Sodium Channel Expression in Human Teeth
研究概览
地位
条件
详细说明
The human tooth pulp is a rich source of pain fibers and is a common site of pathology that is often accompanied by spontaneous and stimulus-induced lingering pain. A common treatment modality includes the extraction of the offending tooth diagnosed with irreversible pulpitis. Extracted teeth represent an abundant source of normal and diseased human nociceptors and the evaluation of these tissues represents a powerful model to study human pain mechanisms since the character of pain, pain levels, and response to stimuli can be documented prior to extraction.
The overall objective of this study is to correlate changes in the expression of Sodium Channels (NaCh) with clinical responses to hot and cold thermal stimuli, and the expression of the associated receptors/transducers responsible for receiving that stimulus in extracted teeth with severe and spontaneous pain. Teeth requiring extraction in the clinical setting will be used for this study.
The research questions are: 1.) To evaluate quantitatively the overall NaCh and Nav 1.3, 1.6, 1.7, 1.8 1.9 isoform expressions in nerves of normal teeth as compared to diseased teeth 2.) To evaluate quantitatively hot/cold VR1 and CMR1 receptor expression in nerves of normal teeth as compared to the nerves in the modality-specific pain groups of diseased teeth 3.) To investigate the ultrastructural localization of NaCh isoforms in different fiber types and at sites that may be involved in pain generation.
研究类型
注册 (实际的)
联系人和位置
学习地点
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Texas
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San Antonio、Texas、美国、78229
- University of Texas Health Science Center, San Antonio Dental School
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参与标准
资格标准
适合学习的年龄
接受健康志愿者
有资格学习的性别
取样方法
研究人群
描述
Inclusion Criteria:
- individuals 16-80 years old
- normal third molar(wisdom)teeth
- painful, diseased teeth requiring extraction
Exclusion Criteria:
- individuals under age 16 or above age 80
- pregnant females
- prisoners
- nonvital painful teeth
学习计划
研究是如何设计的?
设计细节
- 观测模型:病例对照
- 时间观点:横截面
队列和干预
团体/队列 |
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Normal Teeth
Normal molar teeth
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painful teeth
Painful molar teeth
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研究衡量的是什么?
主要结果指标
结果测量 |
措施说明 |
大体时间 |
---|---|---|
Sodium Channel (NaCh) expression in nerves of normal teeth compared to diseased teeth.
大体时间:Immediately following extration of the tooth.
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Ex vivo lab bench study utilizing extracted human teeth.
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Immediately following extration of the tooth.
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次要结果测量
结果测量 |
措施说明 |
大体时间 |
---|---|---|
Vanilloid receptor subtype-1 (VR1) and cold-and menthol-sensitive receptor-1(CMR1) expression in normal vs diseased teeth.
大体时间:Immediately following extraction of tooth.
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Ex vivo bench lab study utilizing extracted human teeth.
|
Immediately following extraction of tooth.
|
合作者和调查者
调查人员
- 首席研究员:Michael A. Henry, DDS, PhD、University of Texas Health Science Center at San Antonio, TX
研究记录日期
研究主要日期
学习开始
初级完成 (实际的)
研究完成 (实际的)
研究注册日期
首次提交
首先提交符合 QC 标准的
首次发布 (估计)
研究记录更新
最后更新发布 (估计)
上次提交的符合 QC 标准的更新
最后验证
更多信息
与本研究相关的术语
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