What Factors Affect Breast Cancer Neoadjuvant Chemotherapy Efficacy?
What Are the Factors Affecting Neoadjuvant Chemotherapy Efficacy in Breast Cancer? A Non-invasive in Vivo Study Using Specialist Magnetic Resonance (MR) Methods
Breast cancer is the most prevalent cancer affecting women. To treat locally advanced breast cancers, neoadjuvant chemotherapy (NACT) is often carried out before surgery to reduce the tumour size to allow breast conservation surgery. However, treatment response for individual patients varies, where the tumour may not respond to treatment and the quality of patient care is compromised if the NACT treatment plan is not optimised. Therefore, the assessment of NACT efficacy is beneficial for the early identification of these patients and appropriate management of treatment.
Breast tumours have unique features compared to healthy tissue, including abnormal tissue structure and biochemical composition. With NACT there are specific changes to such tumour features indicating tumour treatment response.
The purpose of this study is to establish how the changes to breast tumour features following NACT treatment are seen in non-invasive imaging. This study will look at scans of breast tumours using magnetic resonance imaging (MRI). Changes to tissue structure will be measured by advanced diffusion MRI techniques and changes to tumour related biochemical substances will be measured by advanced magnetic resonance spectroscopy techniques. The investigators aim to assess if these techniques can provide information on the tumour treatment response following subsequent rounds of NACT treatment.
In this longitudinal study, 25 patients undergoing NACT will be recruited for four repeated MRI investigations over the course of NACT treatment. Magnetic resonance (MR) measurements of tissue microstructure and biochemical composition will be compared against histological measurements and radiological assessments of treatment response.
The study will recruit patients undergoing treatment at the NHS Grampian. This research is funded by Friends of ANCHOR, Tenovus Scotland Grampian and the NHS Grampian Endowment Research Fund.
Przegląd badań
Status
Interwencja / Leczenie
Szczegółowy opis
In this single group longitudinal study, the investigators propose that functional images from magnetic resonance (MR) methods performed at baseline and after 6 cycles of neoadjuvant chemotherapy (NACT) are in agreement with histological findings from pre-treatment biopsy and post-treatment surgically excised tissue. MR methods will be performed at baseline (pre-treatment) and after the 1st, 3rd and 6th (post-treatment) cycles of NACT treatment. The investigators hypothesise that specific physiological changes detected through MR methods are a manifestation of tumour response to NACT confirmed by histology and radiological assessment (Hypothesis 1). The investigators further hypothesise that early sensitivity to physiological changes manifesting from tumour response to NACT can be revealed by MR measurements after the first and third cycle of treatment (Hypothesis 2).
Research Question 1: Is there a difference in physiological parameters revealed by MR measurements at baseline and after completion of NACT?
Research Question 2: Do the physiological measurements at the completion of NACT from MR measures agree with histological findings?
Research Question 3: Is there a difference between MR measurements at baseline and after the first and third cycle of NACT?
Research Question 4: Is there a difference in MR measurements at baseline, first and third cycle of NACT, between positive treatment responders and non-responders.
MR measurements will be compared against clinical and study specific results from histological analysis and radiological assessment of MRI, mammography and ultrasound measures of tumour treatment response. Information collected from a health questionnaire will supplement interpretation of the data.
To test the effects of NACT on specific aspects of tumour physiology, paired t-tests will be performed on MR measures of lactate concentration, lipid composition and diffusion parameters, between baseline and post-treatment assessments (Research Question 1).
To examine the relationship between MR measurements and histology, correlation analysis will be conducted between baseline and post-treatment assessments. MR measures will be correlated against corresponding percentage changes in histological findings between biopsy and tumour excision (Research Question 2).
To evaluate MR measures as early markers of NACT efficacy, paired t-tests will be carried out between MR measures at pre-treatment and post 1st and 3rd cycles of NACT treatment (Research Question 3), with independent group difference determined between responders and non-responders (Research Question 4).
