the Effects of Different Dietary Phosphorus Intake on the Circadian Pattern of Serum Phosphate in Normal Subjects
Aperçu de l'étude
Statut
Les conditions
Intervention / Traitement
Description détaillée
Emerging evidence show that serum phosphate concentrations are not only associated with cardiovascular and all-cause mortality in chronic kidney disease (CKD) patients, but also associated with the mortality in community population (without history of CKD or cardiovascular disease). The rise of serum phosphate will lead to the elevation of serum plasma fibroblast growth factor 23 (FGF23) and parathyroid hormone (PTH) in healthy people, contributing to bone disorders and left ventricle thickening. Therefore, maintaining normal serum phosphate and reducing postprandial fluctuation of serum phosphate are essential for CKD patients as well as healthy people.
As for serum phosphate modulation by diet, most evidence was focused on CKD patients, less focused on healthy people. An American study in 1980s indicated that phosphorus intake was significantly related with the level of 24-h serum phosphate and the circadian pattern of serum phosphate. However, this study failed to explore the underlying mechanisms and the diet in America is different from that in China. Nowadays, normal diet in China is inclined to having more calories and protein, which warrants high diet phosphorus intake, may contributing to huge fluctuation of serum phosphate and thus elevating the incidence and mortality of cardiovascular disease. Therefore, what is the level and circadian pattern of serum phosphate concentrations in normal subjects under our native normal diet in China? Is restricted phosphate diet able to alter the level and circadian pattern of serum phosphate? These questions are what the investigator sare eager to answer.
Accumulating evidence suggest that gastrointestinal tract has a vital effect in phosphate modulation. Berndt etc. suggested that in rat, epidermal cells of duodenum and jejunum could sense the gastrointestinal tract phosphate load, rapidly activating the type IIa Na/Pi-cotransporter in the renal tubule cells, leading to the increase of urinary phosphate, without any changes of serum FGF23, PTH and phosphate concentrations. So the author proposed that there had a gastro intestine-kidney axis in phosphate modulation. However, whether this axis exists in human body remains unclear.
Therefore, the investigators plan to conduct a crossover clinical study to evaluate the level and circadian pattern of serum phosphate concentrations modulated by native normal diet and restricted phosphate diet and then to explore the underlying mechanisms.
Type d'étude
Inscription (Réel)
Phase
- N'est pas applicable
Contacts et emplacements
Lieux d'étude
-
-
Shanghai
-
Shanghai, Shanghai, Chine, 200040
- Huashan Hospital, Shanghai Medical College, Fudan University
-
-
Critères de participation
Critère d'éligibilité
Âges éligibles pour étudier
Accepte les volontaires sains
Sexes éligibles pour l'étude
La description
Inclusion Criteria:
- Men aged 18-45 years
- Healthy volunteers: no history of chronic diseases; no active symptoms or physical signs; negative results of electrocardiogram and chest X-ray; biochemical indicators of regular medical examination are within 95%-105% of normal range
- Willingness to sign the consent form approved by an Institutional Review Board and comply with the study protocol
- Body mass index ranged within 18.5-24 kg/m2
Exclusion Criteria:
- Current history of drug or alcohol abuse as assessed by the principal Investigator
- Subject has blood donations or blood loss more than 300ml within three months
- Allergic to more than two foods or drugs
- Subject has psycho-disability or body disability
- Subject has a condition that in the judgment of the Principal Investigator could potentially pose a health risk to the patient while involved in the study
- Subject has participated in the other clinical trials at the same time
Plan d'étude
Comment l'étude est-elle conçue ?
Détails de conception
- Répartition: Non randomisé
- Modèle interventionnel: Affectation croisée
- Masquage: Aucun (étiquette ouverte)
Armes et Interventions
Groupe de participants / Bras |
Intervention / Traitement |
|---|---|
|
Expérimental: Régime normal de phosphore
Régime contenant 1500 mg de phosphore par jour
|
Régime contenant 1500 mg de phosphore
|
|
Expérimental: Restricted Phosphorus Diet
Diet containing 500mg of phosphorus per day
|
Diet containing 500mg of phosphorus
|
Que mesure l'étude ?
Principaux critères de jugement
Mesure des résultats |
Délai |
|---|---|
|
Rythme circadien de la concentration sérique de phosphore
Délai: 24 heures
|
24 heures
|
Mesures de résultats secondaires
Mesure des résultats |
Délai |
|---|---|
|
Rythme circadien du facteur de croissance plasmatique des fibroblastes 23 (FGF23)
Délai: 24 heures
|
24 heures
|
|
Rythme circadien de l'hormone parathyroïdienne (PTH)
Délai: 24 heures
|
24 heures
|
|
Rythme circadien de la 1,25-dihydroxyvitamine D (1,25(OH)2D3)
Délai: 24 heures
|
24 heures
|
|
Circadian rhythm of matrix extracellular phosphoglycoprotein (MEPE)
Délai: 24 hour
|
24 hour
|
Collaborateurs et enquêteurs
Parrainer
Dates d'enregistrement des études
Dates principales de l'étude
Début de l'étude
Achèvement primaire (Réel)
Achèvement de l'étude (Réel)
Dates d'inscription aux études
Première soumission
Première soumission répondant aux critères de contrôle qualité
Première publication (Estimation)
Mises à jour des dossiers d'étude
Dernière mise à jour publiée (Estimation)
Dernière mise à jour soumise répondant aux critères de contrôle qualité
Dernière vérification
Plus d'information
Termes liés à cette étude
Mots clés
Autres numéros d'identification d'étude
- HuashanH
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