Tregs and miRNAs in Breast Milk: Effects on Infant Food Tolerance
Immunoregulatory Factors in Human Breast Milk: Relationship Between Regulatory T Cells (Tregs), miRNAs and Food Tolerance
調査の概要
状態
詳細な説明
Breast milk contains microRNAs (miRNAs) that contribute to the maturation of the infant immune system by influencing immune cell development, inflammatory mediator secretion, and maintenance of immunological homeostasis. Certain miRNAs, such as miR-92 and miR-17, play a role in B- and T-cell maturation, while miRNA-10a is associated with regulatory T cells (Tregs), which can modulate food allergic responses. This study aims to identify immunoregulatory Treg-associated miRNAs in breast milk, particularly those involved in the development and function of Treg cells; to analyze their expression in colostrum, transitional milk, and mature breast milk; to investigate their effects on Treg cells; and to examine the relationship between food tolerance and miRNA-Treg interactions.
Laboratory analyses will be conducted on maternal breast milk and infant blood samples. Breast milk samples will be manually expressed between 09:00 and 14:00 into sterile collection containers, with 7-10 mL collected per session. Five milliliters of each sample will be transferred into Eppendorf tubes and transported on ice within 30 minutes for molecular analysis. Each sample will be aliquoted into sterile 2 mL cryotubes, flash-frozen, and stored at -80°C until RNA isolation. The remaining 2-5 mL of each breast milk sample will be transported on ice within 30 minutes for immunologic and cytometric analyses. Infant blood samples obtained during routine follow-up visits will be analyzed for Treg-associated miRNAs and their target gene expression to evaluate correlations with maternal breast milk miRNA profiles and infant immune tolerance.
All laboratory analyses will be conducted using standard equipment and procedures in certified Genetics and Immunology laboratories. Through this approach, the study will examine longitudinal changes in Treg-associated miRNAs in breast milk, their correlation with infant immune parameters, and their potential role in promoting immune tolerance and preventing food allergy development. The results are expected to provide insights into early-life immune programming and may inform novel therapeutic interventions and preventive strategies.
研究の種類
入学 (推定)
連絡先と場所
研究連絡先
- 名前：Gonca Keskindemirci, MD, PhD
- 電話番号：0905324934651
- メール：keskindemirci@hotmail.com
研究場所
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Istanbul、トルコ（Türkiye）
- 募集
- Child Health and Diseases Clinic, Esencan Hospital
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コンタクト：
- Özden Durmuş Gönültaş, MD
- 電話番号：905073121218
- メール：ozdengnlts@gmail.com
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副調査官：
- Özden Durmuş Gönültaş, MD
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Istanbul、トルコ（Türkiye）
- 募集
- İstanbul University İstanbul Medical Faculty Department of Pediatrics Division of Social Pediatrics
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コンタクト：
- Gonca Keskindemirci, MD, PhD
- 電話番号：0905324934651
- メール：keskindemirci@hotmail.com
-
副調査官：
- Özden Durmuş Gönültaş, MD
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主任研究者：
- Gonca Keskindemirci, Prof
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主任研究者：
- Gülbin E Gökçay, Prof
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主任研究者：
- Günnur Deniz, Prof
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主任研究者：
- Asuman Gedikbaşı, Prof
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主任研究者：
- Birsen Karaman, Prof
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主任研究者：
- Bilge Ş Selçuk, Assoc Prof
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副調査官：
- Gizem Kara Elitok, Assoc Prof
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副調査官：
- Ebru Kılıç
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副調査官：
- Ayşenur Kökoğlu
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副調査官：
- Suzin Tatonyan
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参加基準
適格基準
就学可能な年齢
- 子
健康ボランティアの受け入れ
サンプリング方法
調査対象母集団
説明
Inclusion Criteria:
- Lactating mothers and their infants attending the Child Health and Diseases Clinic at Istanbul University Faculty of Medicine, or the Child Health and Diseases Clinic at Esencan Hospital.
- Infants who are exclusively breastfed for the first 6 months.
- Mothers willing to provide breast milk samples at multiple time points (colostrum, transitional milk, and mature milk at 1, 4, 6, and/or 9 months postpartum).
- Infants aged 0-9 months, born at term (≥37 weeks gestation) with no major congenital anomalies or chronic illnesses.
- Mothers and infants who provide informed consent for participation in the study.
Exclusion Criteria:
- Infants who are not exclusively breastfed for the first 6 months.
- Infants with chronic illnesses or diagnosed immunodeficiency, metabolic, or gastrointestinal disorders.
- Mothers who are unable or unwilling to provide informed consent.
- Mothers with chronic illnesses or immunological disorders that may affect breast milk composition.
研究計画
研究はどのように設計されていますか？
デザインの詳細
この研究は何を測定していますか？
主要な結果の測定
結果測定 |
メジャーの説明 |
時間枠 |
|---|---|---|
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Expression Levels of Treg-Associated miRNAs in Breast Milk
時間枠：Colostrum (day 0-7), days 10-15 (transitional milk), and at 1, 4, 6, and 9 months postpartum
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Quantitative analysis of regulatory T cell (Treg)-associated microRNA (miRNA) expression levels in breast milk samples.
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Colostrum (day 0-7), days 10-15 (transitional milk), and at 1, 4, 6, and 9 months postpartum
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協力者と研究者
スポンサー
研究記録日
主要日程の研究
研究開始 (実際)
一次修了 (推定)
研究の完了 (推定)
試験登録日
最初に提出
QC基準を満たした最初の提出物
最初の投稿 (実際)
学習記録の更新
投稿された最後の更新 (実際)
QC基準を満たした最後の更新が送信されました
最終確認日
詳しくは
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