Fluorescent Hand Hygiene Training and Long-Term Effects in First-Year Nursing Students
August 10, 2026 updated by: Powiślańska Academy of Applied Sciences
Durability of Fluorescent Hand Hygiene Training Effects Among First-Year Nursing Students: A Single-Group Quasi-Experimental Before-After Study With Repeated Measures of Knowledge and Technique
This single-group quasi-experimental study will evaluate the effectiveness and durability of a fluorescent hand hygiene training program for first-year nursing students at a Polish university.
Students will complete the WHO Hand Hygiene Knowledge Questionnaire and undergo Glo Germ-based fluorescent assessment of handwashing technique at baseline, immediately after the training, approximately two weeks later, at the end of the first semester, and just before their first clinical placements.
The primary objective is to determine whether the training improves and sustains hand hygiene technique quality over time, while secondary objectives include changes in hand hygiene knowledge and the relationship between knowledge and technique
Study Overview
Status
Not yet recruiting
Intervention / Treatment
Study Type
Interventional
Enrollment (Estimated)
80
Phase
- Not Applicable
Participation Criteria
Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.
Eligibility Criteria
Ages Eligible for Study
- Adult
Accepts Healthy Volunteers
Yes
Description
Inclusion Criteria:
- First-year nursing students enrolled at Powiślańska Academy of Applied Sciences in Kwidzyn during the study period.
- Aged 18 years or older.
- Able to provide written informed consent.
- Able to participate in the planned educational activities.
Exclusion Criteria:
- Known allergy or skin intolerance to Glo Germ™ /gel or similar products.
- Current dermatological condition of the hands that precludes the use of fluorescent lotion/gel or repeated handwashing (e.g., severe eczema or open wounds).
- Previous participation in a pilot study or an earlier version of the same fluorescent hand-hygiene training at this institution.
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Design Details
- Primary Purpose: Prevention
- Allocation: N/A
- Interventional Model: Single Group Assignment
- Masking: None (Open Label)
Arms and Interventions
Participant Group / Arm |
Intervention / Treatment |
|---|---|
|
Experimental: Fluorescent Hand Hygiene Training Arm
|
First-year nursing students receive a standardized fluorescent hand hygiene training session using Glo Germ lotion and UV light.
The session includes a brief review of WHO hand hygiene indications, demonstration of proper handwashing technique, application of fluorescent lotion, handwashing by the student, and visual feedback of missed areas under UV illumination.
Students repeat handwashing until adequate coverage is achieved.
This training is delivered early in the first semester and its effects are assessed at immediate, short-term, semester-end, and pre-clinical time points
|
What is the study measuring?
Primary Outcome Measures
Outcome Measure |
Measure Description |
Time Frame |
|---|---|---|
|
Quality of hand hygiene technique assessed with fluorescent Glo Germ
Time Frame: Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements
|
Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements
|
|
|
Quality of hand hygiene technique assessed with fluorescent Glo Germ
Time Frame: Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements
|
Hand hygiene technique will be evaluated at five time points using Glo Germ fluorescent lotion and UV light.
After applying fluorescent lotion, students wash their hands as usual; residual fluorescence indicating missed areas is recorded by the investigator on a standardized checklist (e.g., fingertips, thumbs, interdigital spaces, dorsal surfaces).
The primary outcome will be the proportion of students achieving adequate technique (no or minimal missed areas) and changes in individual technique scores over time
|
Baseline, immediately post-training, approximately 2 weeks post-training, end of first semester, and just before first clinical placements
|
Collaborators and Investigators
This is where you will find people and organizations involved with this study.
Study record dates
These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.
Study Major Dates
Study Start (Estimated)
October 15, 2026
Primary Completion (Estimated)
June 30, 2027
Study Completion (Estimated)
June 30, 2027
Study Registration Dates
First Submitted
August 3, 2026
First Submitted That Met QC Criteria
August 10, 2026
First Posted (Actual)
August 11, 2026
Study Record Updates
Last Update Posted (Actual)
August 11, 2026
Last Update Submitted That Met QC Criteria
August 10, 2026
Last Verified
July 1, 2026
More Information
Terms related to this study
Other Study ID Numbers
- PANS-HH-EDU-2026
- 1/2026 (Powiślańska Akademia Nauk Stosowanych w Kwidzynie)
Drug and device information, study documents
Studies a U.S. FDA-regulated drug product
No
Studies a U.S. FDA-regulated device product
No
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