100-Year Human Aging Study

100-Year Human Aging Study: Prospective Longitudinal Validation of Multi-System Health Measurements Against Mortality and Aging Outcomes

The 100-Year Human Aging Study is a prospective, pragmatic, observational trial enrolling participants across fixed and mobile clinical sites to undergo comprehensive multi-system health screening and longitudinal follow-up until death. Participants are followed to determine whether measurements taken at enrollment and repeated across the lifespan - individually and in combination - predict all-cause mortality, cause-specific mortality, incident serious disease, and functional disability. The study is designed to generate the surrogate endpoint validation data that longevity medicine currently lacks.

Study Overview

• Status: Recruiting
• Conditions: Cardiovascular Diseases; Neoplasms; Mortality; Musculoskeletal Diseases; Cognitive Dysfunction; Metabolic Syndrome; Dementia; Frailty; Aging; Activities of Daily Living; Disability Physical; Neuro-Degenerative Disease; Aging Well; All-Cause Mortality; Health-Related Quality of Life
• Intervention / Treatment: Diagnostic test: Comprehensive Multi-System Clinical Screening

Detailed Description

All currently existing longevity measures are surrogate endpoints that have not been prospectively validated against actual mortality and aging outcomes. The 100-Year Human Aging Study is a prospective, pragmatic, observational trial that addresses this gap by enrolling participants in comprehensive clinical screening and following them longitudinally until death to determine which measurements - alone and in combination - are predictive of mortality, serious disease, and functional disability.

The study prioritizes dynamic measurements: the physiological, cognitive, social, and environmental capacities that change with aging and are most likely to carry predictive signal for mortality and functional outcomes. These include cardiorespiratory fitness (cardiopulmonary exercise testing with ventilatory threshold analysis), strength (grip, explosive power, functional movement), mobility and balance, neurocognitive performance, sensory function (vision, hearing, smell, light touch), and metabolic function (oral glucose tolerance, continuous glucose monitoring), in addition to other testing. Structural and imaging assessments include body composition and bone mineral density by DEXA, echocardiography, resting and stress electrocardiography, spirometry, retinal fundus photography, and vascular ultrasound. Laboratory measures are drawn on-site and processed through a CLIA-certified reference laboratory. Complete medical, surgical, family, social, occupational, and environmental histories are obtained at each visit.

Participation ranges from single-service visits - including standalone DEXA, cardiopulmonary exercise testing, and physician consultation - to the full two-visit comprehensive screening battery. All participation pathways contribute clinical data to the longitudinal mortality and aging outcomes linkage framework regardless of service level. Participants are encouraged to return for repeat testing to build longitudinal health trajectories across the lifespan.

At enrollment and across longitudinal follow-up, the study platform generates individualized investigational constructs including biological age estimate, predicted death age, and predicted cause of death profile. These are explicitly investigational hypotheses, not validated clinical standards. Their predictive validity relative to actual mortality, aging outcomes, and functional disability is a central scientific question this study is designed to answer - both for individual measures and for composite multi-system models.

All data are archived in their highest-dimensional raw form to preserve the ability to apply future analytical methods retroactively. Participants are followed with periodic contact and offered repeat screening throughout the lifespan. Longitudinal outcomes ascertainment includes all-cause mortality, cause-specific mortality, incident serious health events, chronic disease diagnosis, functional independence, disability status, and health behavior change.

• Study Type: Observational
• Enrollment (Estimated): 10000

Contacts and Locations

• Study Contact: Name: William E Brandenburg, MD; Phone Number: 3035010016; Email: info@longevitymetrics.org

Study Locations

• United States
  • Colorado
    • Boulder, Colorado, United States, 80301
      • Recruiting
      • Longevity Metrics
      • Contact: William E Brandenburg, MD; Phone Number: 3035010016; Email: info@longevitymetrics.org

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult; Older Adult
• Accepts Healthy Volunteers: Yes

• Sampling Method: Non-Probability Sample

Study Population

Participants of all ages, health statuses, and demographic backgrounds presenting for comprehensive preventive health screening at fixed or mobile clinical sites. No exclusions based on health status, geographic location, language, or population group. Participants with limited decision-making capacity may be enrolled with consent from a legally authorized representative. The study actively seeks to enroll participants across the full human lifespan including older adults and centenarians.