Typ studiów
Zapisy (Oczekiwany)
Faza
- Nie dotyczy
Kontakty i lokalizacje
Lokalizacje studiów
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Aberdeenshire
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Aberdeen, Aberdeenshire, Zjednoczone Królestwo, AB25 2ZD
- Rekrutacyjny
- NHS Grampian
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Kontakt:
- Nicholas Senn, MPhys
- Numer telefonu: +44 (0)1224 43-8351
- E-mail: r03ns15@abdn.ac.uk
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Główny śledczy:
- Nicholas Senn, MPhys
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Kryteria uczestnictwa
Kryteria kwalifikacji
Wiek uprawniający do nauki
Akceptuje zdrowych ochotników
Płeć kwalifikująca się do nauki
Opis
Inclusion Criteria:
- Patients with pathologically confirmed invasive breast cancer undergoing neoadjuvant chemotherapy and surgery.
Exclusion Criteria:
- Condition to contradictive to MRI investigation with contrast agent (poor renal function, contrast agent allergy, metal implants or pace maker).
- Started hormone or chemotherapy treatment before recruitment.
- Undergoing treatment for concurrent cancer diagnosis.
- Marker coil contradictive to MRI investigation.
Plan studiów
Jak projektuje się badanie?
Szczegóły projektu
- Główny cel: Podstawowa nauka
- Przydział: Nie dotyczy
- Model interwencyjny: Zadanie dla jednej grupy
- Maskowanie: Brak (otwarta etykieta)
Broń i interwencje
Grupa uczestników / Arm |
Interwencja / Leczenie |
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Eksperymentalny: Single Arm
25 patients with pathologically confirmed invasive breast cancer who will undergo neoadjuvant chemotherapy treatment (NACT) and surgery will be recruited.
During the study, patients will receive the standard care and the current study will not alter the care plan offered to them.
All patients in the single arm will undergo 4 magnetic resonance imaging scan sessions.
Histopathological analysis will be performed on the core biopsy and tumour tissue removed in surgery.
Health questionnaire will be completed by each patient.
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Patients will undergo 4 MRI sessions during NACT treatment.
The first scan will take place at treatment baseline before their first cycle of NACT treatment.
The second scan will take place following the first treatment cycle prior to the second treatment cycle.
Likewise, the third and last scan will take place after the third treatment cycle and sixth (final) treatment cycle prior to surgery.
MRI scan sessions will be composed of research scans including diffusion and lipid profiling MR imaging methods and MR spectroscopy (MRS) methods.
Study specific analysis will be performed on the core biopsy and tissue removed in surgery following the completion of NACT treatment.
Standard routine histological analysis will be performed, as well as study specific analysis for immunostaining, grading and slide scan imaging for measurement of cellularity markers.
Health and demographic information will be collected.
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Co mierzy badanie?
