Examination of the Effect of Nd:YAG Laser on Oxidative Stress and Matrix Metalloproteinase-9 in Teeth With Apical Periodontitis

December 7, 2025 updated by: Ali Turkyilmaz, Kırıkkale University
The objective of this clinical investigation is to examine the impact of Nd:YAG laser therapy on oxidative stress and matrix metalloproteinase-9 (MMP-9) levels in teeth afflicted with apical periodontitis. Samples were collected from patients who had undergone treatment with the Nd:YAG laser and compared with samples collected from patients who had not received this treatment. The study commenced with a sample of 60 patients. However, the study was completed with 58 patients. The study concluded with the following finding: the use of Nd:YAG lasers was not found to be superior.

Study Overview

Status

Completed

Conditions

Study Type

Observational

Enrollment (Actual)

58

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Turkey (Türkiye)
      • Kırıkkale, Turkey (Türkiye)
        • Kırıkkale Üniversitesi Diş Hekimliği Fakültesi

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

No

Sampling Method

Probability Sample

Study Population

The study groups comprised of patients who had applied to the Department of Endodontics at the Faculty of Dentistry at Kırıkkale University and that underwent endodontic treatment from 15 February 2024 to 15 October 2024.

Description

Inclusion Criteria:

  • Patients aged between 25 and 50 years and classified as American Society of Anesthesiologists (ASA) I. The maxillar and mandibular incisors and premolars with chronic apical periodontitis of a PAI score of 3 or 4 were included.

Exclusion Criteria:

  • Patients with American Society of Anesthesiologists (ASA) classification II or higher, pregnant, patients with localised or generalised periodontitis, patients who had used antibiotics in the last month and anti-inflammatory drugs in the last week, smokers, and teeth with internal or external root resorption, swelling, palpation pain or fistula in the relevant area, root fracture or incomplete root formation, and teeth that could not be isolated with rubber dam were not included in the study.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

Cohorts and Interventions

Group / Cohort
Nd:YAG laser group
Group treated with Nd:YAG laser
Control group
Group not treated with Nd:YAG laser

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Measurement of TOS, TAOC and MMP-9 levels in periapical exudate and GCF
Time Frame: The initial and seventh days of the treatment period
TOS, TAOC and MMP-9 levels were measured by ELISA in periapical exudate and GCF samples collected during routine root canal treatment in patients with apical periodontitis. No significant difference was detected in the change in biomarker levels between the group treated with the Nd:YAG laser and the control group.
The initial and seventh days of the treatment period

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Determination of the correlation relationship between TOS, TAOC and MMP-9 levels in periapical exudate and GCF samples
Time Frame: The initial and seventh days of the treatment period
The present study investigated the correlation between periapical exudate and GCF samples. The findings revealed a unidirectional and strong relationship between TOS and TAOC.
The initial and seventh days of the treatment period

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Kırıkkale University

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

February 1, 2024

Primary Completion (Actual)

November 1, 2024

Study Completion (Actual)

November 8, 2024

Study Registration Dates

First Submitted

November 17, 2025

First Submitted That Met QC Criteria

November 17, 2025

First Posted (Estimated)

November 21, 2025

Study Record Updates

Last Update Posted (Actual)

December 15, 2025

Last Update Submitted That Met QC Criteria

December 7, 2025

Last Verified

November 1, 2025

More Information

Terms related to this study

Keywords

Additional Relevant MeSH Terms

Other Study ID Numbers

  • 2024/046

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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