Description

Inclusion Criteria:

• Age 18 years or older
• Willing and able to provide written informed consent, or enrollment with consent of a legally authorized representative
• Willing to participate in longitudinal follow-up

Exclusion Criteria:

• Pregnancy
• Age under 18 years

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
All-Cause Mortality	Vital status ascertained through longitudinal follow-up contact, mortality record linkage, and health data network linkage using probabilistic matching on name, date of birth, and address history.	From enrollment until death, assessed periodically, up to 100 years
Cause-Specific Mortality	Cause of death ascertained via death certificate and mortality record linkage.	From enrollment until death, assessed periodically, up to 100 years

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Cause-Specific Mortality Prediction Accuracy	Concordance between predicted cause of death profile generated at enrollment and actual cause of death ascertained at follow-up.	From enrollment until death, assessed periodically, up to 100 years
Incident Serious Adverse Health Events	New diagnosis of myocardial infarction, stroke, cancer, dementia, heart failure, atrial fibrillation, sepsis, venous thromboembolism, COPD, chronic hypoxia, or major fracture ascertained via periodic follow-up contact and health data network linkage.	Periodically from enrollment until death, up to 100 years
Incident Chronic Disease	New diagnosis of type 2 diabetes, hypertension, COPD, chronic kidney disease, metabolic syndrome, or osteoporosis ascertained via self-report and health data network linkage.	Periodically from enrollment until death, up to 100 years
Functional Independence and Disability Status	Activities of daily living, instrumental activities of daily living, and self-reported disability status ascertained via periodic health survey using validated open-source instruments. Higher composite scores indicate greater functional independence. Specific instruments will be selected prior to first follow-up contact and documented in the statistical analysis plan.	Periodically from enrollment until death, up to 100 years
Biological Age Estimate Prediction Accuracy	Concordance between investigational biological age estimate and actual mortality outcomes at longitudinal follow-up.	Periodically from enrollment until death, up to 100 years
Health Behavior Change: Composite Self-Report and Repeat Screening Index	Health behavior change assessed through two complementary measures aggregated into a single index: validated composite self-report survey administered at periodic follow-up, and change in pre-specified clinical measurements at repeat screening. Improvement in objective clinical measurements (cardiorespiratory fitness, body composition, metabolic biomarkers, and related parameters) serves as the primary behavioral activation signal. Self-report domains including health behaviors and preventive care utilization provide supporting context. Both are combined into a single participant-level health activation index. Higher scores indicate greater engagement with health-promoting behaviors and measurable clinical improvement.	Periodically from enrollment until death, up to 100 years
Multi-System Predictor Modeling: Concordance Statistic	Predictive model performance quantified by concordance statistic (C-statistic) assessing the accuracy of a unified multi-domain measurement framework in predicting all-cause mortality and incident serious disease. The framework integrates clinical, biological, behavioral, social, occupational, and environmental data into a single composite predictive model. Analyses are reported as a single C-statistic for the full composite model, with pre-specified secondary reporting of C-statistics for individual predictor domains and domain combinations to identify which inputs are independently predictive, redundant, or synergistic. All analyses converge on the same unit of measure and the same outcome endpoints.	Periodically from enrollment until death, up to 100 years

Collaborators and Investigators

• Sponsor: Longevity Metrics, Inc.
• Investigators: Principal Investigator: William E Brandenburg, MD, Longevity Metrics, Inc.

Publications and helpful links

Helpful Links

• study website

Study record dates

Study Major Dates

• Study Start (Actual): February 9, 2025
• Primary Completion (Estimated): December 31, 2099
• Study Completion (Estimated): December 31, 2099

Study Registration Dates

• First Submitted: April 11, 2026
• First Submitted That Met QC Criteria: April 30, 2026
• First Posted (Actual): May 4, 2026

Study Record Updates

• Last Update Posted (Actual): May 4, 2026
• Last Update Submitted That Met QC Criteria: April 30, 2026
• Last Verified: April 1, 2026

More Information

Terms related to this study

• Keywords: Body Composition; Longevity; Population Health; Preventive Medicine; Functional Decline; Healthspan; Cardiopulmonary Exercise Testing; Biological Aging; Preventive Screening; Human Performance; Life Expectancy; Mortality Prediction; Surrogate Endpoint Validation; Longitudinal Cohort; Biomarker Validation; Centenarian
• Additional Relevant MeSH Terms: Brain Diseases; Central Nervous System Diseases; Nervous System Diseases; Mental Disorders; Pathologic Processes; Metabolic Diseases; Neurocognitive Disorders; Glucose Metabolism Disorders; Cognition Disorders; Insulin Resistance; Hyperinsulinism; Pathological Conditions, Signs and Symptoms; Nutritional and Metabolic Diseases; Frailty; Neoplasms; Cognitive Dysfunction; Cardiovascular Diseases; Metabolic Syndrome; Musculoskeletal Diseases; Dementia
• Other Study ID Numbers: 100Year; IORG0012336 (Other Identifier: OHRP); IRB00014601 (Other Identifier: OHRP)

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No