Carbonic Anhydrase II Responses to Basketball-Specific High-Intensity Interval Training (CAII-BASKET)

January 28, 2026 updated by: Recep Soslu, Karamanoğlu Mehmetbey University

Erythrocyte Carbonic Anhydrase II as a Marker of Metabolic Adaptation to Basketball-Specific High-Intensity Interval Training

This study aims to examine whether erythrocyte carbonic anhydrase II can be used as a biological marker of metabolic adaptation to basketball-specific high-intensity interval training. Carbonic anhydrase II is an enzyme involved in acid-base balance and carbon dioxide transport, and it may reflect physiological adaptations to intense exercise.

Healthy basketball players participated in a structured high-intensity interval training program designed to reflect the physical demands of basketball. Blood samples and performance-related measurements were collected before and after the training period to evaluate changes in erythrocyte carbonic anhydrase II levels and related metabolic responses.

The findings of this study are expected to improve the understanding of exercise-induced metabolic adaptations and to explore the potential use of erythrocyte carbonic anhydrase II as a practical biomarker for monitoring training responses in basketball players.

Study Overview

Status

Completed

Conditions

Intervention / Treatment

Detailed Description

This study was designed to investigate erythrocyte carbonic anhydrase II as a potential biomarker of metabolic adaptation to basketball-specific high-intensity interval training. Carbonic anhydrase II plays a key role in acid-base regulation and carbon dioxide transport, which are critical physiological processes during repeated high-intensity exercise.

Participants were healthy basketball players who completed a structured high-intensity interval training program reflecting the intermittent and high-demand nature of basketball activity. The training protocol consisted of repeated bouts of intense exercise interspersed with short recovery periods, consistent with established basketball-specific conditioning practices.

Blood samples were obtained at predefined time points to assess erythrocyte carbonic anhydrase II levels. These biochemical measurements were evaluated alongside selected performance and physiological indicators to characterize training-induced metabolic adaptations. All assessments were conducted under standardized conditions to minimize variability related to diet, hydration, and recent physical activity.

The study followed an observational pre-post design without clinical intervention beyond the prescribed training program. No experimental drugs or medical devices were used. Safety monitoring was conducted throughout the study period, and no serious adverse events were anticipated given the low-risk nature of the exercise protocol and the healthy status of the participants.

This study seeks to contribute to the growing body of research on exercise-related biomarkers by examining whether erythrocyte carbonic anhydrase II may provide practical insight into metabolic adaptation to sport-specific high-intensity training.

Study Type

Interventional

Enrollment (Actual)

24

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Turkey (Türkiye)
    • Karaman
      • Karaman, Karaman, Turkey (Türkiye), 70200
        • Karamanoğlu Mehmetbey University, Faculty of Sport Sciences

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

  • Adult

Accepts Healthy Volunteers

Yes

Description

Inclusion Criteria:

Healthy male and female basketball players aged 18 years or older

Actively participating in regular basketball training at the time of the study

Free from known cardiovascular, metabolic, or respiratory diseases

No musculoskeletal injury that could limit participation in high-intensity exercise

Willingness to participate in a basketball-specific high-intensity interval training program

Ability to provide written informed consent

Exclusion Criteria:

Presence of any diagnosed cardiovascular, metabolic, or respiratory disease

Use of medications or supplements known to affect metabolic or acid-base balance

Acute or chronic musculoskeletal injury within the previous three months

Participation in another structured training or research intervention during the study period

Failure to comply with the training protocol or assessment procedures

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Basic Science
  • Allocation: N/A
  • Interventional Model: Single Group Assignment
  • Masking: None (Open Label)

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Experimental: Basketball-Specific High-Intensity Interval Training
Participants completed a basketball-specific high-intensity interval training program designed to induce metabolic adaptations. All participants were assigned to this single intervention arm and completed the same training protocol.
Behavioral: Basketball-Specific High-Intensity Interval Training
A structured basketball-specific high-intensity interval training program consisting of repeated bouts of intense exercise interspersed with short recovery periods, designed to induce metabolic adaptations.

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in Erythrocyte Carbonic Anhydrase II Levels
Time Frame: Baseline( week 0) and post training (week 8)
The primary outcome is the change in erythrocyte carbonic anhydrase II levels measured before and after the basketball-specific high-intensity interval training program. Erythrocyte carbonic anhydrase II was assessed from blood samples as an indicator of metabolic adaptation to high-intensity exercise.
Baseline( week 0) and post training (week 8)

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Karamanoğlu Mehmetbey University

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Actual)

November 1, 2025

Primary Completion (Actual)

January 10, 2026

Study Completion (Actual)

January 15, 2026

Study Registration Dates

First Submitted

January 22, 2026

First Submitted That Met QC Criteria

January 28, 2026

First Posted (Actual)

January 30, 2026

Study Record Updates

Last Update Posted (Actual)

January 30, 2026

Last Update Submitted That Met QC Criteria

January 28, 2026

Last Verified

January 1, 2026

More Information

Terms related to this study

Keywords

Other Study ID Numbers

  • 03-2023/02

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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