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Proof of Principle Study for an Efficacy Trial of Linaclotide for Cystic Fibrosis (MODEL)

28 avril 2026 mis à jour par: Robin Spiller, University of Nottingham

A Randomised, Placebo-controlled Crossover Study Defining the Mode of Action of Linaclotide in Healthy Volunteers Using MRI

Linaclotide is a medicine used to treat constipation and irritable bowel syndrome with constipation (IBS-C). It works by acting on the surface of the gut lining, where it increases the movement of salt and water into the bowel. This softens stools, makes them easier to pass, and can also reduce gut pain

One advantage of linaclotide is that, unlike some natural substances in the gut, it is stable and can act throughout the intestine. Studies in animals show that it has the strongest effect in the upper small intestine, but it may act in other parts of the bowel as well. In people, however, it is not yet clear whether linaclotide mainly works in the small intestine or in the large intestine (colon). Knowing this is important, because it could help the investigators understand whether linaclotide might also be useful in other conditions, such as cystic fibrosis, where the gut does not handle fluid properly.

Linaclotide is taken as a capsule, but less than 1% is absorbed into the bloodstream. Instead, it stays in the gut, where it is broken down into smaller active parts. This means both the small intestine and colon may be exposed to its effects.

Until now, it has been hard to study this because traditional methods only measure one part of the gut at a time. A team at the University of Nottingham has developed MRI scanning methods that can safely and non-invasively measure water content in the small intestine and colon.

The aim of this pilot study is to use MRI in healthy volunteers to see exactly where linaclotide acts. This knowledge will help optimise future studies in conditions such as cystic fibrosis.

Aperçu de l'étude

Statut

Recrutement

Les conditions

Intervention / Traitement

Description détaillée

Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the CF transmembrane conductance regulator (CFTR) gene. In CF, defective CFTR may lead to various clinical effects on the gastrointestinal (GI) system; indeed, many CF patients report significant GI symptoms, with the most frequent being attributable to the lower GI tract, including bloating, flatulence, abdominal pain, and borborygmi. Moreover, constipation is another prevalent GI symptom in CF patients. It is estimated that 10-57% of CF patients report constipation symptoms, with an even higher prevalence (approximately 73%) in adults over the age of 30. Symptoms such as constipation are hypothesised to occur due to decreased luminal fluid content resulting from a reduction in anion flow, which ultimately leads to increased amounts of viscous mucus and slowed transit of intestinal contents. Presently, these GI symptoms in CF patients are often treated with laxatives and enemas, and in some cases surgical treatment, which can be uncomfortable and invasive, suggesting that alternative treatments are required.

Linaclotide, a drug approved for safe use across Europe and in the UK, is a synthetic analogue of uroguanylin that activates guanylate cyclase-C (GC-C) receptors located on the luminal surface of intestinal epithelial cells. Activation of GC-C increases intracellular cyclic guanosine monophosphate (cGMP), which stimulates cGMP-dependent protein kinase II (PKGII) and protein kinase A (PKA). Collectively, these kinases activate CFTR, leading to chloride and water secretion into the intestinal lumen. In parallel, elevated cGMP inhibits the sodium-hydrogen exchanger 3 (NHE3), thereby reducing sodium and water absorption. Together, these actions promote fluid secretion and accelerate intestinal transit, leading to linaclotide being commonly prescribed for the treatment of chronic idiopathic constipation and irritable bowel syndrome with chronic constipation (IBS-C).

Meta-analyses confirm linaclotide's clinical benefit in chronic idiopathic constipation and IBS-C; however, its role in CF remains uncertain, though anecdotal reports and animal models suggest therapeutic potential. Linaclotide has less than 1% systemic bioavailability and is degraded in the upper small bowel by carboxypeptidases to an active metabolite, with a small proportion of the administered dose recovered in stool, providing exposure throughout the small and large intestine. However, the primary site of action in humans remains undefined.

