The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

February 18, 2014 updated by: Hillel Yaffe Medical Center

The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization Outcome of Sibling Oocytes

The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Study Overview

Status

Unknown

Conditions

Intervention / Treatment

Detailed Description

This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media:

  1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult)
  2. P1 medium vs. ECM medium (Irvine).

Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Study Type

Observational

Enrollment (Anticipated)

600

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Locations

  • Israel
      • Hadera, Israel, 38100
        • Hillel Yaffe Medical Center

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 42 years (Adult)

Accepts Healthy Volunteers

No

Genders Eligible for Study

Female

Sampling Method

Probability Sample

Study Population

Sibling oocytes retrieved from women who have undergone IVF treatment in our IVF unit.

Description

Inclusion Criteria:

  • Sibling oocytes from cycles where at least 4 oocytes were retrieved

Exclusion Criteria:

  • Less than 4 oocytes retrieved
  • Partner has undergone testicular sperm extraction (TESE)

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

Cohorts and Interventions

Group / Cohort
Intervention / Treatment
Universal IVF Medium
An oocyte culture medium containing 5.55 Mm glucose (Origio, Medi-Cult)
Other: Sibling oocyte incubation
ISM1 Medium
An oocyte culture medium containing 1mM glucose ((Origio, MediCult)
Other: Sibling oocyte incubation
P1 Medium
An oocyte culture medium containing no glucose (Irvine)
Other: Sibling oocyte incubation
ECM Medium
An oocyte culture medium containing 0.5mM glucose (Irvine)
Other: Sibling oocyte incubation

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Fertilization Rate
Time Frame: 2 years
Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated
2 years

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Embryo Quality
Time Frame: 2 years
Embryo quality and developmental rate will be retrospectively compared according to culture media in which sibling oocytes were incubated
2 years

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Hillel Yaffe Medical Center

Investigators

  • Principal Investigator: Medeia Michaelo, PhD, Hellel Yaffe Medical Center

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start

April 1, 2014

Primary Completion (Anticipated)

April 1, 2016

Study Completion (Anticipated)

April 1, 2016

Study Registration Dates

First Submitted

February 16, 2014

First Submitted That Met QC Criteria

February 18, 2014

First Posted (Estimate)

February 19, 2014

Study Record Updates

Last Update Posted (Estimate)

February 19, 2014

Last Update Submitted That Met QC Criteria

February 18, 2014

Last Verified

February 1, 2014

More Information

Terms related to this study

Keywords

Additional Relevant MeSH Terms

Other Study ID Numbers

  • 0011-14HYMC

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

Clinical Trials on Infertility

Clinical Trials on Sibling oocyte incubation

Search Similar Trials

Sponsors and Collaborators

Medical Conditions

Drug Interventions

CROs by country

CROs in Israel

Conditions

Rare Diseases

Drug Interventions

Dietary Supplements

Sponsor/Collaborators

Locations

Subscribe