Evaluation of Fluid Biomarkers Around Implants Exhibiting Early Stage Periimplant Bone Loss
7. mai 2026 oppdatert av: Raziye Tugce Can Keles, Pamukkale University
Evaluation of Biomarkers Involved In Bone Metabolism (Remodeling) In Periimplanter Crevicular Fluid of Implants With Early Marginal Bone Loss: A Retrospective Clinical Study
This study aimed to compare biological mediators known to be important in peri-implant diseases in implants with and without early stage marginal bone loss.
A total of 118 implants in 59 patients were designated as test and control groups.
The radiographic and periodontal data of the included implants, along with peri-implant sulcus fluid samples, were evaluated.
Studieoversikt
Status
Fullført
Forhold
Intervensjon / Behandling
Detaljert beskrivelse
This study included clinical and radiographic records of patients who received implant treatment in the maxilla and/or mandible between 11/2021 and 9/2024.
Patients were divided into test and control groups based on the presence/absence of EMBL after prosthetic loading.
Periodontal clinical measurements were recorded and peri-implant sulcus fluid (PISF) samples were collected.
Sclerostin, calprotectin, type I collagen cross-linked N-telopeptide (NTx), matrix metalloproteinase (MMP)-8, and MMP-9 levels were analyzed using ELISA.
Group comparisons were performed with the statistical analyses.
Studietype
Observasjonsmessig
Registrering (Faktiske)
59
Kontakter og plasseringer
Denne delen inneholder kontaktinformasjon for de som utfører studien, og informasjon om hvor denne studien blir utført.
Studiesteder
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Denizli, Tyrkia (Türkiye), 20160
- Pamukkale University, Faculty of Dentistry, Periodontology
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Deltakelseskriterier
Forskere ser etter personer som passer til en bestemt beskrivelse, kalt kvalifikasjonskriterier. Noen eksempler på disse kriteriene er en persons generelle helsetilstand eller tidligere behandlinger.
Kvalifikasjonskriterier
Alder som er kvalifisert for studier
- Barn
- Voksen
- Eldre voksen
Tar imot friske frivillige
Ja
Prøvetakingsmetode
Ikke-sannsynlighetsprøve
Studiepopulasjon
Patients who received implant treatment at the Department of Periodontology, Pamukkale University Faculty of Dentistry.
Beskrivelse
Inclusion Criteria:
- Presence of 1 mm or more of radiographic marginal bone loss within the first 3 months after implant placement
- Availability of radiographic documentation
- Availability of demographic data, surgical records, and data related to the implant used
- Physical condition classified as Class I and II according to the American Society of Anesthesiologists (ASA) classification
- Patients with no smoking history in the last 10 years
- Fixed implant-supported restorations
- Screw-retained implants
Exclusion Criteria:
- Patients receiving and/or maintaining implant treatment at centers other than the Department of Periodontology, Pamukkale University Faculty of Dentistry
- Patients with incomplete or unreachable files or radiographic documentation
- Patients with a known systemic disease/condition that alters their physical condition or bone metabolism
- Severe bruxism cases
- Pregnant and breastfeeding patients
- Patients with incomplete implant-supported prosthesis
- Areas grafted during or before implant surgery
- Removable implant-supported restorations
- Cement-retained implants
- Patients undergoing peri-implantitis treatment
Studieplan
Denne delen gir detaljer om studieplanen, inkludert hvordan studien er utformet og hva studien måler.
Hvordan er studiet utformet?
Designdetaljer
Kohorter og intervensjoner
Gruppe / Kohort |
Intervensjon / Behandling |
|---|---|
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Test
Presence of marginal bone loss of 1 mm or more within the first 3 months after implant placement
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To evaluate biomarkers associated with peri-implant bone loss, periopaper was placed in the peri-implant pocket and absorbed with sulcus fluid.
Parallel cone technique was used during periapical imaging to accurately measure peri-implant bone loss.
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Control
Presence of less than 1 mm of marginal bone loss within the first 3 months after implant placement
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To evaluate biomarkers associated with peri-implant bone loss, periopaper was placed in the peri-implant pocket and absorbed with sulcus fluid.
Parallel cone technique was used during periapical imaging to accurately measure peri-implant bone loss.
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Hva måler studien?
Primære resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
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Calprotectin levels in peri-implant crevicular fluid
Tidsramme: 6-8 week after prosthetic treatment
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After collecting peri-implant sulcus fluid using the paper strip method, calprotectin levels in the peri-implant sulcus region were determined using the ELISA method and recorded in ng/ml.
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6-8 week after prosthetic treatment
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Sclerostin levels in peri-implant crevicular fluid
Tidsramme: 6-8 week after prosthetic treatment
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After collecting peri-implant sulcus fluid using the paper strip method, sclerostin levels in the peri-implant sulcus region were determined using the ELISA method and recorded in pg/ml.
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6-8 week after prosthetic treatment
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NTx levels in peri-implant crevicular fluid
Tidsramme: 6-8 week after prosthetic treatment
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After collecting peri-implant sulcus fluid using the paper strip method, NTx levels in the peri-implant sulcus region were determined using the ELISA method and recorded in ng/ml.
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6-8 week after prosthetic treatment
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MMP-8 levels in peri-implant crevicular fluid
Tidsramme: 6-8 week after prosthetic treatment
|
After collecting peri-implant sulcus fluid using the paper strip method, MMP-8 levels in the peri-implant sulcus region were determined using the ELISA method and recorded in ng/ml.