Traditional perfusion methods provide only segmental information, limiting assessment of the whole intestine. By contrast, magnetic resonance imaging (MRI) enables non-invasive evaluation of gastrointestinal water content across multiple regions. The Nottingham group has validated MRI techniques for quantifying small bowel water content and assessing colonic chyme hydration via T1 mapping. These methods have been successfully applied to study the effects of various pharmacological agents on gastrointestinal function.

Aim: To define the site of action of linaclotide in healthy volunteers using MRI. Identifying the regional effects of linaclotide will help optimise its future evaluation in CF patients. Based on clinical observations in constipation, stool effects emerge within seven days; however, the investigators hypothesise that small bowel changes occur within 1-2 hours of dosing. Therefore, a one-day pre-dosing regimen is expected to elicit a measurable effect on both the small bowel and colon while minimising participant burden.

Subjects will take 290ug linaclotide /placebo on the day prior to study day (day -1) and on the study day

Type d'étude

Interventionnel

Inscription (Estimé)

26

Phase

  • Première phase 1

Contacts et emplacements

Cette section fournit les coordonnées de ceux qui mènent l'étude et des informations sur le lieu où cette étude est menée.

Coordonnées de l'étude

Lieux d'étude

Critères de participation

Les chercheurs recherchent des personnes qui correspondent à une certaine description, appelée critères d'éligibilité. Certains exemples de ces critères sont l'état de santé général d'une personne ou des traitements antérieurs.

Critère d'éligibilité

Âges éligibles pour étudier

  • Adulte
  • Adulte plus âgé

Accepte les volontaires sains

Oui

La description

Inclusion Criteria:

Participant is willing and able to give informed consent for participation in the study

Not currently taking any medications (except for selective serotonin reuptake inhibitors, low dose tricyclic antidepressants, antihistamines, and oral contraceptive pill).

Aged between 18-60 years.

Ability to conform to the study protocol, including overnight fasting, dietary and lifestyle restriction, administering linaclotide and placebo intervention, MRI scanning, consuming the rice pudding/blue dye meal, and rating stool frequency and appearance.

Exclusion Criteria:

Contraindication to MRI scanning (i.e. metallic implants, pacemakers, history of metallic foreign body in eye(s) and penetrating eye injury, unable to lie flat and relatively still for less than 5 minutes.)

Pregnancy, lactating, or planning pregnancy during the investigation declared by candidate.

History declared by the candidate of pre-existing gastrointestinal disorder that may affect bowel function.

Reported history of previous resection of the oesophagus, stomach, or intestine (excluding appendix).

Intestinal stoma.

Any medical condition that may potentially compromise participation in the study e.g., known food intolerance to rice pudding, known contraindication to the oral administration of linaclotide or placebo.

Has a body mass index (BMI) value less than 18.5 or greater than 35.

Will not agree to follow dietary and lifestyle restrictions required.

Unable to stop drugs known to alter GI motility including mebeverine, opiates, monoamine oxidase inhibitors, phenothiazines, benzodiazepines, calcium channel antagonists for the duration of the study.

Participants who are currently (or in the past 3 months) taking antibiotics or probiotics as these may impact GI function.

Participation in night shift work the week prior to the study day. Night work is defined as working between midnight and 6.00 AM.

Anyone who in the opinion of the investigator is unlikely to be able to comply with the protocol e.g., cognitive dysfunction, chaotic lifestyle related to substance abuse.

Having taken part in a research study in the last 3 months involving invasive procedures or an inconvenience allowance

-

Plan d'étude

Cette section fournit des détails sur le plan d'étude, y compris la façon dont l'étude est conçue et ce que l'étude mesure.

Comment l'étude est-elle conçue ?

Détails de conception

  • Objectif principal: Traitement
  • Répartition: Randomisé
  • Modèle interventionnel: Affectation croisée
  • Masquage: Tripler

Armes et Interventions

Groupe de participants / Bras
Intervention / Traitement
Expérimental: Linaclotide
290 mcg linaclotide across 2 days
Médicament: Linaclotide 290 micrograms
290 mcg, 2 days dosing, oral capsule form
Comparateur placebo: Placebo
Lactose placebo
Médicament: Lactose placebo pill
Placebo form, oral capsules identical to linaclotide

Que mesure l'étude ?

