β2M Post-HD Rebound Prediction
Predicting the Post-dialysis Rebound of β2-microglobulin
β2-Microglobulin is small 11.8 kDa protein presents on the surface of all nucleated cell in the human body, it forms part of the non-variable chain of Major Histocompatibility Complex class I. β2-Microglobulin production is constant and it is continuously released into the bloodstream. Its production increases during active infections, inflammation or blood cancers. The Kidneys are the main route of its elimination. It accumulates in patients with kidney disease and levels can rise significantly in patients with end-stage renal failure. Studies have shown that its accumulation in dialysis patients contributes to dialysis-related amyloidosis.
Haemodialysis is a significant contributor to removal of β2-microglobulin in those on dialysis. It removes β2-microglobulin from the blood rather than directly from tissues. Blood levels fall during dialysis. However, after haemodialysis, β2-microglobulin gradually moves from tissues into the bloodstream until equilibrium is reached (post-dialysis rebound). Our own published data and unpublished data suggest this rebound occurs by two hours post-dialysis approximately but is significant in magnitude. β2-Microglobulin is increasingly recognized as an important marker of middle-molecule solute clearance.
The investigators plan to recruit thirty haemodialysis patients. During two dialysis sessions blood samples will be taken to measure β2-microglobulin. During the first session, samples will be taken pre-dialysis and at intervals during the session. Post-dialysis samples will be taken at 1 and 2 hour time points. The investigators will perform a physical examination, fluid/nutrition assessment. Dialysis prescription and routine monthly blood and urine results will also be recorded, and patients will be asked to complete questionnaires about dialysis symptoms, fatigue and post-dialysis recovery time. At the start of the next dialysis, one final β2-microglobulin sample will be taken.
β2-microglobulin removal may be a useful marker of dialysis quality. The investigators aim to develop a predictive model to estimate the equilibrated β2-microglobulin to allow its dialysis clearance to be accurately assessed.
Přehled studie
Postavení
Podmínky
Intervence / Léčba
Typ studie
Zápis (Odhadovaný)
Kontakty a umístění
Studijní kontakt
- Jméno: Shaza Adam, Clinical research Fellow
- Telefonní číslo: 01438287884340 00447365073834
- E-mail: Shaza.adam@nhs.net
Studijní záloha kontaktů
- Jméno: Enric Vilar, Consultant Nephrologist and Se
- E-mail: enric.vilar@nhs.net
Studijní místa
-
-
-
Stevenage, Spojené království, SG1 4AB
- Nábor
- East and North Hertfordshire NHS Trust
-
Kontakt:
- Toral Odedra, Research Sponsorship Coordinat
- E-mail: grantapplications.enh-tr@nhs.net
-
-
Kritéria účasti
Kritéria způsobilosti
Věk způsobilý ke studiu
- Dospělý
- Starší dospělý
Přijímá zdravé dobrovolníky
Metoda odběru vzorků
Studijní populace
Popis
Inclusion Criteria:
- Age 18 years or above.
- Ability to give informed consent.
- End Stage Renal Disease established on maintenance haemodialysis for at least 3 months
Exclusion Criteria:
- Inability to give informed consent.
- Acute infection in last 2 weeks.
Studijní plán
Jak je studie koncipována?
Detaily designu
Kohorty a intervence
Skupina / kohorta |
Intervence / Léčba |
|---|---|
|
End Stage Renal Disease on haemodialysis
Adult, End Stage Renal Disease established on maintenance haemodialysis for at least 3 months
|
Blood samples for β2-microglobulin measurement will be collected by study investigator across two haemodialysis sessions. During the first study visit (HD1), blood samples will be obtained pre-dialysis (0%), at 20%, 40%, 60%, and 80% of the prescribed dialysis duration, at the end of dialysis (100%), and at 1 hour and 2 hours following completion of dialysis. During the second study visit (HD2), a single pre-dialysis β2-microglobulin sample will be collected at the start of the participant's next routine haemodialysis session, which is second and last study visit. All study samples will be used to measure β2-Microglobulin concentration in mg/L |
Co je měření studie?
Primární výstupní opatření
Měření výsledku |
Popis opatření |
Časové okno |
|---|---|---|
|
Post-dialysis β2-microglobulin blood concentration (mg/L) at 1 and 2 hours after completion of haemodialysis
Časové okno: The study is very short study (Over 2 days)
|
β2-microglobulin blood samples will be collected at time points including pre-dialysis (0%), at 20%, 40%, 60%, 80%, and 100% of the dialysis session, as well as at 1 hour and 2 hours post-dialysis. An additional pre-dialysis sample will be obtained before the next routine haemodialysis session (approximately 48 hours later). The primary outcome measure will be the β2-microglobulin concentration (mg/L) at 1 and 2 hours post-dialysis, which will serve as the dependent variable in regression-based and software-assisted modelling. These models will be used to develop an algorithm capable of accurately predicting the equilibrated post-dialysis β2-microglobulin concentration from measurements obtained during the dialysis session. |
The study is very short study (Over 2 days)
|
Sekundární výstupní opatření
Měření výsledku |
Popis opatření |
Časové okno |
|---|---|---|
|
We will record small panel of solute clearance markers such as blood urea measured in mmol/L and creatinine umol/L
Časové okno: The study is very short study (Over 2 days)
|
This is recorded from the standard of care blood tests assessing monthly dialysis adequacy.
