- ICH GCP
- US Clinical Trials Registry
- Clinical Trial NCT04823429
HIIT and MICT on Nitric Oxide-mediated Erythrocyte Rheology
March 26, 2021 updated by: Jong-Shyan Wang, Chang Gung Memorial Hospital
Effects of High Intensity Interval Training and Moderate Intensity Continuous Training on Nitric Oxide-mediated Erythrocyte Rheology
Erythrocyte rheological properties affect blood viscoelasticity and consequently regulate vascular resistance to flow shear force, whereas rheological impairments of erythrocytes may result in circulatory disorders.
The aim of this study was to establish an effective exercise strategy for improving individual aerobic capacity and for simultaneously ameliorating the risk of hemorheological dysfunction evoked by a graded exercise test (GXT) and the hypotheses is exercise intervention will improved hemorheological functions by enhancing deformability of erythrocytes via NO-mediated mechanism.
This study included 60 healthy sedentary mens (age 20~30) from Chang Gung university than were randomized into the HIIT [3-min intervals at 40% and 80% V̇O2 reserve (V̇O2R),n=10] and MICT(sustained 60% V̇O2R,n=10)on a bicycle ergometer for 30min·d-1, 5 d·wk-1 for 6 wk.
Study Overview
Status
Unknown
Conditions
Intervention / Treatment
Detailed Description
Recently, the role of erythrocyte has been more emphasized, which also related with endothelial cell.
For coronary artery patients, the endothelial nitric oxide synthase activity in red blood cell (RBC-eNOS activity) is lower than age-matched health people, and it is related with dysfunction of endothelial cell.
In cardiovascular diseases.
the erythrocyte arginase-1 is active and seize L-arginine with eNOS.
When the Arg-1 stimulated by reactive oxygen species (ROS), the nitric oxide (NO) bioactivity decrease and produce more ROS, meanwhile, ROS can go around to stimulate Arg-1.
When the RBC-NO production is lowering, it will increase the adhesion activity to endothelial cell due to erythrocyte can be quite close to blood vessel well then release Nitric Oxide, induce the dysfunction and oxidative pressure of endothelial cell.
The NO can also regulate the deformability of erythrocytes, and extremely affect oxygen supply to tissue once the deformability and aggregation of erythrocyte become abnormal.
Besides NO, the deformability will be affected if erythrocyte is continuously exposed to the endogenous or exogenous ROS, which also increase adhesion to endothelial cell with the exposure of phosphatidylserine.
Exercise can regulate the mechanism of NO release from erythrocyte, affecting the rheology of erythrocyte, and improve the anti-oxidation ability.
Therefore, as mentioned above which make erythrocyte, as many aspects, become an important role on atherosclerosis disease treatment.
Study Type
Interventional
Enrollment (Anticipated)
60
Phase
- Not Applicable
Contacts and Locations
This section provides the contact details for those conducting the study, and information on where this study is being conducted.
Study Locations
-
-
-
Taoyuan, Taiwan, 333
- Recruiting
- Chang Gung University
-
-
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
20 years to 30 years (Adult)
Accepts Healthy Volunteers
Yes
Genders Eligible for Study
Male
Description
Inclusion Criteria:
- Having a sedentary lifestyle (without regular exercise, exercise frequency ≤ once weekly, duration < 20 min).
Exclusion Criteria:
- Exposed to high altitudes (> 3000 m) for at least 1 year.
- Smoker
- Taking medications or vitamins
- Having any cardiopulmonary/hematological risk.
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Treatment
- Allocation: Randomized
- Interventional Model: Parallel Assignment
- Masking: Single
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
---|---|
Experimental: High intensity-interval training (HIIT)
Subjects performed HIIT (3-min intervals at 40% and 80%VO2peak) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
|
Subjects performed HIIT (3-min intervals at 40% and 80%VO2peak) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks. Without any exercise training |
No Intervention: Control group
Without any exercise training
|
|
Experimental: Moderate intensity-continuous (MICT)
Subjects performed MICT (sustained 60%VO 2max) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
|
Subjects performed MICT (sustained 60%VO 2max) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Intracellular NO production response to exercise training.
Time Frame: 8 weeks
|
Added different inhibitors or agonists to investigate the effects of exercise training on NO production mediated by eNOS-NO pathway.
|
8 weeks
|
Intracellular ROS production response to exercise training.
Time Frame: 8 weeks
|
Added different inhibitors or agonists to investigate the effects of exercise training on ROS production mediated by eNOS-NO pathway.
|
8 weeks
|
The levels of eNOS, p-eNOS and Band-3 response to exercise training.
Time Frame: 8 weeks
|
Detect the following protein levels: eNOS, p-eNOS and Band-3, by the western blots.
|
8 weeks
|
Secondary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
---|---|---|
Determination of erythrocyte biological markers by Flow Cytometry
Time Frame: 8 weeks
|
Using the Flow Cytometry to detect the following markers: CD242, CD239, NADPH oxidase 2 (Nox2) and Arginase 1 (Arg1).
|
8 weeks
|
Erythrocyte deformability
Time Frame: 8 weeks
|
Isolated erythrocyte first, then assess erythrocyte deformability (elongation index ) by using laser assisted optical rotational red cell analyzer (LoRRca).
|
8 weeks
|
Cardiopulmonary fitness
Time Frame: 8 weeks
|
To assess cardiopulmonary fitness, cardiopulmonary exercise test (CPET) on a cycle ergometer was performed 4 days before and after the intervention.
