Effect of Ascorbic Acid-Loaded I-PRF With Partially Demineralized Dentin Graft on Alveolar Ridge Preservation (AA-IPRF-PDDG)

October 31, 2026 updated by: Mahmoud Ebrahim Ahmed Elsaadawy, Nahda University

Effect of Ascorbic Acid-Loaded Injectable Platelet-Rich Fibrin Combined With Partially Demineralized Dentin Graft on Alveolar Ridge Preservation: A Randomized Controlled Clinical Trial

The goal of this clinical trial is to find out which treatment best preserves the jawbone after tooth extraction in adults planning to receive a dental implant later.

The study will investigate whether adding vitamin C to a mixture of tooth particles and a material prepared from the participant's own blood helps preserve the jawbone and improve bone healing.

The main questions it aims to answer are

  • Does adding vitamin C help preserve the width of the jawbone after tooth extraction?
  • Does it improve new bone formation compared with the other treatments?

Researchers will compare three treatments to determine whether adding a blood-based material, with or without vitamin C, improves bone preservation.

  1. The first group will receive small particles prepared from their own extracted tooth.
  2. The second group will receive tooth particles mixed with a healing material prepared from their own blood.
  3. The third group will receive tooth particles mixed with the same blood-based material containing vitamin C.

Participants will undergo tooth extraction and receive one of these treatments. They will attend follow-up visits during the healing period.

Researchers will:

  • Examine the treated area during follow-up visits.
  • Take three-dimensional dental scans before treatment and four months later to measure changes in jawbone width and height.
  • Collect a small bone sample during dental implant placement to examine new bone formation and healing.

The study will help determine whether adding vitamin C to the blood-based material provides additional benefits for preserving the jawbone before dental implant placement.

Study Overview

Status

Not yet recruiting

Conditions

Intervention / Treatment

Detailed Description

This study evaluates approaches to preserve the alveolar ridge following tooth extraction before delayed dental implant placement.

The study uses a partially demineralized dentin graft prepared from the participant's extracted tooth as the grafting material. It also investigates whether combining this graft with injectable platelet-rich fibrin (I-PRF), with or without added ascorbic acid (vitamin C), may influence bone healing and ridge preservation.

The rationale for using dentin is based on its potential as an autologous graft material. I-PRF provides an autologous blood-derived component. The addition of vitamin C is being investigated as a possible way to influence the healing response.

The study will assess changes in alveolar ridge dimensions and examine bone tissue collected during implant placement. Histological and histomorphometric analysis will help characterize new bone formation, residual graft material, and connective tissue within the healing site.

The findings may provide information about the role of vitamin C-enriched I-PRF when combined with autogenous dentin grafts for alveolar ridge preservation.

Study Type

Interventional

Enrollment (Estimated)

48

Phase

  • Not Applicable

Contacts and Locations

This section provides the contact details for those conducting the study, and information on where this study is being conducted.

Study Contact

Study Contact Backup

Participation Criteria

Researchers look for people who fit a certain description, called eligibility criteria. Some examples of these criteria are a person's general health condition or prior treatments.

Eligibility Criteria

Ages Eligible for Study

18 years to 60 years (Adult)

Accepts Healthy Volunteers

Yes

Genders Eligible for Study

All

Description

Inclusion Criteria:

  • Patients aged 18-60 years. ⁴⁷
  • ASA Type I medically healthy patients with no significant medical history. ⁴⁷
  • Non-restorable teeth indicated for extraction followed by delayed implant placement. ⁴⁸
  • Patients eligible for immediate implant placement but facing factors that prevent implant placement at the time of extraction. ⁴⁸
  • Patients requiring alveolar ridge preservation before planned dental implant placement.
  • Presence of ST IB sockets after extraction. ⁴²-⁴⁴
  • periodontally healthy adjacent teeth. ⁴⁸
  • Adequate oral hygiene plaque index ≤15% and willingness to comply with the study protocol and follow-up visits. ⁴⁷
  • Cooperative patients willing to comply with the 4-month follow-up period. ⁴⁷
  • Ability to understand and sign the informed consent form. ⁴⁷

Exclusion Criteria:

  • Uncontrolled systemic diseases affecting bone healing (e.g., uncontrolled diabetes mellitus).
  • History of radiotherapy or chemotherapy involving the head and neck region.
  • Current use of medications known to influence bone metabolism (e.g., bisphosphonates, denosumab, long-term corticosteroids).
  • Smokers or tobacco users.
  • Pregnancy or lactation.
  • Acute uncontrolled infection extraction site.
  • Patients with poor oral hygiene or untreated active periodontal disease.
  • Psychiatric patients, or those with a learning disability, or those unable to give consent
  • Patients unable or unwilling to attend follow-up visits.

Study Plan

This section provides details of the study plan, including how the study is designed and what the study is measuring.

How is the study designed?

