How Different Breathing Methods Affect Brain Activity in Young Adults With Chronic Ankle Instability (BRAIN-CAI)
Comparative Analysis of Breathing Intervention Types on Neural Activation in Young Adults With Chronic Ankle Instability: An fNIRS-EEG Randomized Controlled Trial
Chronic ankle instability is a common condition that can affect balance, movement control, and daily activities in young adults. Breathing exercises may influence brain function and attention, but their immediate effects on brain activity in individuals with chronic ankle instability are not well understood.
This randomized controlled trial aims to investigate the immediate effects of different breathing interventions on brain activity in young adults with chronic ankle instability. Participants will be randomly assigned to different breathing intervention groups. Brain activity will be assessed before and after the intervention using functional near-infrared spectroscopy (fNIRS) and electroencephalography (EEG). The findings may improve understanding of the relationship between breathing exercises and brain function and support the development of rehabilitation strategies for individuals with chronic ankle instability.
Studieöversikt
Status
Betingelser
Detaljerad beskrivning
Background and Rationale Chronic ankle instability (CAI) is a common sequela of lateral ankle sprain, characterized by recurrent giving way, perceived instability, and impaired dynamic balance. Growing evidence suggests that CAI involves not only peripheral deficits but also altered central nervous system processing, including changes in prefrontal cortical activity. Breathing-based interventions may influence postural control as well as cortical and autonomic regulation. However, it remains unclear how different types of breathing interventions compare in their immediate effects on brain activity in individuals with CAI. This study compared three breathing interventions: diaphragmatic breathing training, VR-based breathing meditation, and neurofeedback-based brain relaxation training.
Study Design This was a parallel-group, assessor-blinded randomized controlled trial. Thirty-three young adults with CAI were randomly allocated to one of three groups (n = 11 per group): the Diaphragmatic Breathing Group (DBG), the VR-based Breathing Meditation Group (VMG), or the Neurofeedback Brain Relaxation Training Group (NRG). Outcome assessors were blinded to group allocation. Each participant received a single intervention session according to group assignment, and outcomes were assessed before and immediately after the intervention. The study was designed and reported in accordance with the CONSORT guidelines.
Interventions The DBG performed diaphragmatic breathing training. The VMG performed breathing meditation delivered through a virtual reality environment. The NRG performed brain relaxation training based on EEG neurofeedback. The three groups differed in the type of breathing intervention while all received a single session. Detailed intervention procedures are described in the Arms and Interventions section.
Outcome Assessment The primary outcomes were cortical activity and EEG-based self-regulation indices. Cortical activity was measured by functional near-infrared spectroscopy (fNIRS) as oxygenated hemoglobin (HbO2) concentration in prefrontal regions of interest, including the dorsolateral prefrontal cortex, frontopolar cortex, orbitofrontal cortex, and medial prefrontal cortex. Neural self-regulation was evaluated using indices derived from EEG neurofeedback. The secondary outcomes were diaphragm contractility, measured as diaphragm thickening fraction using ultrasound; dynamic balance, measured by the Star Excursion Balance Test (SEBT); and ankle instability. Detailed outcome definitions and time frames are described in the Outcome Measures section.
Statistical Analysis Between-group differences were analyzed using analysis of variance (ANOVA), and within-group changes from pre- to post-intervention were analyzed using paired t-tests. Associations among fNIRS, EEG, and balance variables were examined using Pearson correlation analysis. Effect sizes were reported as Cohen's d. Bonferroni correction was applied where multiple comparisons were made. The significance level was set at 0.05.
Significance The findings of this study are expected to clarify how different types of breathing interventions immediately modulate prefrontal cortical activity and neural self-regulation in individuals with CAI, and to provide a basis for neuroscience-informed rehabilitation strategies for CAI.
Studietyp
Inskrivning (Beräknad)
Fas
- Inte tillämpbar
Kontakter och platser
Studieorter
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Gwangju, Sydkorea, 62287
- Rekrytering
- Gwangju Health University
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Kontakt:
- Hyeongju Yoon Yoon
- Telefonnummer: +82-10-5920-1504
- E-post: hyungju504506@naver.com
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Kontakt:
- Seungwoo Shin Shin, Ph.D.
- Telefonnummer: =82-10-9820-2382
- E-post: ptssw94@gmail.com
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Deltagandekriterier
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Åldrar som är berättigade till studier
Tar emot friska volontärer
Kön som är behöriga för studier
Beskrivning
Inclusion Criteria:
- Young adults
- CAIT-K score of ≤24
- A history of at least one ankle sprain occurring at least 12 months prior to study enrollment
- At least two episodes of ankle "giving way" within the 6months precending study enrollment
Exclusion Criteria:
- A sprain of the involved ankle within 3 months prior to study enrollment
- A History of lower-extremity surgery or fracture
- A history of neurological disorder
- The presence of cardiopulmonary disorder
- Self-reported symptoms of cybersickness
- Left-handedness
Studieplan
Hur är studien utformad?
