Comparison of Hematopoietic Stem Cell Activity in Adipose Tissue From Type 2 Diabetic Patients and Healthy Volunteers (WAT2DO)

Inclusion Criteria:

• No major weight variation for at least 3 months
• Biological assessment without clinically significant anomaly from the point of view of the investigator.
• Acceptance of constraints related to participation in the study
• Acceptance of participation in the constitution of a cell bank, a tissue bank and a serum library.
• Affiliation to a social security scheme.

Type 2 diabetic subjects:

• Type 2 diabetes (discovered after the age of 35 years, without inaugural ketosis and absence of insulin therapy during the first year).
• 40 to 60 year-old.
• BMI between 27 and 35 kg / m² (included).
• Treated by modification of lifestyle alone or associated to oral anti-diabetic therapy only.
• With stable oral anti-diabetic treatment for at least 3 months.
• HbA1c ≤ 8.5%.

Healthy Volunteers:

• BMI between 23 and 27 kg / m² (included).
• 37 to 63 year-old, age-matched to a type 2 diabetes subject ± 5 years.
• Fasting blood glucose < 1,10 g / L.
• HbA1c within normal limits (4 to 6%).

Exclusion Criteria:

• Excessive chronic alcohol consumption (> 30 g / day or 210 g / week).
• Tobacco consumption> 10 cigarettes / day that cannot be stopped for 24 hours.
• Anti-diabetic treatments that require sub-cutaneous injections
• History of chronic or acute hematological pathology.
• Systemic or acute inflammatory pathology.
• Treatment with antiplatelet agents, non-steroidal anti-inflammatory drugs, glucocorticoids (excluding eye drops and sprays), or other immunosuppressive drugs.
• History of cancer (except basal cell carcinoma).
• Allergy to xylocaine or one of its derivatives.
• Any significant pathology at the discretion of the investigator.
• Any biological anomaly at the discretion of the investigator.
• Positive human immunodeficiency virus serology.
• Positive hepatitis B serology.
• Positive hepatitis C serology.
• Glomerular filtration rate less than 60 ml / min
• Aspartate aminotransferase or alanine aminotransferase higher than 2.5-fold the upper normal value.
• Hypertriglyceridemia > 2.5 g / l
• Person under the protection of justice, guardianship or curators.
• Subject involved in another research protocol or in an exclusion period from another research protocol.
• Cognitive disorder or mental pathology (at the discretion of the investigator).