte phenotype between patients with PIDC and control subjects.	Significant difference in Th1/Th2 ratio between PIDC patients and control subjects.	Day 0

Collaborators and Investigators

• Sponsor: Centre Hospitalier Universitaire de Nīmes

Publications and helpful links

General Publications

• Moranne O, Ion IM, Cezar R, Messikh Z, Prelipcean C, Chkair S, Thouvenot E, Tran TA, Corbeau P, Chevallier T. Protocol of comparison of the effects of single plasma exchange and double filtration plasmapheresis on peripheral lymphocyte phenotypes in patients with Chronic Inflammatory Demyelinating Polyradiculoneuropathy: a monocentric prospective study with single-case experimental design. BMC Neurol. 2022 Aug 5;22(1):293. doi: 10.1186/s12883-022-02816-w.

Study record dates

Study Major Dates

• Study Start (Actual): March 12, 2021
• Primary Completion (Actual): December 18, 2023
• Study Completion (Actual): December 18, 2023

Study Registration Dates

• First Submitted: January 26, 2021
• First Submitted That Met QC Criteria: February 3, 2021
• First Posted (Actual): February 8, 2021

Study Record Updates

• Last Update Posted (Actual): December 10, 2025
• Last Update Submitted That Met QC Criteria: December 3, 2025
• Last Verified: December 1, 2025

More Information

Terms related to this study

• Keywords: Plasma exchange; Double filtration plasmapheresis
• Additional Relevant MeSH Terms: Nervous System Diseases; Pathologic Processes; Neuromuscular Diseases; Chronic Disease; Disease Attributes; Autoimmune Diseases; Immune System Diseases; Peripheral Nervous System Diseases; Autoimmune Diseases of the Nervous System; Demyelinating Diseases; Polyneuropathies; Polyradiculoneuropathy; Pathological Conditions, Signs and Symptoms; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating; Investigative Techniques; Specimen Handling; Clinical Laboratory Techniques; Diagnostic Techniques and Procedures; Diagnosis; Punctures; Surgical Procedures, Operative; Blood Specimen Collection
• Other Study ID Numbers: NIMAO/2020-01/OM001; 2020-A02879-30 (Other Identifier: ANSM)

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No