Adenosylmethionine Metabolism in Human Inflammation
6 agosto 2015 aggiornato da: En-Pei Isabel Chiang, National Chung Hsing University
Translational Study on the Regulation of Adenosylmethionine Synthesis During Chronic Inflammation
The investigators propose to conduct a translational study on the regulation of S-adenosylmethionine synthesis and cellular methylation reactions during chronic inflammation.
Development of in vitro cell models may reveal the regulatory mechanisms by which specific inflammatory mediators cause metabolic changes and alter DNA methylation status.
Metabolic and pharmacological studies in the in vivo models will enable us to better understand the regulation of inter-organ homeostasis of S-adenosyl methionine and help identify tissue specific biomarkers for methylation and epigenetic modifications in different stage of chronic inflammation.
The clinical study in human subjects will help distinguish the impacts of autoimmune rheumatic disease, degenerated joint disease, or specific medication use on significant clinical and biochemical markers in folate and vitamin B6 metabolic pathways.The Investigators hope the present study can identify specific clinical markers for potential epigenetic changes in patients suffering from chronic inflammation, which will contribute to better clinical management of these diseases in humans.
Panoramica dello studio
Stato
Sconosciuto
Condizioni
Descrizione dettagliata
The significance of epigenetic alterations in autoimmune rheumatic diseases and degenerated joint diseases has drawn great attention among clinicians and researchers.
Aberrant methylation status has been demonstrated in human chronic inflammation yet more efforts have focused on global and sequence-specific hypomethylation and overexpression of specific genes.
Few studies investigated the regulation of S-adenosylmethionine homeostasis and regulation during inflammation.
At present the relevance and regulation of the complex epigenetic profiles and their modifications among different tissues and organs during inflammation remain largely unknown.
Tipo di studio
Osservativo
Iscrizione (Anticipato)
250
Criteri di partecipazione
I ricercatori cercano persone che corrispondano a una certa descrizione, chiamata criteri di ammissibilità. Alcuni esempi di questi criteri sono le condizioni generali di salute di una persona o trattamenti precedenti.
Criteri di ammissibilità
Età idonea allo studio
Da 18 anni a 80 anni (Adulto, Adulto più anziano)
Accetta volontari sani
No
Sessi ammissibili allo studio
Tutto
Metodo di campionamento
Campione di probabilità
Popolazione di studio
patients with arthritis or healthy adults
Descrizione
Inclusion Criteria:
- > 18 years
Exclusion Criteria:
- pregnancy,
- anemia (hemoglobin 10 mg/dL or lower),
- thrombocytopenia (platelet count below 50,000 cells/μL),
- abnormal serum hepatic transaminase (aspartate aminotransferase or alanine aminotransferase above 50 IU/L),
- diabetes or cancer
Piano di studio
Questa sezione fornisce i dettagli del piano di studio, compreso il modo in cui lo studio è progettato e ciò che lo studio sta misurando.
Come è strutturato lo studio?
Coorti e interventi
Gruppo / Coorte |
|---|
|
Arthritis
subjects with arthritis
|
|
Health control subjects
Health control
|
Cosa sta misurando lo studio?
Misure di risultato primarie
Misura del risultato |
Misura Descrizione |
Lasso di tempo |
|---|---|---|
|
s-adenosylmethionine
Lasso di tempo: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
|
homocysteine
Lasso di tempo: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
|
folate
Lasso di tempo: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
|
vitamin B6
Lasso di tempo: Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Some participants were followed for 4wks if medication was changed by the doctor
|
Misure di risultato secondarie
Misura del risultato |
Misura Descrizione |
Lasso di tempo |
|---|---|---|
|
blood amino acid profile (serine, glycine, methionine,cysteine, cystathionine, dimethylglycine)
Lasso di tempo: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
|
gene expression of target enzymes in PBMCs
Lasso di tempo: Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor
|
blood samples were collected and stored for later analyses
|
Blood were collected at admission.Blood were dawn again 1mo later if his medication was changed by the doctor
|
|
enzyme activities of S-adenosylmethionine synthase in RBC
Lasso di tempo: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
|
vitamin B6 metabolic enzyme in RBC
Lasso di tempo: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
blood samples were collected and stored for later analyses of above metabolites
|
Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
|
polymorphisms in one carbon metabolism enzymes in PBMCs
Lasso di tempo: Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
blood samples were collected and stored for later analyses
|
Blood were collected at admission. Blood were dawn again 1mo later if his medication was changed by the doctor
|
Collaboratori e investigatori
Qui è dove troverai le persone e le organizzazioni coinvolte in questo studio.
Sponsor
Investigatori
- Investigatore principale: En-Pei I Chiang, PhD, Department of Food Science and Biotechnology, National Chung Hsing University
Studiare le date dei record
Queste date tengono traccia dell'avanzamento della registrazione dello studio e dell'invio dei risultati di sintesi a ClinicalTrials.gov. I record degli studi e i risultati riportati vengono esaminati dalla National Library of Medicine (NLM) per assicurarsi che soddisfino specifici standard di controllo della qualità prima di essere pubblicati sul sito Web pubblico.
Studia le date principali
Inizio studio
1 gennaio 2011
Completamento primario (Effettivo)
1 luglio 2014
Completamento dello studio (Anticipato)
1 agosto 2015
Date di iscrizione allo studio
Primo inviato
28 luglio 2015
Primo inviato che soddisfa i criteri di controllo qualità
6 agosto 2015
Primo Inserito (Stima)
11 agosto 2015
Aggiornamenti dei record di studio
Ultimo aggiornamento pubblicato (Stima)
11 agosto 2015
Ultimo aggiornamento inviato che soddisfa i criteri QC
6 agosto 2015
Ultimo verificato
1 agosto 2015
Maggiori informazioni
Termini relativi a questo studio
Termini MeSH pertinenti aggiuntivi
Altri numeri di identificazione dello studio
- SF11093
Queste informazioni sono state recuperate direttamente dal sito web clinicaltrials.gov senza alcuna modifica. In caso di richieste di modifica, rimozione o aggiornamento dei dettagli dello studio, contattare register@clinicaltrials.gov. Non appena verrà implementata una modifica su clinicaltrials.gov, questa verrà aggiornata automaticamente anche sul nostro sito web .