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6-8 week after prosthetic treatment
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MMP-9 levels in peri-implant crevicular fluid
Tidsramme: 6-8 week after prosthetic treatment
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After collecting peri-implant sulcus fluid using the paper strip method, MMP-9 levels in the peri-implant sulcus region were determined using the ELISA method and recorded in ng/ml.
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6-8 week after prosthetic treatment
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Andre resultatmål
Resultatmål |
Tiltaksbeskrivelse |
Tidsramme |
|---|---|---|
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Radiographic detecting of Peri-implant marginal bone loss
Tidsramme: Baseline, 3 months
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The amount of marginal bone loss was determined by comparing the periapical radiograph taken on the day the implants were placed with the radiograph taken at 3 months.
Measurements of digital radiographic images were performed using computer software.
Then, study groups were determined according to the presence or absence of peri-implant marginal bone loss.
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Baseline, 3 months
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Modified Bleeding İndex
Tidsramme: During the same session as the peri-implant fluid collection procedure
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This assessment was performed based on the bleeding response following gentle probing with a plastic probe in the four regions of each implant (mesial, distal, palatal/lingual, and buccal).
The severity of bleeding observed within approximately 10-30 seconds after probing was scored and recorded.
The scoring system was graded as follows: 0: no bleeding, 1: bleeding at a single point, 2: bleeding at several points or in a linear pattern, 3: heavy bleeding following probing.
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During the same session as the peri-implant fluid collection procedure
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Peri-implant pocket depth
Tidsramme: During the same session as the peri-implant fluid collection procedure
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A plastic periodontal probe was used to determine peri-implant pocket depth.
Measurements were taken by using the probe, supported by its own weight, parallel to the long axis of the tooth.
Pocket depth was measured at 6 sites (mesio-buccal, buccal, disto-buccal, mesio-palatal/lingual, palatal/lingual, disto-palatal/lingual) for each implant included in the study and recorded in millimeters (mm).
The pocket depth value for each dental implant was obtained by calculating the average of the 6 measured values.
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During the same session as the peri-implant fluid collection procedure
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Gingival İndex
Tidsramme: During the same session as the peri-implant fluid collection procedure
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The mesial, distal, buccal, and lingual/palatal surfaces of each tooth/implant were evaluated separately, and the average of the scores obtained was recorded as the gingival index value for that tooth/implant.
Scoring during the evaluation was performed as follows: 0 = normal gingiva, 1 = mild inflammation (color change, mild edema, no bleeding on probing), 2 = moderate inflammation (redness, edema, bleeding on probing), 3 = severe inflammation (marked redness, edema, ulceration, spontaneous bleeding).
Subsequently, all values were summed and divided by the total number of teeth and implants to record the gingival index score.
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During the same session as the peri-implant fluid collection procedure
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Modified Plaque İndex
Tidsramme: During the same session as the peri-implant fluid collection procedure
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The mesial, distal, buccal, and lingual/palatal surfaces around the teeth/implants were examined, and the average of the scores obtained for each surface was recorded as the plaque index for that tooth/implant.
The scoring criteria were as follows: 0 = no plaque, 1 = plaque detectable only with a probe, 2 = soft plaque accumulation visible to the naked eye, 3 = the tooth surface covered with a distinct plaque layer.
Subsequently, all values were summed and divided by the total number of teeth and implants to record the modified plaque index score.
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During the same session as the peri-implant fluid collection procedure
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Peri-implant mucosal recession
Tidsramme: During the same session as the peri-implant fluid collection procedure
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The abutment-implant interface was designated as the reference point for determining the extent of peri-implant mucosal recession, and the distance between the reference point and the free gingival margin was measured using a periodontal probe.
Mucosal recession was recorded in four regions (mesio-buccal, mid-buccal, disto-buccal, mid-lingual/palatal) for each implant included in the study.
The recession value for each implant was obtained by calculating the average of the four measured values.
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During the same session as the peri-implant fluid collection procedure
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Samarbeidspartnere og etterforskere
Det er her du vil finne personer og organisasjoner som er involvert i denne studien.
Sponsor
Samarbeidspartnere
Etterforskere
- Hovedetterforsker: Raziye Tugce Can Keles, R.A., Pamukkale University Faculty of Dentistry, Department of Periodontology
- Studieleder: Gizem Torumtay Cin, Assoc. Prof., Pamukkale University Faculty of Dentistry, Department of Periodontology
Studierekorddatoer
Disse datoene sporer fremdriften for innsending av studieposter og sammendragsresultater til ClinicalTrials.gov. Studieposter og rapporterte resultater gjennomgås av National Library of Medicine (NLM) for å sikre at de oppfyller spesifikke kvalitetskontrollstandarder før de legges ut på det offentlige nettstedet.
Studer hoveddatoer
Studiestart (Faktiske)
6. mars 2025
Primær fullføring (Faktiske)
6. september 2025
Studiet fullført (Faktiske)
6. september 2025
Datoer for studieregistrering
Først innsendt
3. mai 2026
Først innsendt som oppfylte QC-kriteriene
7. mai 2026
Først lagt ut (Faktiske)
13. mai 2026
Oppdateringer av studieposter
Sist oppdatering lagt ut (Faktiske)
13. mai 2026
Siste oppdatering sendt inn som oppfylte QC-kriteriene
7. mai 2026
Sist bekreftet
1. mai 2026
Mer informasjon
Begreper knyttet til denne studien
Nøkkelord
Ytterligere relevante MeSH-vilkår
Andre studie-ID-numre
- E-60116787-020-604271
- 2025DİŞF001 (Annen identifikator: Office of Scientific Research Projects of Pamukkale University)
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