SIT LESS 3: The Effect of Low Intensity Physical Activity on Insulin Sensitivity, Mood and Cognitive Performance (SIT LESS 3)

Inclusion Criteria:

• Signed informed consent
• Men and postmenopausal women: 40-80 years old
• BMI: 25.0 - 35.0 kg/m2
• Maximum 2.5 hours of MVPA per week (during last 3 months)
• Having a general practitioner
• Agreeing to be informed about medically relevant personal test-results by a physician
• Accessible veins on arms as determined by examination at screening

Exclusion Criteria:

• Reported participation in another biomedical trial which may have an effect on insulin sensitivity one month before the pre-study examination or during the study
• Blood donation in the past three months
• Reported participation in night shift work 2 weeks prior to pre-study investigation or during the study. Night work is defined as working between midnight and 6.00 AM.
• Consumption of >14 (women) or > 21 (men) alcoholic units per week
• Reported dietary habits: medically prescribed diet, slimming diet;
• Reported weight loss (>2kg) in the last three months prior to the screening;
• Not being able to execute at least three (out of four) cognition tests in the training session
• Not being able to execute the sit less try-out day
• Being an employee of Unilever or the collaborating research departments in Maastricht University Medical Centre
• Experimental drug use (during the last 3 months)
• Use of insulin, oral blood glucose lowering medication (metformin and/or SU-derivatives and/or DPP-IV inhibitors), corticosteroids or vitamin K antagonists in the last 3 months
• Fasting plasma glucose level > 6.9 mmol/L
• Medical conditions which make participation in the study not responsible which will be decided by a medical doctor during screening
• Other clinically relevant abnormalities in clinical chemistry at screening (to be judged by a medical doctor)
• Mental or physical disability which interferes with physical activity