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Acute Blood Flow Restriction Walking on Immune Cell Phenotype and Function

3 novembre 2026 mis à jour par: Jonathan Little, University of British Columbia

Impact of Acute Blood Flow Restriction Walking on Immune Cell Phenotype and Function in Adults withT2D

To conduct a randomized control trial to determine the impact of acute blood flow restriction treadmill walking on immune cell counts, function, and phenotype in adults living with type 2 diabetes.

Aperçu de l'étude

Statut

Recrutement

Les conditions

Intervention / Traitement

Description détaillée

Participants will be randomized to a regular treadmill walking condition or treadmill walking with blood flow restriction (BFR) condition. In both groups participants will complete 32 minutes of treadmill walking at a speed and grade that elicit 40-50% heart rate reserve. In the BFR group the cuffs will be inflated based on each participants individualized limb occlusion pressure (LOP). The cuffs will remain inflated for the entire session but will deflate for 1 minute every 10 minutes based on safety recommendations. The percentage of LOP will 60%. Blood samples will be obtained pre-exercise (PRE), during the final 2 minutes of the final occlusion cycle (OCC), and 3 minutes into the recovery period post-exercise (POST) to measure outcomes.

Type d'étude

Interventionnel

Inscription (Estimé)

20

Phase

  • N'est pas applicable

Contacts et emplacements

Cette section fournit les coordonnées de ceux qui mènent l'étude et des informations sur le lieu où cette étude est menée.

Coordonnées de l'étude

Lieux d'étude

  • Canada
    • British Columbia
      • Kelowna, British Columbia, Canada, V1V 1V7
        • Recrutement
        • University of British Columbia Okanagan
        • Contact:

Critères de participation

Les chercheurs recherchent des personnes qui correspondent à une certaine description, appelée critères d'éligibilité. Certains exemples de ces critères sont l'état de santé général d'une personne ou des traitements antérieurs.

Critère d'éligibilité

Âges éligibles pour étudier

19 ans à 64 ans (Adulte)

Accepte les volontaires sains

Non

Sexes éligibles pour l'étude

Tout

La description

Inclusion Criteria:

  • Aged 19-64 years
  • Diagnosed with type 2 diabetes with current HbA1c 5.7-9.0%
  • Completing <150 minutes of moderate-vigorous physical activity per week.

Exclusion Criteria:

  • Chronic musculoskeletal condition or recent (within 2 years) cardiovascular event preventing participation in exercise.
  • Unstable type 2 diabetes medication of the last 3 months
  • Absolute contraindications to BFR (i.e., peripheral vascular disease)
  • A self-reported diagnosis of low iron concentrations, anemia, or being treated for these conditions
  • A diagnosis of any red blood cell-altering condition (i.e., sickle cell anemia, poikilocytosis)
  • Currently living with any cardiovascular disease, which would impact the ability to participate in exercise safely
  • Currently prescribed any medication which would impact the ability to use a heart rate monitor to accurately track intensity

Plan d'étude

Cette section fournit des détails sur le plan d'étude, y compris la façon dont l'étude est conçue et ce que l'étude mesure.

Comment l'étude est-elle conçue ?

Détails de conception

  • Objectif principal: Science basique
  • Répartition: Randomisé
  • Modèle interventionnel: Affectation parallèle
  • Masquage: Aucun (étiquette ouverte)

Armes et Interventions

Groupe de participants / Bras
Intervention / Traitement
Expérimental: Treadmill walking with blood flow restriction

Treadmill walking with blood flow restriction.
Autre: Exercise with BFR

Participants will complete 32 minutes of treadmill walking at a speed and grade that elicit 40-50% heart rate reserve. The cuffs will be inflated based on each participants individualized limb occlusion pressure (LOP). The cuffs will remain inflated for the entire session but will deflate for 1 minute every 10 minutes based on safety recommendations. The percentage of LOP will 60%.
Comparateur actif: Treadmill Walking

Treadmill walking.
Autre: Exercise

Participants will complete 32 minutes of treadmill walking at a speed and grade that elicit 40-50% heart rate reserve.

