Examining Endogenous Antioxidant Levels in Well Trained Cyclists

The Effects of Habitual Antioxidant Supplementation on Endogenous Antioxidants and Oxidative Damage in Well Trained Cyclists

This study will investigate whether increased habitual intake of antioxidant supplements effects the concentration of endogenous antioxidants, the response of antioxidants to exercise, and differences in oxidative damage compared to a non-supplemented group.

Study Overview

• Status: Completed
• Conditions: Oxidative Stress

• Study Type: Observational
• Enrollment (Actual): 14

Contacts and Locations

Study Locations

• United Kingdom
  • West Midlands
    • Birmingham, West Midlands, United Kingdom, B15 2TT
      • School of Sport Exercise and Rehabilitation Sciences, University of Birmingham

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 35 years (Adult)
• Accepts Healthy Volunteers: Yes
• Genders Eligible for Study: Male

• Sampling Method: Non-Probability Sample

Study Population

Cyclist from the local West Midlands (United Kingdom) area will be invited to take part in the study.

Description

Inclusion Criteria:

• Male,
• BMI 18.8 29.9 kg/m2
• cycle 3-7 times a week for durations longer than 60 minutes
• cycled for a minimum of 3 years
• accustomed to exhaustive cycling protocols
• healthy (no known cardiovascular or metabolic disease)

Exclusion Criteria:

• answering "NO" to any question on the general health questionnaire would result in exclusion from the study
• current participation in another clinical study
• current or recent smoker (last 30 days)
• prescription or non-prescription of anti-inflammatory use >30 days prior to the experiment commencing or any other medications that in the opinion of the investigator may interfere with redox signalling

Study Plan

How is the study designed?

Number of groups / cohorts

2

Cohorts and Interventions

Group / Cohort
Supplemented; The group consists of well-trained cyclists who habitually consume vitamin/antioxidant supplements for a period longer than 6 months
Non-supplemented; The group consists of well-trained cyclists who do not consume vitamin/antioxidant supplements

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
Difference in Resting Levels of Endogenous Antioxidant Proteins in Skeletal Muscle - Superoxide Dismutase 2 (SOD-2)	To examine the effects of habitual vitamin and antioxidant supplementation on endogenous antioxidant production, participants will have a muscle biopsy taken at rest to examine any differences in endogenous antioxidant protein concentrations	Protein concentrations will be measured at baseline only

Collaborators and Investigators

• Sponsor: University of Birmingham
• Investigators: Principal Investigator: Sarah Aldred, MSc PhD, University of Birmingham

Study record dates

Study Major Dates

• Study Start (Actual): July 1, 2013
• Primary Completion (Actual): May 1, 2016
• Study Completion (Actual): May 1, 2016

Study Registration Dates

• First Submitted: October 16, 2014
• First Submitted That Met QC Criteria: October 30, 2014
• First Posted (Estimate): November 4, 2014

Study Record Updates

• Last Update Posted (Actual): August 11, 2020
• Last Update Submitted That Met QC Criteria: August 7, 2020
• Last Verified: August 1, 2020

More Information

Terms related to this study

• Keywords: Oxidative stress; Vitamin Supplements; Endogenous antioxidants; Antioxidant supplements; Exercise adaptations; Redox regulation; Cycling - exercise
• Other Study ID Numbers: 125663; 13/WM/0226 (Other Identifier: NRES Committee West Midlands - Solihull)