Meal-Exercise Challenge and Physical Activity Reduction Impact on Immunity and Inflammation (MERIIT)
In a society where exercise is prescribed by physicians to increase physical condition, improve dietary habits and reduce cardiovascular risk, the impact of exercise, acute or chronic, and diet in inflammation and immunity is unknown. Specific populations can have different responses towards acute and chronic exercise. Moreover specific conditions, like the type of meal ingested, can interfere with these responses. The true effect of pre-competitive meal exercise in the immunity and inflammatory response is unknown. Acute physical inactivity periods, commonly happens during daily life, related to work or acute illness. New evidence points out that it induces immediate metabolic and endocrinological changes, these can might also be associated with changes in immune response.
Aims of this project are:
- to assess the effect of the precompetitive meal on the inflammatory, neuro-immune and metabolic response to exercise;
- to assess the effects of sedentary behaviour on inflammatory, neuro-immune and metabolic response;
- to assess differences in changes between healthy, asthmatic and obese subjects
調査の概要
詳細な説明
Two interventions will be performed for assessing the research questions and aims:
A) Randomized cross-over, with a wash out period of 7 days, single-blinded trial where two different pre-competitive meals will be followed by an exercise challenge; inflammatory neuro-immune and metabolic response will be assessed before and after each challenge.
Participants will be recruited from advertisement and four different groups will be compared: obese-asthmatic; asthmatic non-obese; obese without asthma and non-asthmatic and non-obese. All patients will be allocated to a meal challenge in which a high fat poor micronutrient meal followed by a Mediterranean meal or vice-versa; meal challenge order will be randomized and participants will be blinded to the intervention. The main outcome for this intervention will be airway inflammation and lung function. All outcomes will be assessed before and 3h after each meal.
A group of individuals will, in the three hours after the meal, perform an exercise challenge, which is aimed to induce acute immune changes.
B) Randomized controlled trial where participants, after a run-in period and eligibility evaluation, will be allocated to an intervention consisting in a reduction of physical activity by 50% or maintaining habitual physical activity for two weeks; inflammatory neuro-immune and metabolic response will be assessed before and after intervention.
研究の種類
入学 (実際)
段階
- 適用できない
連絡先と場所
研究場所
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Porto、ポルトガル、4200
- Faculty of Nutrition and Food Sciences of the University of Porto
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Porto、ポルトガル、4200
- Faculty of Sports of Porto University
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Porto
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Portugal、Porto、ポルトガル、4200
- Faculty of Medicine of Porto University
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参加基準
適格基準
就学可能な年齢
健康ボランティアの受け入れ
受講資格のある性別
説明
Inclusion Criteria:
- controlled mild to moderate asthma;
- Body mass index (BMI) ≥ 25 kg/m2; healthy participants (non asthmatic with a BMI 18,5 to 25 kg/m2) will be used for comparison;
Exclusion Criteria:
- respiratory disease other than asthma;
- severe asthma according to Global Initiative for Asthma (GINA) guidelines
- occurrence in previous 4 weeks of screening of upper/lower respiratory tract infection;
- smoking in the past 6 months and requiring mechanical ventilation for respiratory event within 6 months of screening.
- Medical conditions (hematologic, cardiovascular, renal, hepatic, neurologic or metabolic) or medication that may interfere with meal or physical activity intervention;
- Significant chronic infectious diseases (eg, HIV, hepatitis B or C);
- Abnormal electrocardiogram screening, cardiac arrhythmia, angina, congestive heart failure; renal or hepatic failure and systemic disease, mal-absorption disease history or intestinal inflammatory disease;
- Women breast-feeding, pregnant or intending pregnancy during study
研究計画
研究はどのように設計されていますか？
デザインの詳細
- 主な目的：処理
- 割り当て：ランダム化
- 介入モデル：クロスオーバー割り当て
- マスキング：独身
武器と介入
参加者グループ / アーム |
介入・治療 |
|---|---|
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実験的：Meal Exercise Challenge- Order 2
Two different meals,an high fat micronutrient poor meal and Mediterranean Meal will be followed by an exercise challenge and performed at different orders accordingly to the cross over design.
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The meal will be prepared by a team of nutritionist in order to comply with the demand characteristics of an high fat poor micronutrient meal.
The meal will be prepared by a team of nutritionist in order to comply with the demand characteristics of an Mediterranean meal
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実験的：Meal Exercise Challenge- Order 1
Two different meals,an high fat micronutrient poor meal and Mediterranean Meal will be followed by an exercise challenge and performed at different orders accordingly to the cross over design. Each arm corresponds to a different intervention order. |
The meal will be prepared by a team of nutritionist in order to comply with the demand characteristics of an high fat poor micronutrient meal.
The meal will be prepared by a team of nutritionist in order to comply with the demand characteristics of an Mediterranean meal
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この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Airway inflammation
時間枠：Up to 6 months
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Up to 6 months
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二次結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Autonomic nervous system response
時間枠：Up to 6 months
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Rest heart rate; pupillary response, before and after challenge Neuropeptides measurement
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Up to 6 months
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Oxidative stress biomarkers
時間枠：Up to 6 months
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Assessment of plasma F2-isoprostanes, lipid hydroperoxides, protein carbonyls, hydrogen peroxide and lipid soluble antioxidants
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Up to 6 months
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Circulating blood leukocyte-total and differential leukocyte counts and lymphocyte subpopulations assessment.
時間枠：Up to 6 months
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Evaluated by flow cytometry: T cell (CD4+/CD8+), and their subsets; B cells (CD19+), Natural Killer (NK) cells (CD16+/56+), their subsets; monocytes subsets; oxidative burst activity
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Up to 6 months
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その他の成果指標
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Histamine skin test reactivity
時間枠：Up to 6 months
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Histamine skin test reactivity, assessed by skin prick test response
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Up to 6 months
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協力者と研究者
スポンサー
捜査官
- 主任研究者：Diana Silva, MD、Faculty of Medicine of Porto University
- 主任研究者：Rita Moreira、Faculty of Nutrition and Food Sciences of the University of Porto
出版物と役立つリンク
便利なリンク
研究記録日
主要日程の研究
研究開始
一次修了 (実際)
研究の完了 (実際)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (見積もり)
学習記録の更新
投稿された最後の更新 (見積もり)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
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