Podstawowe miary wyniku
Miara wyniku |
Opis środka |
Ramy czasowe |
|---|---|---|
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Baseline: Water diffusion probability density function (Full-Width-At-Half-Maximum, FWHM, units of micrometre)
Ramy czasowe: Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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The water diffusion probability density function will be quantified by the Full-Width-At-Half-Maximum with units of micrometre
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Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Post Cycle 1: Water diffusion probability density function (Full-Width-At-Half-Maximum, FWHM, units of micrometre)
Ramy czasowe: Scan at the end of Cycle 1 (Each cycle is 21 days)
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The water diffusion probability density function will be quantified by the Full-Width-At-Half-Maximum with units of micrometre
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Scan at the end of Cycle 1 (Each cycle is 21 days)
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Post Cycle 3: Water diffusion probability density function (Full-Width-At-Half-Maximum, FWHM, units of micrometre)
Ramy czasowe: Scan at the end of Cycle 3 (Each cycle is 21 days)
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The water diffusion probability density function will be quantified by the Full-Width-At-Half-Maximum with units of micrometre
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Scan at the end of Cycle 3 (Each cycle is 21 days)
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Post Treatment: Water diffusion probability density function (Full-Width-At-Half-Maximum, FWHM, units of micrometre)
Ramy czasowe: Scan at the end of Cycle 6 (Each cycle is 21 days)
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The water diffusion probability density function will be quantified by the Full-Width-At-Half-Maximum with units of micrometre
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Scan at the end of Cycle 6 (Each cycle is 21 days)
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Baseline: Water diffusivity (units of mm^2 /s)
Ramy czasowe: Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Water diffusivity with units of mm^2 /s
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Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Post Cycle 1: Water diffusivity (units of mm^2 /s)
Ramy czasowe: Scan at the end of Cycle 1 (Each cycle is 21 days)
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Water diffusivity with units of mm^2 /s
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Scan at the end of Cycle 1 (Each cycle is 21 days)
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Post Cycle 3: Water diffusivity (units of mm^2 /s)
Ramy czasowe: Scan at the end of Cycle 3 (Each cycle is 21 days)
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Water diffusivity with units of mm^2 /s
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Scan at the end of Cycle 3 (Each cycle is 21 days)
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Post Treatment: Water diffusivity (units of mm^2 /s)
Ramy czasowe: Scan at the end of Cycle 6 (Each cycle is 21 days)
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Water diffusivity with units of mm^2 /s
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Scan at the end of Cycle 6 (Each cycle is 21 days)
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Baseline: Lactate Concentration (units of mM)
Ramy czasowe: Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Lactate concentration with units of mM
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Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Post Cycle 1: Lactate Concentration (units of mM)
Ramy czasowe: Scan at the end of Cycle 1 (Each cycle is 21 days)
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Lactate concentration with units of mM
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Scan at the end of Cycle 1 (Each cycle is 21 days)
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Post Cycle 3: Lactate Concentration (units of mM)
Ramy czasowe: Scan at the end of Cycle 3 (Each cycle is 21 days)
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Lactate concentration with units of mM
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Scan at the end of Cycle 3 (Each cycle is 21 days)
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Post Treatment: Lactate Concentration (units of mM)
Ramy czasowe: Scan at the end of Cycle 6 (Each cycle is 21 days)
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Lactate concentration with units of mM
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Scan at the end of Cycle 6 (Each cycle is 21 days)
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Baseline: Lipid Peak Volume Ratio (Ratio Units)
Ramy czasowe: Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Lipid peak volume ratio value with units of ratio
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Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Post Cycle 1: Lipid Peak Volume Ratio (Ratio Units)
Ramy czasowe: Scan at the end of Cycle 1 (Each cycle is 21 days)
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Lipid peak volume ratio value with units of ratio
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Scan at the end of Cycle 1 (Each cycle is 21 days)
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Post Cycle 3: Lipid Peak Volume Ratio (Ratio Units)
Ramy czasowe: Scan at the end of Cycle 3 (Each cycle is 21 days)
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Lipid peak volume ratio value with units of ratio
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Scan at the end of Cycle 3 (Each cycle is 21 days)
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Post Treatment: Lipid Peak Volume Ratio (Ratio Units)
Ramy czasowe: Scan at the end of Cycle 6 (Each cycle is 21 days)
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Lipid peak volume ratio value with units of ratio
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Scan at the end of Cycle 6 (Each cycle is 21 days)
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Baseline: Fat Fraction (units of %)
Ramy czasowe: Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Fat Fraction with units of %
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Scan at pre-treatment baseline (Prior to start of Cycle 1, each cycle is 21 days)
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Post Cycle 1: Fat Fraction (units of %)
Ramy czasowe: Scan at the end of Cycle 1 (Each cycle is 21 days)
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Fat Fraction with units of %
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Scan at the end of Cycle 1 (Each cycle is 21 days)
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Post Cycle 3: Fat Fraction (units of %)
Ramy czasowe: Scan at the end of Cycle 3 (Each cycle is 21 days)
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Fat Fraction with units of %
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Scan at the end of Cycle 3 (Each cycle is 21 days)
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Post Treatment: Fat Fraction (units of %)
Ramy czasowe: Scan at the end of Cycle 6 (Each cycle is 21 days)
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Fat Fraction with units of %
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Scan at the end of Cycle 6 (Each cycle is 21 days)
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Miary wyników drugorzędnych
Miara wyniku |
Opis środka |
Ramy czasowe |
|---|---|---|
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Core Biopsy Tumour Tissue: Ki-67 Staining Percentage (units of %)
Ramy czasowe: Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Ki-67 staining percentage with units of %, assessed on core biopsy tissue taken prior to start of treatment cycles.
