Physical Fitness Effect on the Different Oxidative Stress Measurements

Assessing Physical Fitness Effect on the Different Oxidative Stress Measurements, at Rest and After Acute Exercise

Physical exercise has many benefits, although it might also have negative impact on the body, which changes in accordance with training level, length of workout, age of subject and their fitness level. These outcomes are governed by complex biological processes, from which Reactive Oxygen Substances (ROS) are considered the most prominent ones during exercise activity. These substances harm muscle activity, and might cause other health related damage by influencing other pathways that engage with free radicals. Excess of ROS, is expressed as Oxidative Stress (OS), pathogenic process activation and aging. With this in mind, it is important to remember that ROS are essential to the investigators bodies as well, especially in gene regulation. Thus they influence proteins that are important for proper muscle function and the immune system.

Physical exercise induces changes in low molecular weight antioxidants, and in antioxidant enzymes. According to results that were published in the literature, no universal conclusion can be made regarding physical exercise effect on antioxidants activity. These contradictions might be attributed to the difference between the methods that were used, and their quality.

Paradoxically, physical exercise has the same effect on ROS inducement in skeletal muscle fibers as does not using the muscles for a long period of time (especially as a result of handicap). It is well known that physical exercise effect oxidative stress and antioxidant. Due to the doubts that rise from the literature, the investigators think that it is important to test physical fitness effect on oxidative stress biological markers, using various high quality methods, on the same population.

The investigators will test healthy men, 20-35 years of age, none smokers with 6-24% fat, who do not take vitamins or supplements. The men will run on a treadmill for 10 minutes to exhaustion, and blood will be taken before the test, 5 minutes after the test, and an hour after the test (checking recovery).

Study Overview

• Status: Completed
• Conditions: Oxidative Stress
• Intervention / Treatment: Other: Stress test

Detailed Description

Things the investigators will measure:

1. Filling out a physical fitness, and overall health form, that indicates all fitness activity in the past 3 years, and vitamins and supplements that are being taken.
2. Fat percentage
3. VO2 max
4. Oxidants and antioxidants activity: Super oxide dismutase, Catalase, GSH/GSSG, Glutathione peroxide, Glutathione reductase, LDL, conjugated dienes, Plasma malondialdehyde. Measurements will be taken before the test, 5 minutes after the test and an hour after the test.

Work plan:

50 men, at the ages of 20-35 will be recruited. The participants will fill out a form that indicates their physical activity in the past 3 years, as well as their vitamin and supplements intake.

The men will run on a treadmill for 10 minutes until exhaustion.

Blood will be drawn before, 5 minutes after and an hour after the test. Oxidative stress will be measured according to the following parameters:

• 10 ml of blood in citrate tube will be centrifuged and analyzed for: Super oxide dismutase, Catalase, GSH/GSSG, Glutathione peroxide, Glutathione reductase.
• 2 ml of blood with no coagulating factors will be centrifuged and analyzed for: LDL, conjugated dienes, Plasma malondialdehyde (TBARS).

Clinical research:

Participants will run on a treadmill for 10 minutes until exhaustion. The men will be divided into 5 groups according to their VO2 max, comparing the oxidants and antioxidants activity between the groups.

Checking the oxidative stress and VO2 dependency (VO2 is an index for physical fitness).

Age: 20-35 Sex: Men Inclusion criteria: Healthy men, 2-35 years of age, with 6-24% body fat. Exclusion criteria: Smokers, and people who use vitamins and supplements.

• Study Type: Interventional
• Enrollment (Actual): 32
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Israel
  • Netanya, Israel, 42902
    • Wingate Institute

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 20 years to 35 years (ADULT)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• Healthy men
• 20-35 years of age, with 6-24% body fat.

Exclusion Criteria:

• Smokers and subjects who use vitamins and supplements

Study Plan

How is the study designed?

Design Details

• Primary Purpose: BASIC_SCIENCE
• Allocation: NA
• Interventional Model: SINGLE_GROUP
• Masking: NONE

Number of Arms

1

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
EXPERIMENTAL: Stress test; The men will run on a treadmill for 10 minutes until exhaustion. Blood will be drawn before, 5 minutes after and an hour after the test.	Other: Stress test; Stress test while running on a treadmill until exhaustion for 8-12 minutes. Blood will be drawn before, 5 minutes after and an hour after the test.; Other Names: blood test

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Time Frame
Higher increase in oxidative stress inducing factors, and antioxidants in men with lower physical fitness compared with men with higher physical fitness.	one year

Collaborators and Investigators

• Sponsor: Wingate Institute
• Collaborators: Tel Aviv University
• Investigators: Study Chair: Dov Lichtenberg, PhD, Tel Aviv University

Study record dates

Study Major Dates

• Study Start: June 1, 2011
• Primary Completion (ACTUAL): May 1, 2012
• Study Completion (ACTUAL): May 1, 2012

Study Registration Dates

• First Submitted: February 3, 2011
• First Submitted That Met QC Criteria: February 13, 2011
• First Posted (ESTIMATE): February 15, 2011

Study Record Updates

• Last Update Posted (ESTIMATE): January 1, 2014
• Last Update Submitted That Met QC Criteria: December 31, 2013
• Last Verified: December 1, 2013

More Information

Terms related to this study

• Keywords: oxidative stress, physical exercise, fitness level
• Other Study ID Numbers: Wingate 05