Derived from routine monthly dialysis adequacy testing done at same time period of the study sample collection.
|
The study is very short study (Over 2 days)
|
|
We will assess Patient-Reported Outcome Measures (PROMs) related to dialysis quality and dialysis-associated symptom burden, using validated questionnaires
Časové okno: The study is very short study (Over 2 days)
|
The following validated Questionnaires will be used to assess Patient Reported Outcome Measure (PROM): SONG-HD Fatigue, Recovery time and IPOS Renal questionnaires . The questionnaire burden was discussed at the Patients and Public Involvement Group meeting within the trust and considered in study design. The questionnaires were considered relevant and the burden on patients was judged to be acceptable and not a major barrier to participation. To minimise the participants being overwhelmed with questionnaires, we will offer them flexibility of completing the questionnaires during and or after first study visit (either on dialysis or at home). They may also receive support from family, friend and or medical staff team to help filling these questionnaires as needed. It is anticipated that each questionnaire will take approximately 5 minutes to complete. We expect most patients will complete this during their dialysis treatment |
The study is very short study (Over 2 days)
|
Spolupracovníci a vyšetřovatelé
Vyšetřovatelé
- Vrchní vyšetřovatel: Shaza Adam, Lister Hospital, East and North Hertfordshire Teaching NHS Trust
Termíny studijních záznamů
Hlavní termíny studia
Začátek studia (Aktuální)
Primární dokončení (Odhadovaný)
Dokončení studie (Odhadovaný)
Termíny zápisu do studia
První předloženo
První předloženo, které splnilo kritéria kontroly kvality
První zveřejněno (Aktuální)
Aktualizace studijních záznamů
Poslední zveřejněná aktualizace (Aktuální)
Odeslaná poslední aktualizace, která splnila kritéria kontroly kvality
Naposledy ověřeno
Více informací
Termíny související s touto studií
Další relevantní podmínky MeSH
- Urogenitální onemocnění
- Patologické procesy
- Mužská urogenitální onemocnění
- Onemocnění ledvin
- Urologická onemocnění
- Ženské urogenitální onemocnění
- Ženské urogenitální onemocnění a těhotenské komplikace
- Chronické onemocnění
- Atributy nemoci
- Renální insuficience
- Renální insuficience, chronická
- Patologické stavy, příznaky a symptomy
- Selhání ledvin, chronické
Další identifikační čísla studie
- RD2025-60
Plán pro data jednotlivých účastníků (IPD)
Plánujete sdílet data jednotlivých účastníků (IPD)?
Informace o lécích a zařízeních, studijní dokumenty
Studuje lékový produkt regulovaný americkým FDA
Studuje produkt zařízení regulovaný americkým úřadem FDA
Tyto informace byly beze změn načteny přímo z webu clinicaltrials.gov. Máte-li jakékoli požadavky na změnu, odstranění nebo aktualizaci podrobností studie, kontaktujte prosím register@clinicaltrials.gov. Jakmile bude změna implementována na clinicaltrials.gov, bude automaticky aktualizována i na našem webu .
Klinické studie na Onemocnění ledvin, konečná fáze
-
Ionis Pharmaceuticals, Inc.BayerDokončenoEnd-stage Renal Disease (ESRD)Španělsko
-
National Taiwan University HospitalNeznámý
-
Ottawa Hospital Research InstituteHeart and Stroke Foundation of OntarioDokončenoEnd-stage Renal Disease (ESRD) | Nemoc ledvinKanada
-
Ionis Pharmaceuticals, Inc.BayerDokončenoEnd-stage Renal Disease (ESRD)Kanada
-
Rockwell Medical Technologies, Inc.DokončenoEnd-stage Renal Disease (ESRD)Spojené státy, Kanada
-
University of Kansas Medical CenterDokončenoEnd-stage Renal Disease (ESRD) | Chronické onemocnění ledvin (CKD)Spojené státy
-
The First People's Hospital of YunnanAktivní, ne náborEnd-stage Renal Disease (ESRD) | Údržbová hemodialýza (MHD)Čína
-
Universitas Muhammadiyah SurakartaDokončenoHemodialýza | Chronické onemocnění ledvin | End-stage Renal Disease (ESRD)Indonésie
-
Shanghai 10th People's HospitalZápis na pozvánkuNutriční stav | Renální substituční terapie | End-stage Renal Disease (ESRD) | Peritoneální dialýza (PD)Čína
-
Darya ChamaniDokončenoHemodialýza | End-stage Renal Disease (ESRD)Írán
Klinické studie na β2-microglobulin blood concentration in mg/L
-
City of Hope Medical CenterNational Cancer Institute (NCI)NáborAkutní myeloidní leukémie | Akutní lymfoblastická leukémie | Myelodysplastický syndromSpojené státy