Effects of Phototherapy by Low-level Light-emitting Diode Therapy (LEDT) on Clinical, Biochemical, Biomechanical, Histologic and Genetic Responses of Muscle Performance of Young Males Submitted to Physical Strength Training

Muscle performance is largely influenced by modulations in gene expressions of muscle tissue. In this context, light-emitting diode therapy (LEDT) has been used to improve muscle performance in experimental models and human researches. Thereby, the investigators examined modulations in gene expression of muscle tissue as well as biochemical, biomechanic and histologic adaptations influenced by exercise associated to LEDT.

Study Overview

• Status: Completed
• Conditions: Healthy
• Intervention / Treatment: Device: Effective light-emitting diode therapy; Device: Placebo light-emitting diode therapy

Detailed Description

This study assessed muscle performance in isokinetic dynamometry and led press device, gene expression by RT-PCR, lactate, creatine kinase and morphology of muscle tissue by immunohistochemical analysis

• Study Type: Interventional
• Enrollment (Actual): 32
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Brazil
  • São Paulo
    • São Carlos, São Paulo, Brazil, 13565-905
      • Federal University of São Carlos

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 28 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• healthy males aged between 18 and 28 years with a body mass index (BMI) equal to or less than 26 kg/m2,
• moderated pattern of physical activity

Exclusion Criteria:

• previous injury to the femoral quadriceps or hamstring muscles (within 6 months prior to study),
• osseous or articular disorder in the lower limbs,
• cardiovascular system disorders,
• systemic disease, and
• taking prescription medicines or using dietary supplements such as muscle mass builders.

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Double

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Active Comparator: Effective light-emitting diode therapy and training; Effects of effective light-emitting diode therapy therapy on muscle performance of young males submitted to physical strength training	Device: Effective light-emitting diode therapy; Other Names: LED; LED therapy; Light-emitting diode therapy
Placebo Comparator: Placebo light-emitting diode therapy and training; Effects of placebo light-emitting diode therapy on muscle performance of young males submitted to physical strength training	Device: Placebo light-emitting diode therapy; Other Names: LED; LED therapy; Light-emitting diode therapy

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Modulations on muscle gene expression by RT-PCR	12 weeks

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Muscle performance in leg press device		12 weeks
Muscle performance in leg extension device		12 weeks
Muscle performance in isokinetic dynamometer		12 weeks
Gene expression	Gene expression of muscle tissue by RT-PCR	12 weeks

Other Outcome Measures

Outcome Measure	Measure Description	Time Frame
Immunohistochemical analysis	Immunohistochemical analysis to quantify morphology and cells of muscle tissue as satellite cells.	12 weeks

Collaborators and Investigators

• Sponsor: Universidade Federal de Sao Carlos
• Collaborators: Fundação de Amparo à Pesquisa do Estado de São Paulo; Centro de Pequisas de Ótica e Fotônica
• Investigators: Principal Investigator: Cleber Ferraresi, MSc, Universidade Federal de Sao Carlos; Study Director: Nivaldo A Parizotto, PhD, Universidade Federal de Sao Carlos; Study Director: Vanderlei S Bagnato, PhD, University of São Paulo

Publications and helpful links

General Publications

• Vieira WH, Ferraresi C, Perez SE, Baldissera V, Parizotto NA. Effects of low-level laser therapy (808 nm) on isokinetic muscle performance of young women submitted to endurance training: a randomized controlled clinical trial. Lasers Med Sci. 2012 Mar;27(2):497-504. doi: 10.1007/s10103-011-0984-0. Epub 2011 Aug 26.
• Ferraresi C, de Brito Oliveira T, de Oliveira Zafalon L, de Menezes Reiff RB, Baldissera V, de Andrade Perez SE, Matheucci Junior E, Parizotto NA. Effects of low level laser therapy (808 nm) on physical strength training in humans. Lasers Med Sci. 2011 May;26(3):349-58. doi: 10.1007/s10103-010-0855-0. Epub 2010 Nov 18.

Study record dates

Study Major Dates

• Study Start: May 1, 2012
• Primary Completion (Actual): July 1, 2012
• Study Completion (Actual): September 1, 2014

Study Registration Dates

• First Submitted: September 15, 2012
• First Submitted That Met QC Criteria: January 15, 2013
• First Posted (Estimate): January 18, 2013

Study Record Updates

• Last Update Posted (Estimate): September 23, 2014
• Last Update Submitted That Met QC Criteria: September 19, 2014
• Last Verified: September 1, 2014

More Information

Terms related to this study

• Keywords: Gene expression; Functional performance; Muscle performance; Light-emitting diode therapy
• Other Study ID Numbers: CAAE: 0326.0.135.000-11