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Adenosylmethionine Metabolism in Human Inflammation

torstai 6. elokuuta 2015 päivittänyt: En-Pei Isabel Chiang, National Chung Hsing University

Translational Study on the Regulation of Adenosylmethionine Synthesis During Chronic Inflammation

The investigators propose to conduct a translational study on the regulation of S-adenosylmethionine synthesis and cellular methylation reactions during chronic inflammation. Development of in vitro cell models may reveal the regulatory mechanisms by which specific inflammatory mediators cause metabolic changes and alter DNA methylation status. Metabolic and pharmacological studies in the in vivo models will enable us to better understand the regulation of inter-organ homeostasis of S-adenosyl methionine and help identify tissue specific biomarkers for methylation and epigenetic modifications in different stage of chronic inflammation. The clinical study in human subjects will help distinguish the impacts of autoimmune rheumatic disease, degenerated joint disease, or specific medication use on significant clinical and biochemical markers in folate and vitamin B6 metabolic pathways.The Investigators hope the present study can identify specific clinical markers for potential epigenetic changes in patients suffering from chronic inflammation, which will contribute to better clinical management of these diseases in humans.

Tutkimuksen yleiskatsaus

Tila

Tuntematon

Ehdot

Yksityiskohtainen kuvaus

The significance of epigenetic alterations in autoimmune rheumatic diseases and degenerated joint diseases has drawn great attention among clinicians and researchers. Aberrant methylation status has been demonstrated in human chronic inflammation yet more efforts have focused on global and sequence-specific hypomethylation and overexpression of specific genes. Few studies investigated the regulation of S-adenosylmethionine homeostasis and regulation during inflammation. At present the relevance and regulation of the complex epigenetic profiles and their modifications among different tissues and organs during inflammation remain largely unknown.

Opintotyyppi

Havainnollistava

Ilmoittautuminen (Odotettu)

250

Osallistumiskriteerit

Tutkijat etsivät ihmisiä, jotka sopivat tiettyyn kuvaukseen, jota kutsutaan kelpoisuuskriteereiksi. Joitakin esimerkkejä näistä kriteereistä ovat henkilön yleinen terveydentila tai aiemmat hoidot.

Kelpoisuusvaatimukset

Opintokelpoiset iät

18 vuotta - 80 vuotta (Aikuinen, Vanhempi Aikuinen)

Hyväksyy terveitä vapaaehtoisia

Sukupuolet, jotka voivat opiskella

Kaikki

Näytteenottomenetelmä

Todennäköisyysnäyte

Tutkimusväestö

patients with arthritis or healthy adults

Kuvaus

Inclusion Criteria:

> 18 years

Exclusion Criteria:

pregnancy,
anemia (hemoglobin 10 mg/dL or lower),
thrombocytopenia (platelet count below 50,000 cells/μL),
abnormal serum hepatic transaminase (aspartate aminotransferase or alanine aminotransferase above 50 IU/L),
diabetes or cancer

Opintosuunnitelma

Tässä osiossa on tietoja tutkimussuunnitelmasta, mukaan lukien kuinka tutkimus on suunniteltu ja mitä tutkimuksella mitataan.

Miten tutkimus on suunniteltu?

Ryhmien/kohorttien lukumäärä

Kohortit ja interventiot

Ryhmä/Kohortti
Arthritis subjects with arthritis
Health control subjects Health control

Mitä tutkimuksessa mitataan?

Ensisijaiset tulostoimenpiteet

Tulosmittaus	Toimenpiteen kuvaus	Aikaikkuna
s-adenosylmethionine Aikaikkuna: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
homocysteine Aikaikkuna: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
folate Aikaikkuna: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
vitamin B6 Aikaikkuna: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor

Toissijaiset tulostoimenpiteet

Tulosmittaus	Toimenpiteen kuvaus	Aikaikkuna
blood amino acid profile (serine, glycine, methionine,cysteine, cystathionine, dimethylglycine) Aikaikkuna: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
gene expression of target enzymes in PBMCs Aikaikkuna: Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses	Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor
enzyme activities of S-adenosylmethionine synthase in RBC Aikaikkuna: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
vitamin B6 metabolic enzyme in RBC Aikaikkuna: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses of above metabolites	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
polymorphisms in one carbon metabolism enzymes in PBMCs Aikaikkuna: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor	blood samples were collected and stored for later analyses	Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor

Yhteistyökumppanit ja tutkijat

Täältä löydät tähän tutkimukseen osallistuvat ihmiset ja organisaatiot.

Sponsori

National Chung Hsing University

Yhteistyökumppanit

National Science Council, Taiwan

Ministry of Science and Technology, Taiwan

Tutkijat

Päätutkija: En-Pei I Chiang, PhD, Department of Food Science and Biotechnology, National Chung Hsing University

Opintojen ennätyspäivät

Nämä päivämäärät seuraavat ClinicalTrials.gov-sivustolle lähetettyjen tutkimustietueiden ja yhteenvetojen edistymistä. National Library of Medicine (NLM) tarkistaa tutkimustiedot ja raportoidut tulokset varmistaakseen, että ne täyttävät tietyt laadunvalvontastandardit, ennen kuin ne julkaistaan julkisella verkkosivustolla.

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