Rehydration With a Trace Mineral Supplement (TRACE)

It is well established that post-exercise rehydration with electrolyte solutions is more effective than plain water. However, most commercially available drinks are high in electrolytes but lack some essential trace minerals. This study aims to examine the impact of a higher-electrolyte sports drink compared with a low-electrolyte, trace-mineral-rich solution and water on the time course and extent of rehydration after exercise-induced dehydration.

Study Overview

• Status: Completed
• Conditions: Dehydration (Physiology)
• Intervention / Treatment: Dietary supplement: ConcenTrace; Dietary supplement: ZeroLyte; Dietary supplement: Placebo

Detailed Description

Protocol Before the start of the study eligible participants will visit our lab to conduct a screening of medical history, weight, height, and a onetime assessment of body composition via BIA and Training history. This visit will take place at least one day before the actual start of the study and typically lasts no longer than 30 minutes. During this visit participants will be given two questionnaires, one regarding the physical activity readiness of the participant, and another one regarding their health history. If a participant does not meet the physical criteria and does not pass both questionnaires, they will be given a $25 digital Tango gift card for their brief visit to our lab. Participants who do pass will be given a scheduled date and time to visit the lab again for their first official trial.

At the start of the study subjects will visit the lab after at least 10h of fast and they will consume a light breakfast. An intravenous catheter will be place in an antecubital vein and a blood sample will be taken after 20 min for baseline. A baseline skin hydration reading will also be taken at this time. Subjects will void their bladders and a body weight will be taken.

Dehydration:

Consistent with the study by Shirreffs et al (2007) and based on our previous work, dehydration will be accomplished on the morning of testing via mild exercise and heat exposure to elicit a body weight loss of -2%. The subjects will perform four bouts of 25-minutes low-intensity exercise and 5 min rest (alternating cycling & walking) or till they reach -2% of body weight dehydration.

Rehydration:

Upon losing 2% body weight, participants will be instructe to rinse off their face to get rid of excess sweat. This is followed by a 20 minutes rest period in a thermo-comfortable environment, a dehydrated baseline blood sample will be taken, along with a dehydrated skin hydration reading and subject will start the rehydration protocol.

The volunteers will consume one of the three drinks below in a cross over balance mode. During the first hour four equal doses will be consumed in15-minutes intervals at a total volume of 150% of their body weight loss.

Trials/Drinks (block randomization will be used to assign individual participants to each specific trial/drink using their ID number):

• Water
• ConcenTrace®
• ZEROLyte™ Note: The beverage volumes will be standardized at 150% of body weight loss during exercise-induced dehydration to ensure consistency across trials.

See the study design expressed as a figure below:

Measurements:

All measurements will be performed at one of the test rooms at our lab facility that boarders the room where participants can relax and wait out the end of the study day. When certain measurements need to be performed they simply move from one room to the next. Duration of taking blood and urine samples will take not more than 30-90 seconds each and perceptual data will take 30-60 seconds to be reported. To allow to perform all measurements, participants will remain supervised by a team member in the lab for up to 7 hours per trial. Therefore, there will be a separate space available that allows for self-study or watching TV that includes a chair and table to work on and a couch to relax.

Blood samples (7 per trial):

Blood samples will be taken via a venous catheter attached to an extension tube and a stopcock. After each blood sample sterile saline solution will be infused in the extention tube to avoid clotting. The iv catheter will be placed by one of the Clinical Research Center phlebotomists. A total of seven 10 milliliters blood samples will be collected on the euhydrated baseline, dehydrated baseline and at 30, 60, 120, 180, and 240 minutes of the rehydration period.

All samples will be analyzed for glucose, osmolality, total plasma protein, hematocrit, hemoglobin (for plasma volume changes), sodium, potassium, and chloride.

Urine samples (6 per trial):

Urine samples will be provided from the subjects in a urine cup. The cup will be placed in the pass-through window where a lab technician will receive it and process it.

Urine samples will be collected using ~900 milliliters cups at euhydrated baseline, dehydrated baseline and cumulative totals will be collected at 60, 120, 180, and 240 minutes of the rehydration period. All samples will be analyzed for urine osmolality, specific gravity, volume, sodium, and potassium.

Sweat samples (1 per trial):

Sweat samples will be collected with sweat patches. The sweat patches are like a band-aid that it will be placed on the forearm and stay there for approximately 30 min to absorb sweat. After 30 minutes the patch will be removed and the sweat sample will be extracted by centrifugation in the lab.

Sweat samples will be collected during the first 30 min of exercise to assess sodium and potassium content.