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Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Excised Tumour Tissue: Ki-67 Staining Percentage (units of %)
Ramy czasowe: Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Ki-67 staining percentage with units of %, assessed on excised tissue taken from surgery following completion of treatment cycles.
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Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Core Biopsy Tumour Tissue: Serotonin Staining Score (arbitrary units)
Ramy czasowe: Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Serotonin staining score with arbitrary units (multiplication of staining percentage and stain intensity scored 1 - 3), assessed on core biopsy tissue taken prior to start of treatment cycles.
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Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Excised Tumour Tissue: Serotonin Staining Score (arbitrary units)
Ramy czasowe: Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Serotonin staining score with arbitrary units (multiplication of staining percentage and stain intensity scored 1 - 3), assessed on excised tissue taken from surgery following completion of treatment cycles.
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Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Core Biopsy Tumour Tissue: Cellularity (units of %)
Ramy czasowe: Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Cellularity with units of %, assessed on core biopsy taken prior to start of treatment cycles.
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Pre-treatment baseline biopsy (Taken prior to start of Cycle 1, each cycle is 21 days). Assessed following completion of treatment cycles and the routine reporting of core biopsy and excised tissue samples.
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Excised Tumour Tissue: Cellularity (units of %)
Ramy czasowe: Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Cellularity with units of %, assessed on excised tissue taken from surgery following completion of treatment cycles.
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Post-treatment surgery excision (Post Cycle 6, each cycle is 21 days). Assessed following the completion of routine pathological reporting of the excised tissue.
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Współpracownicy i badacze
Sponsor
Współpracownicy
Śledczy
- Krzesło do nauki: Jiabao He, PhD, University of Aberdeen
- Główny śledczy: Nicholas Senn, MPhys, University of Aberdeen
Publikacje i pomocne linki
Publikacje ogólne
- Graham LJ, Shupe MP, Schneble EJ, Flynt FL, Clemenshaw MN, Kirkpatrick AD, Gallagher C, Nissan A, Henry L, Stojadinovic A, Peoples GE, Shumway NM. Current approaches and challenges in monitoring treatment responses in breast cancer. J Cancer. 2014 Jan 5;5(1):58-68. doi: 10.7150/jca.7047.
- Naressi A, Couturier C, Castang I, de Beer R, Graveron-Demilly D. Java-based graphical user interface for MRUI, a software package for quantitation of in vivo/medical magnetic resonance spectroscopy signals. Comput Biol Med. 2001 Jul;31(4):269-86. doi: 10.1016/s0010-4825(01)00006-3.
- Lee MC, Newman LA. Management of patients with locally advanced breast cancer. Surg Clin North Am. 2007 Apr;87(2):379-98, ix. doi: 10.1016/j.suc.2007.01.012.
- Luangdilok S, Samarnthai N, Korphaisarn K. Association between Pathological Complete Response and Outcome Following Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer Patients. J Breast Cancer. 2014 Dec;17(4):376-85. doi: 10.4048/jbc.2014.17.4.376. Epub 2014 Dec 26.
