Effects of Exercise on Fructose Metabolism (FruEx2)

A high fructose diet increases fasting and post-prandial triglyceride (TG) concentrations in sedentary healthy human subjects.These effects may be secondary to fructose-induced hepatic de novo lipogenesis. Recent evidence indicate that exercise can prevent fructose induced dyslipidemia.This study will evaluate

1. how exercise effects the metabolic fate of oral fructose 1a) when exercise is performed before fructose ingestion 1b) when exercise is performed after fructose ingestion Metabolic effects of exercise will be assessed in healthy male subjects by measuring fructose oxidation (13CO2 production), fructose conversion into glucose (13C glucose concentrations in blood) and hepatic fructose conversion into lipid (13C palmitate-very low density lipoprotein (VLDL) concentrations in blood) after ingestion of 13C-labelled fructose meals
2. how fructose and protein modulate muscle glycogen and intramyocellular lipid repletion after exercise Healthy male subjects will be fed various fructose, glucose, lipid and whey protein meals after a glycogen/intramyocellular lipid depleting exercise. The effects of meals' composition will be assessed after 24 hours by measuring intramyocellular lipids and glycogen using proton-magnetic resonance spectroscopy (MRS).

Study Overview

• Status: Completed
• Conditions: Healthy Subjects
• Intervention / Treatment: Other: exercise; Dietary supplement: fructose; Dietary supplement: glucose

• Study Type: Interventional
• Enrollment (Actual): 8
• Phase: Not Applicable

Contacts and Locations

Study Locations

• Switzerland
  • Vaud
    • Lausanne, Vaud, Switzerland, CH-1011
      • Centre Hospitalier Universitaire Vaudois

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

Description

Inclusion Criteria:

• males
• 18-40 years old
• 19 kg/m2>BMI>25kg/m2
• moderate to high usual physical activity
• non-smokers

Exclusion Criteria:

• family history of diabetes mellitus
• ECG anomaly
• any medication
• participation to blood spending or other clinical study in the 3 months before the beginning of this study
• consumption of drugs
• consumption of more than 50g alcool/week
• family history of food intolerance

Study Plan

How is the study designed?

Design Details

• Primary Purpose: Basic Science
• Allocation: Randomized
• Interventional Model: Crossover Assignment
• Masking: None (Open Label)

Number of Arms

5

Arms and Interventions

Participant Group / Arm	Intervention / Treatment
Experimental: Study1a; exercice performed 90 min before 13C fructose meal ingestion	Other: exercise; cycling at 100W during 60 min
Experimental: study 1b; exercise performed 90 min after 13C fructose meal ingestion	Other: exercise; cycling at 100W during 60 min
No Intervention: study 1c; no exercise
Experimental: study 2a; meals containing fructose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise	Dietary supplement: fructose; isocaloric nutrition with fructose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise
Active Comparator: study 2b; meals containing glucose, cream and whey proteins over 24 hours after a glycogen/intramyocellular lipid depleting exercise	Dietary supplement: glucose; isocaloric nutrition with glucose, cream and whey protein during the 24 hour following a glycogen/intramyocellular lipid depleting exercise

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Plasma triglyceride	measurement of total and VLDL-TG concentrations in study 1	measurements in fasting conditions and every hour from 7:00 am to 4:00 pm (up to 9 hours)
intramyocellular lipids	Measurement by 1H-MRS in muscle vastus lateralis in study 2	after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise
Fructose conversion into lipids	Estimated from 13C palmitate-VLDL concentration	measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Fructose conversion into glucose	measurements obtained from plasma 13C glucose concentrations and whole body glucose production measured by 6,6 d2 glucose in study 1)	measured between 7:00 am and 4:oo pm (up to 9 hours)
intramyocellular glycogen concentrations	Measured by 1H-MRS in muscle vastus lateralis in study 2	after glycogen/intramyocellular lipid depleting exercise and after 24 hour controlled feeding post exercise
fructose oxidation	Calculated from breath 13CO2 production	measured from 7:00 am to 4:00 pm in study 1 (up to 9 hours)
exogenous lipid oxidation	Lipids ingested will be labeled with 13C-triolein, and exogenous lipid oxidation will be measured from breath 13CO2 production	measured from 7:00 am to 4:00 pm in study 2 (up to 9 hours)

Collaborators and Investigators

• Sponsor: University of Lausanne
• Collaborators: Centre Hospitalier Universitaire Vaudois; University of Bern

Publications and helpful links

General Publications

• Rosset R, Lecoultre V, Egli L, Cros J, Dokumaci AS, Zwygart K, Boesch C, Kreis R, Schneiter P, Tappy L. Postexercise repletion of muscle energy stores with fructose or glucose in mixed meals. Am J Clin Nutr. 2017 Mar;105(3):609-617. doi: 10.3945/ajcn.116.138214. Epub 2017 Jan 18.
• Egli L, Lecoultre V, Cros J, Rosset R, Marques AS, Schneiter P, Hodson L, Gabert L, Laville M, Tappy L. Exercise performed immediately after fructose ingestion enhances fructose oxidation and suppresses fructose storage. Am J Clin Nutr. 2016 Feb;103(2):348-55. doi: 10.3945/ajcn.115.116988. Epub 2015 Dec 23.

Study record dates

Study Major Dates

• Study Start: May 1, 2013
• Primary Completion (Actual): January 1, 2014
• Study Completion (Actual): January 1, 2014

Study Registration Dates

• First Submitted: May 27, 2013
• First Submitted That Met QC Criteria: May 30, 2013
• First Posted (Estimate): May 31, 2013

Study Record Updates

• Last Update Posted (Estimate): June 4, 2014
• Last Update Submitted That Met QC Criteria: June 3, 2014
• Last Verified: June 1, 2014

More Information

Terms related to this study

• Keywords: fructose; gluconeogenesis; de novo lipogenesis; intramyocellular lipids; muscle glycogen
• Other Study ID Numbers: University of Lausanne