Que mesure l'étude ?

Principaux critères de jugement

Mesure des résultats
Description de la mesure
Délai
Change in white blood cell count before, during, and after exercise.
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The white blood cell count will be measured using a hematology analyzer before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise

Mesures de résultats secondaires

Mesure des résultats
Description de la mesure
Délai
Change in monocyte count before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The monocyte count will be measured using a hematology analyzer before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in neutrophil count before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The neutrophil count will be measured using a hematology analyzer before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in eosinophil count before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The eosinophil count will be measured using a hematology analyzer before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in CD66b+ expression before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The CD66b+ expression will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in CD11b+ expression before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The CD11b+ expression will be measured using flow cytometer before, during, and after exercise
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in CD63+ expression before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The CD63+ expression will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in % of classical monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The % of classical monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in % of intermediate monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The % of intermediate monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in % of non-classical monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The % of non-classical monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in TLR-4 expression on classical monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The TLR-4 expression on classical monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in TLR-4 expression on intermediate monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The TLR-4 expression on intermediate monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in TLR-4 expression on non-classical monocytes before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The TLR-4 expression on non-classical monocytes will be measured using flow cytometry before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in plasma IL-6 before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The plasma IL-6 concentration will be measured using a multiplex assay before, during, and after exercise
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in plasma IL-10 before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The plasma IL-10 concentration will be measured using a multiplex assay before, during, and after exercise.
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in plasma TNF-α before, during, and after exercise
Délai: Pre-exercise, during 32 minute of exercise, immediately post-exercise

The plasma TNF-α concentration will be measured using a multiplex assay before, during, and after exercise
Pre-exercise, during 32 minute of exercise, immediately post-exercise
Change in 4-h LPS-induced TNF-α before and after exercise
Délai: Pre-exercise, immediately post-exercise

The LPS-induced TNF-α concentration will be measured from whole-blood culture supernatant using an ELISA before and after exercise.
Pre-exercise, immediately post-exercise
Change in 4-h LPS+IL10-induced TNF-α before and after exercise
Délai: Pre-exercise, immediately post-exercise

The LPS+IL10-induced TNF-α concentration will be measured from whole-blood culture supernatant using an ELISA before and after exercise.
Pre-exercise, immediately post-exercise

Collaborateurs et enquêteurs

C'est ici que vous trouverez les personnes et les organisations impliquées dans cette étude.

Parrainer

University of British Columbia

Dates d'enregistrement des études

Ces dates suivent la progression des dossiers d'étude et des soumissions de résultats sommaires à ClinicalTrials.gov. Les dossiers d'étude et les résultats rapportés sont examinés par la Bibliothèque nationale de médecine (NLM) pour s'assurer qu'ils répondent à des normes de contrôle de qualité spécifiques avant d'être publiés sur le site Web public.

Dates principales de l'étude

Début de l'étude (Réel)

11 février 2026

Achèvement primaire (Estimé)

31 décembre 2027

Achèvement de l'étude (Estimé)

31 décembre 2027

Dates d'inscription aux études

Première soumission

23 juin 2026

Première soumission répondant aux critères de contrôle qualité

3 novembre 2026

Première publication (Réel)

10 novembre 2026

Mises à jour des dossiers d'étude

Dernière mise à jour publiée (Réel)

10 novembre 2026

Dernière mise à jour soumise répondant aux critères de contrôle qualité

3 novembre 2026

Dernière vérification

1 novembre 2026

Plus d'information

Termes liés à cette étude

Termes MeSH pertinents supplémentaires

Autres numéros d'identification d'étude

  • H25-02870 acute

Plan pour les données individuelles des participants (IPD)

Prévoyez-vous de partager les données individuelles des participants (DPI) ?

NON

Informations sur les médicaments et les dispositifs, documents d'étude

Étudie un produit pharmaceutique réglementé par la FDA américaine

Non

Étudie un produit d'appareil réglementé par la FDA américaine

Non

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