Principaux critères de jugement

Mesure des résultats
Description de la mesure
Délai
Small bowel water content
Délai: Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Area under curve (AUC) 0-360 minutes Water content in small bowel as assessed by MRI (mL)
Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose

Mesures de résultats secondaires

Mesure des résultats
Description de la mesure
Délai
Colon water content
Délai: Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Area under curve (AUC) 0-360 min Water content in the ascending colonic region as assessed by MRI (mL)
Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Colonic regional segmental volumes
Délai: Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Total volume of regional colonic segments (ascending, transverse, descending, sigmorectal) as assessed by MRI (mL) at each time point.
Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Changes in stool consistency
Délai: 2 days before intervention, 5 days post-intervention
Stool consistency rated using Bristol stool scale (type 1-7; 7 being watery stool).
2 days before intervention, 5 days post-intervention
Changes in whole gut transit time (WGTT)
Délai: 1 day post-intervention
Assessed by time to stool discolouration following administration of blue dye paste.
1 day post-intervention
Gastrointestinal symptom rating
Délai: 2 days pre-intervention and 5 days post-intervention

Assessing the severity of common gastrointestinal symptoms (flatulence / gas passage, diarrhoea / loose stool, bloating, abdominal pain) via Likert-type scale: 0 = not at all

  1. = mild (distinct but negligible)
  2. = moderate (annoying)
  3. = severe (disabling
2 days pre-intervention and 5 days post-intervention
Changes in stool frequency
Délai: 2 days before starting intervention and for 5 days post intervention
Frequency (per day) of bowel movements (number with exact time)
2 days before starting intervention and for 5 days post intervention
Small bowel motility
Délai: Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose
Maximum motility score (A.U) of the small bowel as assessed by MRI
Baseline, and 0, 60, 120, 180, 240, 300, 360 minutes post-dose

Collaborateurs et enquêteurs

C'est ici que vous trouverez les personnes et les organisations impliquées dans cette étude.

Parrainer

University of Nottingham

Publications et liens utiles

La personne responsable de la saisie des informations sur l'étude fournit volontairement ces publications. Il peut s'agir de tout ce qui concerne l'étude.

