Generation of Haploid Stem Cells From Human Germ Cells

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.

Study Overview

• Status: Unknown
• Conditions: Induced Pluripotent Stem Cells
• Intervention / Treatment: Other: generation of stem cells

Detailed Description

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.

• Study Type: Observational
• Enrollment (Anticipated): 10

Contacts and Locations

Study Locations

• Israel
  • Haifa, Israel, 30196
    • Not yet recruiting
    • Rambam Medical Center
    • Contact: Itay Maza, MD; Phone Number: 050-206-2609; Email: i_maza@rambam.health.gov.il
    • Principal Investigator: Itay Maza, MD
  • Rehovot, Israel, 76100
    • Recruiting
    • Weizmann Institute of Science
    • Contact: Itay Maza, MD; Phone Number: +972-8-934-2230; Email: itaym@weizmann.ac.il

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 50 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

• Sampling Method: Non-Probability Sample

Study Population

Male volunteers

Description

Inclusion Criteria:

1. males over the age of 18 and under 50
2. no known chronic illness
3. not taking any chronic drugs

Exclusion Criteria:

1. females
2. males under 18 or over 50
3. any known chronic illness
4. taking chronic medications

Study Plan

How is the study designed?

Number of groups / cohorts

1

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
Sperm sample from healthy volunteers	Other: generation of stem cells; Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded.; Other Names: generation of stem cells from somatic haploid germ cells

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Patients donated samples	No samples collected to date	June 2011-November 2013

Secondary Outcome Measures

Outcome Measure	Time Frame
No secomdary outcome	June 2011 - November 2013

Collaborators and Investigators

• Sponsor: Rambam Health Care Campus
• Investigators: Principal Investigator: Itay Maza, M.D, Rambam Health Care Campus

Study record dates

Study Major Dates

• Study Start: December 1, 2011
• Primary Completion (Anticipated): December 1, 2014
• Study Completion (Anticipated): August 1, 2015

Study Registration Dates

• First Submitted: October 16, 2011
• First Submitted That Met QC Criteria: October 18, 2011
• First Posted (Estimate): October 19, 2011

Study Record Updates

• Last Update Posted (Estimate): December 11, 2013
• Last Update Submitted That Met QC Criteria: December 10, 2013
• Last Verified: December 1, 2013

More Information

Terms related to this study

• Other Study ID Numbers: rmb-maza0001-ctil