Design Details

  • Primary Purpose: Treatment
  • Allocation: Randomized
  • Interventional Model: Parallel Assignment
  • Masking: Single

Arms and Interventions

Participant Group / Arm
Intervention / Treatment
Active Comparator: Partially Demineralized Dentin Graft Alone

Participants will undergo alveolar ridge preservation following atraumatic tooth extraction using an autogenous partially demineralized dentin graft (PDDG) prepared from the extracted tooth. The graft will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures. No injectable platelet-rich fibrin (I-PRF) or ascorbic acid will be added.
Procedure: Alveolar Ridge Preservation Using Partially Demineralized Dentin Graft alone

Alveolar ridge preservation following atraumatic tooth extraction using an autogenous partially demineralized dentin graft (PDDG) prepared from the extracted tooth. The graft will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures. No injectable platelet-rich fibrin (I-PRF) or ascorbic acid will be added.
Experimental: Partially Demineralized Dentin Graft Combined with I-PRF

Participants will undergo alveolar ridge preservation using an autogenous partially demineralized dentin graft (PDDG) mixed with injectable platelet-rich fibrin (I-PRF) prepared from the participant's peripheral blood. The resulting mixture, referred to as Sticky Dentin, will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures.
Procedure: Alveolar Ridge Preservation Using PDDG Combined with I-PRF

Alveolar ridge preservation using an autogenous partially demineralized dentin graft (PDDG) mixed with injectable platelet-rich fibrin (I-PRF) prepared from the participant's peripheral blood. The resulting sticky dentin mixture will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures.
Experimental: Ascorbic Acid-Loaded I-PRF Combined with PDDG

Participants will undergo alveolar ridge preservation using an autogenous partially demineralized dentin graft (PDDG) mixed with injectable platelet-rich fibrin (I-PRF) containing ascorbic acid. The resulting mixture, referred to as AA-Sticky Dentin, will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures.
Procedure: Alveolar Ridge Preservation Using PDDG Combined with Ascorbic Acid-Loaded I-PRF

Alveolar ridge preservation using an autogenous partially demineralized dentin graft (PDDG) mixed with injectable platelet-rich fibrin (I-PRF) containing ascorbic acid. The resulting AA-Sticky Dentin mixture will be placed into the extraction socket, covered with an absorbable gelatin sponge, and secured with sutures.

What is the study measuring?

Primary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Change in Radiographic Horizontal Alveolar Ridge Width
Time Frame: Baseline (before alveolar ridge preservation) to 4 months postoperatively.

The change in horizontal alveolar ridge width will be assessed using cone-beam computed tomography (CBCT) scans obtained at baseline before alveolar ridge preservation and 4 months postoperatively. Measurements will be performed at the extraction site using standardized anatomical reference points and consistent measurement procedures. The change in ridge width from baseline to 4 months will be compared among the three study groups.
Baseline (before alveolar ridge preservation) to 4 months postoperatively.

Secondary Outcome Measures

Outcome Measure
Measure Description
Time Frame
Histomorphometric Analysis of Core Bone Biopsy Specimens
Time Frame: 4 months after alveolar ridge preservation, during implant osteotomy. 4 months after alveolar ridge preservation, during implant osteotomy.

Histomorphometric analysis will be performed on core bone biopsy specimens collected from the alveolar ridge preservation sites during implant osteotomy 4 months postoperatively. The analysis will assess the percentages of newly formed bone, mature bone, and residual dentin graft particles in the specimens, and the results will be compared among the three study groups.
4 months after alveolar ridge preservation, during implant osteotomy. 4 months after alveolar ridge preservation, during implant osteotomy.

Collaborators and Investigators

This is where you will find people and organizations involved with this study.

Sponsor

Nahda University

Investigators

  • Study Chair: Mohamed Sherif EL-Mofty, Professor, Faculty of Dentistry, Nahda University Beni-Suef, Egypt.
  • Study Director: Shaimaa Hamdy Abd El-Azeem, : Department of Oral Medicine, Diagnosis and Periodontology, Faculty of Dentistry, Nahda University Beni-Suef, Egypt.

Publications and helpful links

The person responsible for entering information about the study voluntarily provides these publications. These may be about anything related to the study.

General Publications

Study record dates

These dates track the progress of study record and summary results submissions to ClinicalTrials.gov. Study records and reported results are reviewed by the National Library of Medicine (NLM) to make sure they meet specific quality control standards before being posted on the public website.

Study Major Dates

Study Start (Estimated)

October 30, 2026

Primary Completion (Estimated)

March 30, 2027

Study Completion (Estimated)

June 30, 2027

Study Registration Dates

First Submitted

October 17, 2026

First Submitted That Met QC Criteria

October 31, 2026

First Posted (Actual)

November 4, 2026

Study Record Updates

Last Update Posted (Actual)

November 4, 2026

Last Update Submitted That Met QC Criteria

October 31, 2026

Last Verified

October 1, 2026

More Information

Terms related to this study

Keywords

Additional Relevant MeSH Terms

Other Study ID Numbers

  • 00D-9-26-8

Plan for Individual participant data (IPD)

Plan to Share Individual Participant Data (IPD)?

NO

IPD Plan Description

Individual participant data will not be shared with other researchers. Only aggregated study results may be disseminated through scientific publications, while participant confidentiality and data privacy will be maintained in accordance with institutional ethical requirements.

Drug and device information, study documents

Studies a U.S. FDA-regulated drug product

No

Studies a U.S. FDA-regulated device product

No

This information was retrieved directly from the website clinicaltrials.gov without any changes. If you have any requests to change, remove or update your study details, please contact register@clinicaltrials.gov. As soon as a change is implemented on clinicaltrials.gov, this will be updated automatically on our website as well.

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