Publications générales

  • Wilkinson-Smith, V., Hoad, C., Atkinson, D., Marciani, L., Corsetti, M., Scott, S. M., Taylor, S., Gowland, P., & Spiller, R. (2021). O59 MRI methods to define colonic function in health and constipation. Gut, 70(Suppl 1), A32-A33. https://doi.org/10.1136/GUTJNL-2020-BSGCAMPUS.59
  • Stefano, M. A., Sandy, N. S., Zagoya, C., Duckstein, F., Ribeiro, A. F., Mainz, J. G., & Lomazi, E. A. (2022). Diagnosing constipation in patients with cystic fibrosis applying ESPGHAN criteria. Journal of Cystic Fibrosis, 21(3), 497-501. https://doi.org/10.1016/j.jcf.2021.08.021
  • Stefano, M. A., Poderoso, R. E., Mainz, J. G., Ribeiro, J. D., Ribeiro, A. F., & Lomazi, E. A. (2020). Prevalence of constipation in cystic fibrosis patients: a systematic review of observational studies. Jornal de Pediatria, 96(6), 686-692. https://doi.org/10.1016/j.jped.2020.03.004
  • Rubinstein, S., Moss, R., & Lewiston, N. (1986). Constipation and Meconium Ileus Equivalent in Patients With Cystic Fibrosis. Pediatrics, 78(3), 473-479. https://doi.org/10.1542/PEDS.78.3.473
  • McHugh, D. R., Cotton, C. U., Moss, F. J., Vitko, M., Valerio, D. M., Kelley, T. J., Hao, S., Jafri, A., Drumm, M. L., Boron, W. F., Stern, R. C., McBennett, K., & Hodges, C. A. (2018). Linaclotide improves gastrointestinal transit in cystic fibrosis mice by inhibiting sodium/hydrogen exchanger 3. American Journal of Physiology - Gastrointestinal and Liver Physiology, 315(5), G868. https://doi.org/10.1152/AJPGI.00261.2017
  • arciani, L., Wright, J., Foley, S., Hoad, C. L., Totman, J. J., Bush, D., Hartley, C., Armstrong, A., Manby, P., Blackshaw, E., Perkins, A. C., Gowland, P. A., & Spiller, R. C. (2010). Effects of a 5-HT(3) antagonist, ondansetron, on fasting and postprandial small bowel water content assessed by magnetic resonance imaging. Alimentary Pharmacology & Therapeutics, 32(5), 655-663. https://doi.org/10.1111/J.1365-2036.2010.04395.X
  • Marciani, L., Garsed, K. C., Hoad, C. L., Fields, A., Fordham, I., Pritchard, S. E., Placidi, E., Murray, K., Chaddock, G., Costigan, C., Lam, C., Jalanka-Tuovinen, J., De Vos, W. M., Gowland, P. A., & Spiller, R. C. (2014). Stimulation of colonic motility by oral PEG electrolyte bowel preparation assessed by MRI: comparison of split vs single dose. Neurogastroenterology and Motility, 26(10), 1426-1436. https://doi.org/10.1111/NMO.12403
  • Luo, M., Liu, Y., Nikolovska, K., Riederer, B., Patrucco, E., Hofmann, F., & Seidler, U. (2024). cGMP-dependent kinase 2, Na+/H+ exchanger NHE3, and PDZ-adaptor NHERF2 co-assemble in apical membrane microdomains. Acta Physiologica, 240(4), e14125. https://doi.org/10.1111/APHA.14125
  • Hayee, B., Watson, K. L., Campbell, S., Simpson, A., Farrell, E., Hutchings, P., Macedo, P., Perrin, F., Whelan, K., & Elston, C. (2019). A high prevalence of chronic gastrointestinal symptoms in adults with cystic fibrosis is detected using tools already validated in other GI disorders. United European Gastroenterology Journal, 7(7), 881-888. https://doi.org/10.1177/2050640619841545
  • Hannig, G., Tchernychev, B., Kurtz, C. B., Bryant, A. P., Currie, M. G., & Silos-Santiago, I. (2014). Guanylate cyclase-C/cGMP: an emerging pathway in the regulation of visceral pain. Frontiers in Molecular Neuroscience, 7(1 APR), 31. https://doi.org/10.3389/FNMOL.2014.00031
  • Ford, A. C., & Suares, N. C. (2011). Effect of laxatives and pharmacological therapies in chronic idiopathic constipation: systematic review and meta-analysis. Gut, 60(2), 209-218. https://doi.org/10.1136/GUT.2010.227132
  • Dellschaft, N., Murray, K., Ren, Y., Marciani, L., Gowland, P., Spiller, R., & Hoad, C. (2025). Assessing Water Content of the Human Colonic Chyme Using the MRI Parameter T1: A Key Biomarker of Colonic Function. Neurogastroenterology and Motility, 37(4). https://doi.org/10.1111/NMO.14999
  • Dellschaft, N., Hoad, C., Marciani, L., Gowland, P., & Spiller, R. (2022). Small bowel water content assessed by MRI in health and disease: a collation of single-centre studies. Alimentary Pharmacology & Therapeutics, 55(3), 327-338. https://doi.org/10.1111/APT.16673
  • e Lisle, R. C., & Borowitz, D. (2013). The Cystic Fibrosis Intestine. Cold Spring Harbor Perspectives in Medicine, 3(9), a009753. https://doi.org/10.1101/CSHPERSPECT.A009753
  • Corsetti, M., & Tack, J. (2013). Linaclotide: A new drug for the treatment of chronic constipation and irritable bowel syndrome with constipation. United European Gastroenterology Journal, 1(1), 7-20. https://doi.org/10.1177/2050640612474446
  • Busby, R. W., Kessler, M. M., Bartolini, W. P., Bryant, A. P., Hannig, G., Higgins, C. S., Solinga, R. M., Tobin, J. V., Wakefield, J. D., Kurtz, C. B., & Currie, M. G. (2013). Pharmacologic Properties, Metabolism, and Disposition of Linaclotide, a Novel Therapeutic Peptide Approved for the Treatment of Irritable Bowel Syndrome with Constipation and Chronic Idiopathic Constipation. The Journal of Pharmacology and Experimental Therapeutics, 344(1), 196-206. https://doi.org/10.1124/JPET.112.199430
  • Bryant, A. P., Busby, R. W., Bartolini, W. P., Cordero, E. A., Hannig, G., Kessler, M. M., Pierce, C. M., Solinga, R. M., Tobin, J. V., Mahajan-Miklos, S., Cohen, M. B., Kurtz, C. B., & Currie, M. G. (2010). Linaclotide is a potent and selective guanylate cyclase C agonist that elicits pharmacological effects locally in the gastrointestinal tract. Life Sciences, 86(19-20), 760-765. https://doi.org/10.1016/J.LFS.2010.03.015
  • Atluri, D. K., Chandar, A. K., Bharucha, A. E., & Falck-Ytter, Y. (2014). Effect of linaclotide in irritable bowel syndrome with constipation (IBS-C): a systematic review and meta-analysis. Neurogastroenterology and Motility, 26(4), 499-509. https://doi.org/10.1111/NMO.12292
  • Aliyu, A., Dellschaft, N., Hoad, C., Williams, H., Gaudoin, E., Sulaiman, S., Crooks, C., Gowland, P., Aran, A., Lange, R., Bois De Fer, B., Corsetti, M., Marciani, L., & Spiller, R. (2025). Magnetic Resonance Imaging Reveals Novel Insights into the Dual Mode of Action of Bisacodyl: A Randomized, Placebo-controlled Trial in Constipation. Clinical Pharmacology and Therapeutics, 117(5), 1284-1291. https://doi.org/10.1002/CPT.3532