Designdetaljer
- Primärt syfte: Grundläggande vetenskap
- Tilldelning: Randomiserad
- Interventionsmodell: Parallellt uppdrag
- Maskning: Enda
Vapen och interventioner
Deltagargrupp / Arm |
Intervention / Behandling |
|---|---|
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Aktiv komparator: Diaphragmatic brathing Training group
Participants in this group received diaphragmatic breathing training in a single session. Outcomes, including cortical activity, EEG indices, diaphragm contractility, dynamic balance, and ankle instability, were assessed before and immediately after the session. |
Participants assigned to this intervention will perform diaphragmatic breathing training in a single session. Participants will be guided to breathe using the diaphragm, and the training will be delivered under the supervision of a trained researcher. |
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Experimentell: VR-based breathing meditation group
Participants in this group received breathing meditation delivered through a virtual reality environment in a single session. Outcomes, including cortical activity, EEG indices, diaphragm contractility, dynamic balance, and ankle instability, were assessed before and immediately after the session. |
Participants assigned to this intervention will perform breathing meditation delivered through a virtual reality environment in a single session. Participants will wear a VR headset and follow the breathing meditation content presented in the virtual environment. |
|
Experimentell: Neurofeedback relaxation training group
Participants in this group received brain relaxation training based on EEG neurofeedback in a single session. Outcomes, including cortical activity, EEG indices, diaphragm contractility, dynamic balance, and ankle instability, were assessed before and immediately after the session. |
Participants assigned to this intervention will perform brain relaxation training based on EEG neurofeedback in a single session. Participants will receive real-time feedback on their EEG activity and will be trained to achieve a relaxed brain state. |
Vad mäter studien?
Primära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
|---|---|---|
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Change in dorsolateral prefrontal cortex oxygenated hemoglobin (HbO2) concentration measured by fNIRS
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Oxygenated hemoglobin (HbO2) concentration was measured by functional near-infrared spectroscopy (fNIRS) in the dorsolateral prefrontal cortex. Change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
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Change in frontopolar cortex oxygenated hemoglobin (HbO2) concentration measured by fNIRS
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Oxygenated hemoglobin (HbO2) concentration was measured by functional near-infrared spectroscopy (fNIRS) in the frontopolar cortex. Change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
|
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Change in orbitofrontal cortex oxygenated hemoglobin (HbO2) concentration measured by fNIRS
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Oxygenated hemoglobin (HbO2) concentration was measured by functional near-infrared spectroscopy (fNIRS) in the orbitofrontal cortex. Change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
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Change in medial prefrontal cortex oxygenated hemoglobin (HbO2) concentration measured by fNIRS
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Oxygenated hemoglobin (HbO2) concentration was measured by functional near-infrared spectroscopy (fNIRS) in the medial prefrontal cortex. Change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
|
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Change in EEG delta band power
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Delta band (0.5-4 Hz) power was measured by electroencephalography (EEG) at [electrode site(s)]. Change was calculated as the difference between pre- and post-intervention values. Unit: [µV²]. |
Baseline (before the intervention) and immediately after the single intervention session
|
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Change in EEG theta band power
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Theta band (4-8 Hz) power was measured by electroencephalography (EEG) at [electrode site(s)]. Change was calculated as the difference between pre- and post-intervention values. Unit: [µV²]. |
Baseline (before the intervention) and immediately after the single intervention session
|
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Change in EEG alpha band power
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
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Alpha band (8-12 Hz) power was measured by electroencephalography (EEG) at [electrode site(s)]. Change was calculated as the difference between pre- and post-intervention values. Unit: [µV²]. |
Baseline (before the intervention) and immediately after the single intervention session
|
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Change in EEG sensorimotor rhythm (SMR) band power
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Sensorimotor rhythm (SMR) band (12-15 Hz) power was measured by electroencephalography (EEG) at [electrode site(s)]. Change was calculated as the difference between pre- and post-intervention values. Unit: [µV²]. |
Baseline (before the intervention) and immediately after the single intervention session
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Change in EEG beta band power
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
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Beta band (15-30 Hz) power was measured by electroencephalography (EEG) at [electrode site(s)]. Change was calculated as the difference between pre- and post-intervention values. Unit: [µV²]. |
Baseline (before the intervention) and immediately after the single intervention session
|
Sekundära resultatmått
Resultatmått |
Åtgärdsbeskrivning |
Tidsram |
|---|---|---|
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Change in diaphragm thickening fraction measured by ultrasound
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Diaphragm thickness was measured by ultrasound at end-inspiration and end-expiration, and the diaphragm thickening fraction was calculated as (end-inspiration thickness - end-expiration thickness) / end-expiration thickness × 100 (%). Change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
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Change in dynamic balance measured by the Star Excursion Balance Test (SEBT)
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
|
Dynamic balance was assessed by the Star Excursion Balance Test (SEBT), in which participants reached as far as possible with the free limb while standing on the affected limb. Reach distance was measured in each direction, and change was calculated as the difference between pre- and post-intervention values. |
Baseline (before the intervention) and immediately after the single intervention session
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Change in ankle instability measured by CAIT-K
Tidsram: Baseline (before the intervention) and immediately after the single intervention session
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Ankle instability was assessed using [instrument name]. Change was calculated as the difference between pre- and post-intervention scores. |
Baseline (before the intervention) and immediately after the single intervention session
|
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- P01-202610-01-075
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De-identified individual participant data underlying the results reported in the publication will be shared with qualified researchers upon reasonable request to the corresponding author. Data will be available after publication of the main results.
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Data will be available beginning 6 months after publication of the main results
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Qualified researchers may request access to de-identified individual participant data by contacting the corresponding author. Requests will be reviewed by the research team, and data will be shared for academic research purposes.
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