- Danishad KK, Sharma U, Sah RG, Seenu V, Parshad R, Jagannathan NR. Assessment of therapeutic response of locally advanced breast cancer (LABC) patients undergoing neoadjuvant chemotherapy (NACT) monitored using sequential magnetic resonance spectroscopic imaging (MRSI). NMR Biomed. 2010 Apr;23(3):233-41. doi: 10.1002/nbm.1436.
- Woodhams R, Kakita S, Hata H, Iwabuchi K, Kuranami M, Gautam S, Hatabu H, Kan S, Mountford C. Identification of residual breast carcinoma following neoadjuvant chemotherapy: diffusion-weighted imaging--comparison with contrast-enhanced MR imaging and pathologic findings. Radiology. 2010 Feb;254(2):357-66. doi: 10.1148/radiol.2542090405.
- Walenta S, Mueller-Klieser WF. Lactate: mirror and motor of tumor malignancy. Semin Radiat Oncol. 2004 Jul;14(3):267-74. doi: 10.1016/j.semradonc.2004.04.004.
- Carmona-Fontaine C, Bucci V, Akkari L, Deforet M, Joyce JA, Xavier JB. Emergence of spatial structure in the tumor microenvironment due to the Warburg effect. Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):19402-7. doi: 10.1073/pnas.1311939110. Epub 2013 Nov 11.
- Bolan PJ, Meisamy S, Baker EH, Lin J, Emory T, Nelson M, Everson LI, Yee D, Garwood M. In vivo quantification of choline compounds in the breast with 1H MR spectroscopy. Magn Reson Med. 2003 Dec;50(6):1134-43. doi: 10.1002/mrm.10654.
- Baik HM, Su MY, Yu H, Mehta R, Nalcioglu O. Quantification of choline-containing compounds in malignant breast tumors by 1H MR spectroscopy using water as an internal reference at 1.5 T. MAGMA. 2006 May;19(2):96-104. doi: 10.1007/s10334-006-0032-4. Epub 2006 May 9.
- He Q, Shkarin P, Hooley RJ, Lannin DR, Weinreb JC, Bossuyt VI. In vivo MR spectroscopic imaging of polyunsaturated fatty acids (PUFA) in healthy and cancerous breast tissues by selective multiple-quantum coherence transfer (Sel-MQC): a preliminary study. Magn Reson Med. 2007 Dec;58(6):1079-85. doi: 10.1002/mrm.21335.
- Yamada I, Hikishima K, Miyasaka N, Tokairin Y, Ito E, Kawano T, Kobayashi D, Eishi Y, Okano H. Esophageal carcinoma: Evaluation with q-space diffusion-weighted MR imaging ex vivo. Magn Reson Med. 2015 Jun;73(6):2262-73. doi: 10.1002/mrm.25334. Epub 2014 Jun 19.
Daty zapisu na studia
Główne daty studiów
Rozpoczęcie studiów (Rzeczywisty)
Zakończenie podstawowe (Oczekiwany)
Ukończenie studiów (Oczekiwany)
Daty rejestracji na studia
Pierwszy przesłany
Pierwszy przesłany, który spełnia kryteria kontroli jakości
Pierwszy wysłany (Rzeczywisty)
Aktualizacje rekordów badań
Ostatnia wysłana aktualizacja (Rzeczywisty)
Ostatnia przesłana aktualizacja, która spełniała kryteria kontroli jakości
Ostatnia weryfikacja
Więcej informacji
Terminy związane z tym badaniem
Dodatkowe istotne warunki MeSH
Inne numery identyfikacyjne badania
- 2/108/7
- 234794 (Inny identyfikator: Integrated Research Approval System project ID)
- 17/LO/1777 (Inny identyfikator: Research Ethics Committee Reference)
Plan dla danych uczestnika indywidualnego (IPD)
Planujesz udostępniać dane poszczególnych uczestników (IPD)?
Informacje o lekach i urządzeniach, dokumenty badawcze
Bada produkt leczniczy regulowany przez amerykańską FDA
Bada produkt urządzenia regulowany przez amerykańską FDA
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