Dates d'enregistrement des études

Ces dates suivent la progression des dossiers d'étude et des soumissions de résultats sommaires à ClinicalTrials.gov. Les dossiers d'étude et les résultats rapportés sont examinés par la Bibliothèque nationale de médecine (NLM) pour s'assurer qu'ils répondent à des normes de contrôle de qualité spécifiques avant d'être publiés sur le site Web public.

Dates principales de l'étude

Début de l'étude (Réel)

9 mars 2026

Achèvement primaire (Estimé)

1 août 2026

Achèvement de l'étude (Estimé)

1 octobre 2026

Dates d'inscription aux études

Première soumission

15 décembre 2025

Première soumission répondant aux critères de contrôle qualité

28 avril 2026

Première publication (Réel)

6 mai 2026

Mises à jour des dossiers d'étude

Dernière mise à jour publiée (Réel)

6 mai 2026

Dernière mise à jour soumise répondant aux critères de contrôle qualité

28 avril 2026

Dernière vérification

1 avril 2026

Plus d'information

Termes liés à cette étude

Mots clés

Termes MeSH pertinents supplémentaires

Autres numéros d'identification d'étude

  • FMHS-10-1025

Plan pour les données individuelles des participants (IPD)

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INDÉCIS

Informations sur les médicaments et les dispositifs, documents d'étude

Étudie un produit pharmaceutique réglementé par la FDA américaine

Non

Étudie un produit d'appareil réglementé par la FDA américaine

Non

produit fabriqué et exporté des États-Unis.

Non

Ces informations ont été extraites directement du site Web clinicaltrials.gov sans aucune modification. Si vous avez des demandes de modification, de suppression ou de mise à jour des détails de votre étude, veuillez contacter register@clinicaltrials.gov. Dès qu'un changement est mis en œuvre sur clinicaltrials.gov, il sera également mis à jour automatiquement sur notre site Web .

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