- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT03552081
Tobacco and Sperm Genome: Effects of Smoking Cessation (TABAGERM)
Study Overview
Status
Conditions
Intervention / Treatment
Detailed Description
Tobacco is a male infertility risk factor. Many studies comparing smokers to non-smokers have shown effects on spermatogenesis and the quality of the male gamete. In vitro fertilization success rates are decreased in smokers and in natural procreation the time required to conceive (excellent fertility indicator) seems to be lengthened in the smoker. Tobacco is a well-known mutagen. It will induce damage to the male gametes DNA. Tobacco increases the oxydation of DNA. Exposure to tobacco increases the percentage of spermatozoa with broken DNA or defective chromatin and induces gametes aneuploidy as has been reported in several studies. The set of effects of tobacco on the male gametes questions the consequences for the conceptus (from embryo to adult). The prospective study that the investigators propose to carry out would make it possible to know the time required for the repair of the sperm abnormalities and in particular the DNA of the gametes generated by the smoking. Indeed, a cycle of spermatogenesis extending over 74 days, it would be important to know if it is necessary to wait a complete cycle or even more before proposing a management. It would therefore be possible to find the most favorable period for reducing tobacco-related risks and for effective management.
A blood test will be performed at T0 (before weaning) and during follow-up at 3, 6 and 12 months for the determination of the Inhibin hormones and testosterone. Sperm analysis at the different follow-up times will be carried out at CECOS using a standardized methodology (WHO). Two tests of analysis will be used in this study on each sample: the TUNEL test and the SCSA. A measurement of DNA oxidation will also be performed, as well as a study of spermatic aneuploidy (in situ hybridization technique of fluorescent centromeric chromosomal probes).
Study Type
Enrollment (Actual)
Phase
- Not Applicable
Contacts and Locations
Study Locations
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Toulouse, France
- CHU Toulouse, Service de médecine de la reproduction
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Participation Criteria
Eligibility Criteria
Ages Eligible for Study
Accepts Healthy Volunteers
Genders Eligible for Study
Description
Inclusion Criteria:
- 1st Consultation in the Tobacco Control Co-ordination Unit,
- Affiliate or beneficiary of a social security scheme
- Free, informed and written consent signed by the participant and the investigator
Exclusion Criteria:
- febrile state during the 3 months before inclusion,
- antecedent consultation for male infertility,
- BMI> 30,
- Medical and / or surgical history that can alter spermatogenesis -Chemotherapy / Radiation Therapy / IRA-therapy, Cryptorchidism, testicular Torsion, significant varicocele, chronic, progressive disease,
- hypovolemia <1.6ml
- Major persons protected by law (guardianship, curators, safeguarding of justice)
- Patients in periods of exclusion for another clinical research protocol
- Co-addiction (cannabis, alcohol, other drugs)
- Neuropsychiatric Pathology, considered serious by the investigator
- Psychotropic treatment (antidepressant, anxiolytic, antipsychotic)
Study Plan
How is the study designed?
Design Details
- Primary Purpose: Other
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
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Experimental: fragmented DNA evaluation in blood and semen samples
20 men followed for smoking cessation will be included in the study in order to evaluate the time required for the repair of the sperm abnormalities and in particular the DNA of the gametes generated by the smoking
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The objective will be to evaluate the decrease in the percentage of sperm with spermatic DNA fragmentation by the TUNEL technique following the evolution of this rate to 3 months, 6 months and 12 months after cessation of smoking by blood and semen samples.
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What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Change of the Percentage of spermatozoa presenting a fragmented DNA from Baseline to 12 months after smoking cessation
Time Frame: Up to 12 months after smoking cessation
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TUNEL technique will be performed to evaluate the percentage of spermatozoa presenting a fragmented DNA at day 0 (before weaning = baseline), and 12 months after smoking cessation
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Up to 12 months after smoking cessation
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Change of the Percentage of spermatozoa presenting a fragmented DNA from Baseline to 3 months after smoking cessation
Time Frame: Up to 3 months after smoking cessation
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TUNEL technique will be performed to evaluate the percentage of spermatozoa presenting a fragmented DNA at day 0 (before weaning = baseline), and 3 months after smoking cessation
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Up to 3 months after smoking cessation
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Change of the Percentage of spermatozoa presenting a fragmented DNA from Baseline to 6 months after smoking cessation
Time Frame: Up to 6 months after smoking cessation
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TUNEL technique will be performed to evaluate the percentage of spermatozoa presenting a fragmented DNA at day 0 (before weaning = baseline), and 6 months after smoking cessation
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Up to 6 months after smoking cessation
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Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Rate of 8-OHdG gamete DNA
Time Frame: Up to 12 months after smoking cessation
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Up to 12 months after smoking cessation
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Spermogram exam
Time Frame: Up to 12 months after smoking cessation
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Spermogram examination
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Up to 12 months after smoking cessation
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Fragmentation index (DFI) of gamete DNA
Time Frame: Up to 12 months after smoking cessation
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Up to 12 months after smoking cessation
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Aneuploid sperm count
Time Frame: Up to 12 months after smoking cessation
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Up to 12 months after smoking cessation
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luteinizing hormone (LH) levels luteinizing hormone (LH) levels luteinizing hormone (LH) levels
Time Frame: Up to 12 months after smoking cessation
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Blood samples at day 0 (before weaning), and at 3 months, 6 months, 12 months after smoking cessation
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Up to 12 months after smoking cessation
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Testosterone levels
Time Frame: Up to 12 months after smoking cessation
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Blood samples at day 0 (before weaning), and at 3 months, 6 months, 12 months after smoking cessation
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Up to 12 months after smoking cessation
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Collaborators and Investigators
Sponsor
Investigators
- Principal Investigator: Jessica Moreau, MD, Chu Toulouse
Study record dates
Study Major Dates
Study Start (Actual)
Primary Completion (Actual)
Study Completion (Actual)
Study Registration Dates
First Submitted
First Submitted That Met QC Criteria
First Posted (Actual)
Study Record Updates
Last Update Posted (Actual)
Last Update Submitted That Met QC Criteria
Last Verified
More Information
Terms related to this study
Additional Relevant MeSH Terms
Other Study ID Numbers
- RC31/17/0344
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
Studies a U.S. FDA-regulated device product
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
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