Antioxidants Vitamins and Muscle Damage (Antioxidant)

Determine if acute supplementation with antioxidant vitamins (vitamin C and vitamin E) influences the muscle damage and fatigue caused after an acute session of eccentric training.

Study Overview

• Status: Completed
• Conditions: Oxidative Stress
• Intervention / Treatment: Dietary supplement: Dietary Supplement: Vitamin C and vitamin E; Dietary supplement: Placebo

Detailed Description

A randomized, single-blind, placebo-control parallel trial will be conducted. In a previous session volunteers will be interviewed to determine if they meet the inclusion criteria.

Participants should avoid ingesting of foods that contain high amounts of antioxidant compounds: more than two juices of fruits, more than four cups of coffee or tea. Juices with high antioxidant content should be avoided. Alcoholic beverages should also be avoided. Participants will be divided in to groups and will performance one resistance exercise sessions. Prior to exercise, one group will intake supplementation VitC (1000 mg) + VitE (235 mg) and the other supplementation with placebo.

The following sequence will be performed in each session: 1) baseline assessment (analysis of the composition body, blood draw and parameters of performance) prior to exercise session ; 2) administration of vitamin or placebo supplementation 2 hours before exercise session ; 3) post-exercise assessments (blood biomarkers and performance parameters) immediately after the session and after 24 hours. DOMS will also be assessed 48 h and 72 h after training. The training session implemented will be designed by a Sport Scientific

• Study Type: Interventional
• Enrollment (Actual): 18
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Spain
  • Madrid, Spain
    • Universidad Europea de Madrid

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 33 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: All

Description

Inclusion Criteria:

• Healthy
• Physically active

Exclusion Criteria:

• Injuries
• Supplementation or drugs
• Smokers

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Treatment
• Allocation: Randomized
• Interventional Model: Parallel Assignment
• Masking: Quadruple

Number of Arms

2

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Supplementation; Vitamin C and Vitamin E before exercise	Dietary supplement: Dietary Supplement: Vitamin C and vitamin E; Antioxidant supplementation; Other Names: Ascorbic acid and tocopherol
Placebo Comparator: Placebo; Placebo before exercise	Dietary supplement: Placebo; Placebo administration

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Percentage of body fat and muscle mass	Determination of body composition using skinfolds measurements	Prior to the intervention
DOMS	Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain)	0 hours post exercise
DOMS	Delayed onset muscle soreness. This outcome will be measure with a 10-point visual analogic scale (0= no pain; 10 = worst possible pain)	24 hours post exercise
Blood malondialdehyde (MDA)	MDA will be analyzed from a blood sample	2 hours prior to exercise
Blood creatine quinase (CK)	CK will be analyzed from a blood sample	2 hours prior to exercise
Blood malondialdehyde (MDA)	MDA will be analyzed from a blood sample	Inmediatly after exercise
Blood malondialdehyde (MDA)	MDA will be analyzed from a blood sample	24 hours after exercise
Blood creatine quinase (CK)	CK will be analyzed from a blood sample	24 hours after exercise
Jump height in a Counter Movement Jump	Jump height (cm) in a counter movement jump measured with Octojump Platform	2 hours prior to exercise
Jump height in a Counter Movement Jump	Jump height (cm) in a counter movement jump measured with Octojump Platform	Inmediatly after exercise
Jump height in a Counter Movement Jump	Jump height (cm) in a counter movement jump measured with Octojump Platform	24 h after exercise
Jump Height in a Squat Jump	Jump Height (cm) in a Squat Jump measured with Octojump Platform	24 h after exercise
Jump Height in a Squat Jump	Jump Height (cm) in a Squat Jump measured with Octojump Platform	Inmediately after exercise
Jump Height in a squat Jump	Jump height (cm) in a squat jump with Octojump Platform measured with Octojump Platform	2 hours prior to exercise
Jump Height in a Drop Jump	Jump height (cm) was measured in different drop jumps with Octojump Platform	2 hours prior to exercise
Jump Height in a Drop Jump	Jump height (cm) was measured in different drop jumps with Octojump Platform	Inmediately after exercise
Contact time in a Drop Jump	Contact time (s)measured in different drop jumps with Octojump Platform	Inmediately after exercise
Jump Height in a Drop Jump	Jump height (cm) measured in different drop jumps with Octojump Platform	Inmediately after exercise
Contact time in a Drop Jump	Contact time (s) measured in different drop jumps with Octojump Platform	24 hours after exercise
Jump Height in a Drop Jump	Jump height (cm) measured in different drop jumps with Octojump Platform	24 hours after exercise
Contact time in a Drop Jump	Capillary blood lactate evaluated with Lactate Pro 2	Inmediately after exercise
Concentration of blood lactate	Capillary blood lactate with Lactate Pro 2	2 hours before exercise

Collaborators and Investigators

• Sponsor: Universidad Europea de Madrid

Study record dates

Study Major Dates

• Study Start (Actual): February 10, 2022
• Primary Completion (Actual): February 15, 2022
• Study Completion (Actual): March 6, 2022

Study Registration Dates

• First Submitted: June 15, 2021
• First Submitted That Met QC Criteria: November 8, 2021
• First Posted (Actual): November 19, 2021

Study Record Updates

• Last Update Posted (Actual): April 7, 2022
• Last Update Submitted That Met QC Criteria: April 4, 2022
• Last Verified: April 1, 2022

More Information

Terms related to this study

• Keywords: oxidative damage; antioxidant vitamins
• Additional Relevant MeSH Terms: Physiological Effects of Drugs; Molecular Mechanisms of Pharmacological Action; Protective Agents; Micronutrients; Antioxidants; Vitamin E; Tocopherols; Vitamins; Ascorbic Acid
• Other Study ID Numbers: CIPI/20/209-part2

Plan for Individual participant data (IPD)

• Plan to Share Individual Participant Data (IPD)?: NO

Drug and device information, study documents

• Studies a U.S. FDA-regulated drug product: No
• Studies a U.S. FDA-regulated device product: No