DNA Methylation and 1-C Metabolism Nutrient Intake

Dietary Intake of One-carbon Metabolism Nutrients and DNA Methylation in Peripheral Blood

The present study used a sub-sample of the Melbourne Collaborative Cohort Study (MCCS) (Milne RL et al, Int J Epidemiol, 2017), who had valid data on dietary intakes (121-item food frequency questionnaire) and valid measures of DNA methylation using the HumanMethylation Infinium 450K Beadchip assay, or using LINE-1, Alu and Sat2 assays.

The investigators studied the association between nutrients involved in one-carbon metabolism and DNA methylation in peripheral blood.

Study Overview

• Status: Completed
• Conditions: DNA Methylation

• Study Type: Observational
• Enrollment (Actual): 5983

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 27 years to 80 years (ADULT, OLDER_ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

• Sampling Method: Non-Probability Sample

Study Population

The population is a sub-sample of the Melbourne Collaborative Cohort Study (MCCS), with non-random sampling based on selection into of six case-control studies nested within the MCCS. The MCCS is a prospective cohort study of 41,513 people (17,044 men and 24,469 women) recruited between 1990 and 1994. The age range at recruitment was 27-80 (99.3% being aged 40-69). Southern European migrants to Australia were over-sampled to extend the range of exposures, including dietary patterns.

Participants in this sub-study had valid dietary intake data and valid measures of DNA methylation.

Description

Inclusion Criteria:

• Selected into one of six case-control studies nested in the Melbourne Collaborative Cohort Study
• Completed a 121-item Food Frequency Questionnaire
• Had a blood DNA sample with a measure of methylation

Exclusion Criteria:

• Missing data in any of the outcome, exposure, or confounders (N=797 excluded)

Study Plan

How is the study designed?

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Blood DNA methylation	Methylation of DNA from peripheral blood, HumanMethylation 450 Beadchip assay	2014-2015

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Blood DNA methylation	Methylation of DNA from peripheral blood, LINE-1, Alu, Sat2	2014-2015

Collaborators and Investigators

• Sponsor: Cancer Council Victoria
• Investigators: Principal Investigator: Graham G Giles, PhD, Cancer Council Victoria

Publications and helpful links

General Publications

• Chamberlain JA, Dugue PA, Bassett JK, Hodge AM, Brinkman MT, Joo JE, Jung CH, Makalic E, Schmidt DF, Hopper JL, Buchanan DD, English DR, Southey MC, Giles GG, Milne RL. Dietary intake of one-carbon metabolism nutrients and DNA methylation in peripheral blood. Am J Clin Nutr. 2018 Sep 1;108(3):611-621. doi: 10.1093/ajcn/nqy119.

Study record dates

Study Major Dates

• Study Start (ACTUAL): January 1, 2014
• Primary Completion (ACTUAL): December 31, 2015
• Study Completion (ACTUAL): December 31, 2015

Study Registration Dates

• First Submitted: July 21, 2017
• First Submitted That Met QC Criteria: July 21, 2017
• First Posted (ACTUAL): July 24, 2017

Study Record Updates

• Last Update Posted (ACTUAL): July 24, 2017
• Last Update Submitted That Met QC Criteria: July 21, 2017
• Last Verified: July 1, 2017

More Information

Terms related to this study

• Other Study ID Numbers: CEID_methylation_1

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No