Chronic Periodontitis and Polycystic Ovary Syndrome

Evaluation Of Lipid Peroxidation, Oxidative DNA Damage And Total Antioxidant Status In Patients With Periodontitis And Polycystic Ovary Syndrome: A Cross Sectional Study

Background: The aim of this study was to investigate the levels of malondialdehyde (MDA), total antioxidant status (TAS), 8-hydroxy-2´- deoxyguanosine (8-OHdG) in blood serum and saliva samples in female patients with chronic periodontitis (CP) and to compared healthy individuals.

Materials and Methods: A total of 88 women were into four groups each which were consisted of twenty-two subjects. Groups were designed as periodontally and systemically healthy women (PCOS-CP-); periodontally healthy women with polycystic ovary syndrome (PCOS) (PCOS+CP-), systemically healthy women with CP (PCOS-,CP+) and women with PCOS and CP (PCOS+CP+). Serum and salivary samples were obtained, clinical periodontal parameters were recorded. MDA, TAS, 8-OHdG levels were measured as biochemically.

Study Overview

• Status: Completed
• Conditions: Polycystic Ovary Syndrome; Chronic Periodontitis
• Intervention / Treatment: Other: no intervention provided

Detailed Description

RESULTS:

Salivary 8-OHdG levels in the PCOSCP and CP groups were statistically higher than those in both the PCOSPH and the PH groups (P < 0.05). There was no statistical difference between the PCOSCP, CP, and PCOSPH groups with regard to salivary MDA and TAS levels (P > 0.05). Highest serum 8-OHdG and MDA levels and lowest serum TAS levels were seen in the PCOSCP group (P < 0.05). Serum 8-OHdG and MDA levels in the PCOSPH group were higher than those in both systemically healthy groups (PH and CP) (P < 0.05). Salivary TAS levels were highest (P < 0.05) in the PH group. There was no statistical difference between the CP and PCOSPH groups, but serum TAS levels were lower than those in the PH group (P < 0.05).

CONCLUSIONS:

CP, which led to an increase in serum and salivary 8-OHdG and MDA levels and a decrease in serum TAS levels in patients with PCOS, contributed to increased OS. This effect was more prominent in serum levels than in salivary levels.

• Study Type: Observational
• Enrollment (Actual): 88

Contacts and Locations

Study Locations

• Turkey
  • Fatih
    • İstanbul, Fatih, Turkey, 34093
      • Department of Periodontics, Faculty of Dentistry, Bezmialem Vakif University

Participation Criteria

Eligibility Criteria

• Ages Eligible for Study: 18 years to 40 years (Adult)
• Accepts Healthy Volunteers: No
• Genders Eligible for Study: Female

• Sampling Method: Non-Probability Sample

Study Population

All participants were recruited among women at the Department of Obstetrics and Gynecology, School of Medicine, Ataturk University

Description

Inclusion Criteria:

• Newly diagnosed non-obese PCOS patients
• Never smokers
• Had no history of systemic disease
• BMI<25 kg/m2
• Participants had ≥20 teeth present.

Exclusion Criteria:

• Pregnancy
• Lactation
• Hemoglobin A1c (HbA1c) ≥ 6.5%
• 2-h oral glucose tolerance test (OGTT-2h) ≥200
• Cushing syndrome, non-classic congenital adrenal hyperplasia, hyperprolactinemia, thyroid dysfunction, and androgen-secreting tumors
• Any drug use within the past 6 months
• Periodontal treatment within the past 6 months

Study Plan

How is the study designed?

Design Details

• Observational Models: Case-Control
• Time Perspectives: Cross-Sectional

Number of groups / cohorts

4

Cohorts and Interventions

Group / Cohort	Intervention / Treatment
(PCOS-CP-); systemically and periodontally healthy participants	Other: no intervention provided
(PCOS-CP+); systemically healthy participants with CP	Other: no intervention provided
(PCOS+CP-); PCOS participants with periodontally healthy	Other: no intervention provided
(PCOS+CP+); PCOS participants with CP	Other: no intervention provided

What is the study measuring?

Primary Outcome Measures

Outcome Measure	Measure Description	Time Frame
8-OHdG levels (nanogram per mililiter(ng/mL))	by ELISA test	appoximately 2 years
MDA levels (micromole per liter (µmol/L))	by ELISA test	appoximately 2 years
TAS levels (millimolar per liter (mmol /L))	by ELISA test	appoximately 2 years
Plaque Index (PI)	Silnes-Löe Periodondal İndex	appoximately 2 years
Gingival Index (GI)	Löe-Silness Periodontal Index	appoximately 2 years
Bleeding on probing (BOP)	Gİngival Bleeding Index	appoximately 2 years
Clinical attachment level (CAL)	by measuring the distance between the cementoenamel junction and the periodontal pocket base	Patients were seen one time
Probing pocket depth (PD))	distance between free gingival margin and periodontal pocket base by usin periodontal probe	appoximately 2 years

Secondary Outcome Measures

Outcome Measure	Measure Description	Time Frame
number of individuals with chronic periodontitis	individuals in the group	appoximately 2 years
numberer of individuals with polycystic ovary syndrome	individuals in the group	appoximately 2 years

Collaborators and Investigators

• Sponsor: Bezmialem Vakif University
• Investigators: Principal Investigator: Nesrin Saruhan, PhD, Eskisehir Osmangazi Universitesi; Study Director: Cenk Fatih Çanakcı, Prof, Atatürk Üniversitesi; Principal Investigator: Saime Ozbek Sebin, Dr, Atatürk Üniversitesi; Principal Investigator: Humeyra Canakcı, Dr, Buhara Hastanesi; Principal Investigator: Metin İngeç, Prof, Atatürk Üniversitesi; Principal Investigator: Ufuk Sezer, Phd, Özel Klinik; Principal Investigator: Ebru Saglam, PhD, Saglik Bilimleri Universitesi

Study record dates

Study Major Dates

• Study Start: May 1, 2013
• Primary Completion (Actual): May 1, 2015
• Study Completion (Actual): September 1, 2015

Study Registration Dates

• First Submitted: October 28, 2016
• First Submitted That Met QC Criteria: November 1, 2016
• First Posted (Estimate): November 3, 2016

Study Record Updates

• Last Update Posted (Actual): January 18, 2020
• Last Update Submitted That Met QC Criteria: January 13, 2020
• Last Verified: January 1, 2020

More Information

Terms related to this study

• Keywords: Malondialdehyde; 8- hydroxy -2´- deoxyguanosine; Total antioxidant status; Oxidative stres
• Additional Relevant MeSH Terms: Pathologic Processes; Neoplasms; Endocrine System Diseases; Disease; Ovarian Cysts; Cysts; Ovarian Diseases; Adnexal Diseases; Gonadal Disorders; Stomatognathic Diseases; Periodontal Diseases; Mouth Diseases; Polycystic Ovary Syndrome; Syndrome; Periodontitis; Chronic Periodontitis
• Other Study ID Numbers: PCOSandCP