Skin Hydration (6 per trial):

Skin hydration will be measured with a pen-like instrument that applies a very light pressure on the skin. This does not cause any pain or skin irritation.

Skin hydration will be measured on the face (inner zygomatic bone), arm (bicep brachii) at euhydrated baseline, 30 minutes after the end of dehydration, and at 60, 120, 180, and 240 minutes of rehydration.

Gastrointestinal Symptoms (6 per trial):

Gastrointestinal symptoms will be assessed at 1, 2, 3 and 4 hours of rehydration via Gastrointestinal Symptom Rating Scale (GSRS).

Perceptual Data (6 per trial, maximal 9 minutes):

Thirst, stomach fullness, and taste, assessed via a visual analog scale.

• Study Type: Interventional
• Enrollment (Actual): 15
• Phase: Not Applicable

Contacts and Locations

Study Locations

• United States
  • Arizona
    • Phoenix, Arizona, United States, 85004
      • Hydration Science Lab

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: Adult
• Accepts Healthy Volunteers: Yes

Description

Inclusion Criteria:

• Recreationally physically active: Training > 2x per week and ≤ 6hours per week
• BMI of 18.5-29.9
• Stable weight for the last 2 months (<5 pounds. fluctuation)

Exclusion Criteria:

• Women who are pregnant, breast feeding, or planning to become pregnant during the trial
• night shifting work
• thyroid medication
• bariatric surgery
• cardiovascular disease
• renal disease
• hepatic disease
• Participating in another study at the same time
• Bodyweight <110 pounds
• Currently Smoker
• Major surgery, including abdominal, in the past 3 months or individuals who have planned surgery during the trial.
• Alcohol or drug abuse within the last 12 months
• High alcohol intake (average of > 2 standard drinks per day or > 10 standard drinks per week)

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: Single

Number of Arms

3

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: ConcenTrace; Beverage with ConcenTrace minerals	Dietary supplement: ConcenTrace; 1.25 milliliter of concentrace per liter
Experimental: ZeroLyte; Beverage with high level of trace elements but no sugar	Dietary supplement: ZeroLyte; Beverage with 1 pack of ZeroLyte per 500 milliliter of water
Placebo Comparator: Placebo; Water with non caloric, non-sodium containing sweetner	Dietary supplement: Placebo; Non- calorically sweetened Water

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Net Fluid Balance	Body water Retention after exercise-induced dehydration	at 60 minutes, 120 minutes, 180 minutes and 240 minutes of rehydration
Urine volume	Urine volume during the rehydration period	1, 2, 3, and 4 hour of the rehydration period
Free water clearance	Free water clearance during the rehydration period	1, 2, 3, and 4 hours of the rehydration period
Osmotic Clearance	Osmotic renal clearance during the rehydration period	1, 2, 3, and 4 hours of the rehydration period
Skin Hydration	Skin hydration at the face assessed with a moisture meter.	1, 2, 3, and 4 hours of the rehydration period

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Plasma Volume	Changes in Plasma Volume during rehydration based on hematocrit and hemoglobin	1, 2, 3, and 4 hours of the rehydration period
Stomach fullness	Stomach fullness assessed during dehydration	1, 2, 3, and 4 hours of the rehydration period
Gastrointestinal symptoms	Gastrointestinal symptoms assessed with the structured assessment of gastrointestinal symptoms scale	At the end of the 4 hours rehydration period
Plasma Osmolality	Plasma osmolality during rehydration	1, 2, 3, and 4 hours of the rehydration period

Collaborators and Investigators

• Sponsor: Arizona State University

Study record dates

Study Major Dates

• Study Start (Actual): March 26, 2025
• Primary Completion (Actual): August 29, 2025
• Study Completion (Actual): August 29, 2025

Study Registration Dates

• First Submitted: February 28, 2025
• First Submitted That Met QC Criteria: February 28, 2025
• First Posted (Actual): March 5, 2025

Study Record Updates

• Last Update Posted (Estimated): September 5, 2025
• Last Update Submitted That Met QC Criteria: September 3, 2025
• Last Verified: September 1, 2025

More Information

Terms related to this study

• Keywords: fluid balance; rehydration; water retention; sports drinks
• Additional Relevant MeSH Terms: Pathologic Processes; Metabolic Diseases; Water-Electrolyte Imbalance; Pathological Conditions, Signs and Symptoms; Nutritional and Metabolic Diseases; Dehydration; Substandard Drugs; Pharmaceutical Preparations; Counterfeit Drugs
• Other Study ID Numbers: STUDY00021498

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: UNDECIDED

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No
• product manufactured in and exported from the U.S.: No