All subjects underwent exercise with a mask to measured oxygen consumption (VO2) breath by breath using a computer-based system.
|
8 weeks
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Sponsor
Publications and helpful links
The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.
General Publications
- Mao TY, Fu LL, Wang JS. Hypoxic exercise training causes erythrocyte senescence and rheological dysfunction by depressed Gardos channel activity. J Appl Physiol (1985). 2011 Aug;111(2):382-91. doi: 10.1152/japplphysiol.00096.2011. Epub 2011 May 5.
- Agre P, King LS, Yasui M, Guggino WB, Ottersen OP, Fujiyoshi Y, Engel A, Nielsen S. Aquaporin water channels--from atomic structure to clinical medicine. J Physiol. 2002 Jul 1;542(Pt 1):3-16. doi: 10.1113/jphysiol.2002.020818.
- Wang JS, Fu TC, Lien HY, Wang CH, Hsu CC, Wu WC, Chien YW, Cherng WJ. Effect of aerobic interval training on erythrocyte rheological and hemodynamic functions in heart failure patients with anemia. Int J Cardiol. 2013 Sep 30;168(2):1243-50. doi: 10.1016/j.ijcard.2012.11.053. Epub 2012 Nov 27.
- Chou SL, Huang YC, Fu TC, Hsu CC, Wang JS. Cycling Exercise Training Alleviates Hypoxia-Impaired Erythrocyte Rheology. Med Sci Sports Exerc. 2016 Jan;48(1):57-65. doi: 10.1249/MSS.0000000000000730.
- Grau M, Pauly S, Ali J, Walpurgis K, Thevis M, Bloch W, Suhr F. RBC-NOS-dependent S-nitrosylation of cytoskeletal proteins improves RBC deformability. PLoS One. 2013;8(2):e56759. doi: 10.1371/journal.pone.0056759. Epub 2013 Feb 12.
- Suhr F, Brenig J, Muller R, Behrens H, Bloch W, Grau M. Moderate exercise promotes human RBC-NOS activity, NO production and deformability through Akt kinase pathway. PLoS One. 2012;7(9):e45982. doi: 10.1371/journal.pone.0045982. Epub 2012 Sep 25.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Actual)
March 5, 2021
Primary Completion (Anticipated)
September 4, 2021
Study Completion (Anticipated)
September 4, 2021
Study Registration Dates
First Submitted
February 26, 2021
First Submitted That Met QC Criteria
March 26, 2021
First Posted (Actual)
March 30, 2021
Study Record Updates
Last Update Posted (Actual)
March 30, 2021
Last Update Submitted That Met QC Criteria
March 26, 2021
Last Verified
March 1, 2021
More Information
Terms related to this study
Other Study ID Numbers
- 202000448A3
Plan for Individual participant data (IPD)
Plan to Share Individual Participant Data (IPD)?
NO
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.
Clinical Trials on Exercise
-
Faculdade de Motricidade HumanaCompletedGreen Exercise | Indoor ExercisePortugal
-
Hasan Kalyoncu UniversityNot yet recruiting
-
University of HawaiiKlein Buendel, Inc.CompletedMomZing Exercise Videos Online | Standard Exercise DVDUnited States
-
Istanbul Medipol University HospitalCompletedPulmonary Rehabilitation | Exercise Capacity | Exercise TestTurkey
-
University Hospital MuensterGerman Federal Ministry of Education and ResearchUnknownAerobic Exercise | Anaerobic Exercise | Unchanged ConditionGermany
-
Paracelsus Medical UniversityDepartment of Psychiatry University of BonnRecruitingHealthy | Exercise | Physical Fitness | Exercise TestAustria
-
Nathaniel JenkinsCompletedExercise Training | Physical Exertion | Desire to ExerciseUnited States
-
University of GeorgiaRecruitingBiomarkers | Aerobic Exercise | Acute ExerciseUnited States
-
University of TaipeiCompletedAerobic Exercise | Massage | Anaerobic Exercise
-
University of Alberta, Physical EducationCompletedSedentary Behaviour | Adherence | Traditional Exercise | Lifestyle ExerciseCanada
Clinical Trials on Moderate intensity-continuous (MICT)
-
Chang Gung Memorial HospitalNational Science Council, Taiwan; Chang Gung UniversityCompletedHigh Intensity Interval Training | Healthy | Exercise TrainingTaiwan
-
Guangdong Provincial People's HospitalRecruiting
-
Ottawa Heart Institute Research CorporationRecruitingAtrial Fibrillation | Paroxysmal Atrial Fibrillation | Persistent Atrial FibrillationCanada
-
Guangdong Provincial People's HospitalRecruitingMyocardial Infarction | Cardiopulmonary Disease | Training Group, SensitivityChina
-
University of Colorado, BoulderCompleted
-
University of ÉvoraCompletedPatients With Coronary Artery DiseasePortugal
-
Norwegian University of Science and TechnologyThe University of QueenslandCompleted
-
Cerini TamaraSchulthess KlinikCompleted
-
Ottawa Heart Institute Research CorporationRecruiting
-
University of KielFachklinik Satteldüne der DRV Nord; University of Kiel, Arbeitsbereich Sportmedizin... and other collaboratorsRecruitingCystic FibrosisGermany